PMID- 35282057
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220315
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 2
DP  - 2022 Jan
TI  - Influence of bFGF on in vitro expansion and chondrogenic construction of 
      articular cartilage-derived progenitor cells.
PG  - 36
LID - 10.21037/atm-21-5604 [doi]
LID - 36
AB  - BACKGROUND: Articular cartilage-derived progenitor cells (ACPCs) possess the 
      properties of both chondrocytes and bone marrow mesenchymal stem cells (BMSCs). 
      However, the number of ACPCs in articular cartilage is low, and an effective 
      culture system is needed for their expansion. Basic fibroblast growth factor 
      (bFGF) promotes the expansion of chondrocytes and BMSCs, as well as the 
      chondrogenic differentiation of BMSCs. Therefore, the aim of this study was to 
      clarify whether bFGF could be used for in vitro expansion of ACPCs and whether 
      bFGF promoted chondrogenic construction of ACPCs. METHODS: We applied the 
      fibronectin adhesion method to sort mice ACPCs and compared the proliferative, 
      osteogenic, and chondrogenic abilities of ACPCs by adding various concentration 
      of bFGF (0, 2, and 5 ng/mL) to the cell culture medium. Then used the best system 
      to construct cartilage with ACPCs in vitro and in vivo. RESULTS: The results 
      indicated that bFGF promoted ACPCs proliferation, inhibited osteogenesis, and 
      promoted chondrogenesis, and that a cell culture system containing 2 ng/mL bFGF 
      was optimal for these effects. ACPCs constructed cartilage using the filtered 
      culture system presented homogeneous cartilaginous histological structure in 
      vitro and in vivo. CONCLUSIONS: By applying this cell culture system, homogenous 
      cartilage tissue was constructed in vitro and in vivo by chondrogenic induction, 
      which provides a stable cell expansion culture method for the application of 
      ACPCs in cartilage repair.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Shen, Xu
AU  - Shen X
AD  - Department of Orthopedics, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
AD  - Department of Orthopedics, Suzhou Dushu Lake Hospital, Dushu Lake Hospital 
      Affiliated to Soochow University, Suzhou, China.
FAU - Zhu, Tianfeng
AU  - Zhu T
AD  - Department of Orthopedics, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
FAU - Xue, Jixin
AU  - Xue J
AD  - Department of Orthopedics, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, Wenzhou, China.
FAU - Zhang, Yijian
AU  - Zhang Y
AD  - Department of Orthopedics, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
FAU - Lu, Yingjie
AU  - Lu Y
AD  - Department of Orthopedics, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
FAU - Yang, Huilin
AU  - Yang H
AD  - Department of Orthopedics, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
FAU - Yu, Zhengyuan
AU  - Yu Z
AD  - Department of Oncology, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
FAU - Zhu, Yueqian
AU  - Zhu Y
AD  - Department of Dermatology, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
FAU - Zhu, Xuesong
AU  - Zhu X
AD  - Department of Orthopedics, the First Affiliated Hospital of Soochow University, 
      Suzhou, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8848382
OTO - NOTNLM
OT  - Articular cartilage-derived progenitor cells (ACPCs)
OT  - basic fibroblast growth factor (bFGF)
OT  - cartilage construction
OT  - cell expansion
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-21-5604/coif). The authors 
      have no conflicts of interest to declare.
EDAT- 2022/03/15 06:00
MHDA- 2022/03/15 06:01
PMCR- 2022/01/01
CRDT- 2022/03/14 05:30
PHST- 2021/04/06 00:00 [received]
PHST- 2022/01/05 00:00 [accepted]
PHST- 2022/03/14 05:30 [entrez]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/03/15 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - atm-10-02-36 [pii]
AID - 10.21037/atm-21-5604 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 Jan;10(2):36. doi: 10.21037/atm-21-5604.