PMID- 35281461
OWN - NLM
STAT- MEDLINE
DCOM- 20220324
LR  - 20220324
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2022
DP  - 2022
TI  - Tunneling Nanotube-Mediated Mitochondrial Transfer Rescues Nucleus Pulposus Cells 
      from Mitochondrial Dysfunction and Apoptosis.
PG  - 3613319
LID - 10.1155/2022/3613319 [doi]
LID - 3613319
AB  - Stem cell-based therapy has been indicated to be beneficial for intervertebral 
      disc regeneration. However, the underlying mechanisms have not been fully 
      identified. The present study showed that bone marrow mesenchymal stem cells 
      (BMSCs) donated mitochondria to adjacent nucleus pulposus cells (NPCs) in a 
      coculture system. The mode of mitochondrial transfer between these cells was 
      intercellular tunneling nanotube (TNT), which acted as a transportation 
      expressway for mitochondria. NPCs acquired additional mitochondria from BMSCs in 
      a concentration-dependent manner after rotenone-induced mitochondrial dysfunction 
      in NPCs. Further research demonstrated that TNT-mediated mitochondrial transfer 
      rescued NPCs from mitochondrial dysfunction and apoptosis, which was indicated by 
      the recovery of the mitochondrial respiratory chain, the increase in 
      mitochondrial membrane potential, and the decreases in reactive oxygen species 
      (ROS) levels and apoptosis rates. Furthermore, Miro1, a critical protein that 
      regulates mitochondrial movement, was knocked down in BMSCs and significantly 
      reduced mitochondrial transfer from BMSCs to NPCs. These results suggested that 
      Miro1 depletion inhibited the rescue of NPCs with mitochondrial dysfunction. 
      Taken together, our data shed light on a novel mechanism by which BMSCs rescue 
      impaired NPCs, providing a concrete foundation to study the critical role of 
      intercellular interactions in disc regeneration.
CI  - Copyright (c) 2022 Fan Yang et al.
FAU - Yang, Fan
AU  - Yang F
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Zhang, Yanbin
AU  - Zhang Y
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Liu, Sheng
AU  - Liu S
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Xiao, Jiheng
AU  - Xiao J
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - He, Yuxin
AU  - He Y
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Shao, Zengwu
AU  - Shao Z
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Zhang, Yuhui
AU  - Zhang Y
AD  - Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan 
      National Laboratory for Optoelectronics-Huazhong University of Science and 
      Technology, Wuhan 430074, China.
FAU - Cai, Xianyi
AU  - Cai X
AUID- ORCID: 0000-0002-8726-2067
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Xiong, Liming
AU  - Xiong L
AUID- ORCID: 0000-0002-9003-4671
AD  - Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
LA  - eng
PT  - Journal Article
DEP - 20220304
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Tunneling Nanotubes)
SB  - IM
MH  - Apoptosis
MH  - Cell Membrane Structures/*metabolism
MH  - Cells, Cultured
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mitochondria/*metabolism
MH  - Nanotubes
MH  - Nucleus Pulposus/*metabolism
PMC - PMC8916857
COIS- The authors declare no conflicts of interest.
EDAT- 2022/03/15 06:00
MHDA- 2022/03/25 06:00
PMCR- 2022/03/04
CRDT- 2022/03/14 05:22
PHST- 2021/12/08 00:00 [received]
PHST- 2022/01/28 00:00 [revised]
PHST- 2022/02/16 00:00 [accepted]
PHST- 2022/03/14 05:22 [entrez]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/03/25 06:00 [medline]
PHST- 2022/03/04 00:00 [pmc-release]
AID - 10.1155/2022/3613319 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2022 Mar 4;2022:3613319. doi: 10.1155/2022/3613319. 
      eCollection 2022.