PMID- 35282132
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220315
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 2
DP  - 2022 Jan
TI  - Effects of different concentrations of human umbilical cord mesenchymal stem 
      cells to ameliorate psoriasis-like skin lesions in BALB/c mice.
PG  - 86
LID - 10.21037/atm-22-4 [doi]
LID - 86
AB  - BACKGROUND: Psoriasis is an immune-mediated chronic, recurrent, inflammatory skin 
      disease. In view of the research on the relationship between stem cells and the 
      pathogenesis of psoriasis, stem cells may be a new breakthrough in the systemic 
      treatment of psoriasis. METHODS: The BALB/c mouse psoriasis-like model induced by 
      imiquimod was established and animals were randomly divided into a control group, 
      model group, human umbilical cord mesenchymal stem cells (hUC-MSCs) group with 
      different concentrations (injected separately with umbilical cord stem cells 
      1x10(7)/kg, 2x10(7)/kg, 4x10(7)/kg through the caudal vein) and fresh hUC-MSCs 
      group (injected with fresh umbilical cord stem cells 2x10(7)/kg through the tail 
      vein). The Psoriasis Area and Severity Index (PASI) score was used to observe the 
      changes in skin lesions. The epidermal thickness, degree of keratinization and 
      infiltration of inflammatory cells were observed by HE staining. The 
      concentrations of TNF-alpha, IFN-gamma, IL-17A, IL-23 and other cytokines in serum and 
      skin of mice were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: 
      Mice treated with hUC-MSCs showed a good dose-response dependence compared with 
      the control group. As the concentration of hUC-MSCs increased, so did the spleen 
      index. According to the PASI integral trend chart, hUC-MSCs can delay the 
      appearance of skin lesions and accelerate the recovery of skin lesions. HE 
      staining showed that the number of parakeratosis cells in the hUC-MSCs treatment 
      group was significantly decreased, and the degree of dermal hyperplasia and 
      inflammatory cell infiltration in erythrocyte extravasation was significantly 
      lower than in the model group. The higher the concentration of hUC-MSCs, the 
      lower the concentration of the four cytokines in serum and skin tissue. 
      CONCLUSIONS: hUC-MSCs had an obvious therapeutic effect on imiquimod-induced 
      psoriasis in mice, and a high concentration of hUC-MSCs had the best therapeutic 
      effect. This effect intensity is dose-dependent, and hUC-MSCs at high 
      concentrations have better therapeutic effect.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Lin, Youshu
AU  - Lin Y
AD  - Department of Cosmetic Dermatology, The Fifth People's Hospital of Hainan 
      Province, Haikou, China.
FAU - Wang, Hongwei
AU  - Wang H
AD  - Asia Stem Cell Regenerative Pharmaceutical Co., Ltd., Shanghai, China.
FAU - Jiang, Cuibao
AU  - Jiang C
AD  - Asia Stem Cell Regenerative Pharmaceutical Co., Ltd., Shanghai, China.
FAU - Chen, Chen
AU  - Chen C
AD  - Asia Stem Cell Regenerative Pharmaceutical Co., Ltd., Shanghai, China.
FAU - Shen, Danpeng
AU  - Shen D
AD  - Asia Stem Cell Regenerative Pharmaceutical Co., Ltd., Shanghai, China.
FAU - Xie, Fan
AU  - Xie F
AD  - Asia Stem Cell Regenerative Pharmaceutical Co., Ltd., Shanghai, China.
FAU - Zhang, Hongpeng
AU  - Zhang H
AD  - Asia Stem Cell Regenerative Pharmaceutical Co., Ltd., Shanghai, China.
FAU - Yang, Jie
AU  - Yang J
AD  - Department of Cosmetic Dermatology, The Fifth People's Hospital of Hainan 
      Province, Haikou, China.
FAU - Wang, Hao
AU  - Wang H
AD  - Asia Stem Cell Regenerative Pharmaceutical Co., Ltd., Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8848403
OTO - NOTNLM
OT  - Cytokines
OT  - human umbilical cord mesenchymal stem cells (hUC-MSCs)
OT  - indirubin
OT  - psoriasis
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-22-4/coif). The authors 
      report that research was supported by Hainan Province Clinical Medical Center and 
      the funding project "Effects of indigo naturalis and Arnebia on differentiation 
      and barrier function of epidermal stem cells in psoriasis response research 
      (Hainan Natural Science Foundation, 20158342)". HW, CJ, CC, DS, FX, HZ, HW are 
      from Asia Stem Cell Regenerative Pharmaceutical Co., Ltd. The authors have no 
      other conflicts of interest to declare.
EDAT- 2022/03/15 06:00
MHDA- 2022/03/15 06:01
PMCR- 2022/01/01
CRDT- 2022/03/14 05:30
PHST- 2021/11/12 00:00 [received]
PHST- 2022/01/19 00:00 [accepted]
PHST- 2022/03/14 05:30 [entrez]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/03/15 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - atm-10-02-86 [pii]
AID - 10.21037/atm-22-4 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 Jan;10(2):86. doi: 10.21037/atm-22-4.