PMID- 3528297
OWN - NLM
STAT- MEDLINE
DCOM- 19861001
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 92
IP  - 1
DP  - 1986 Aug 21
TI  - A sensitive and reproducible method for the determination of subnanogram 
      quantities of immunoglobulin E (IgE).
PG  - 131-6
AB  - An immunoradiometric assay for immunoglobulin E (IgE) using high adsorption 
      polystyrene test tubes as the solid phase (the 'Maxisorp' assay) is described. 
      The sensitivity of the method was found to be 50 pg IgE/ml and the within-assay 
      reproducibility was 3-7%. The accuracy was estimated by means of a comparison 
      between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a 
      correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude 
      that the Maxisorp assay is a fast and reliable assay for IgE determination in 
      cord blood, cell culture supernatants and other highly diluted IgE preparations.
FAU - Poulsen, L K
AU  - Poulsen LK
FAU - Malling, H J
AU  - Malling HJ
FAU - Sondergaard, I
AU  - Sondergaard I
FAU - Weeke, B
AU  - Weeke B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibody Specificity
MH  - Binding, Competitive
MH  - Cross Reactions
MH  - Dose-Response Relationship, Immunologic
MH  - Humans
MH  - Immunoglobulin A/analysis
MH  - Immunoglobulin E/*analysis
MH  - Immunoglobulin G/analysis
MH  - Immunoglobulin M/analysis
MH  - Immunosorbent Techniques
MH  - Radioimmunoassay/*methods
EDAT- 1986/08/21 00:00
MHDA- 1986/08/21 00:01
CRDT- 1986/08/21 00:00
PHST- 1986/08/21 00:00 [pubmed]
PHST- 1986/08/21 00:01 [medline]
PHST- 1986/08/21 00:00 [entrez]
AID - 0022-1759(86)90513-2 [pii]
AID - 10.1016/0022-1759(86)90513-2 [doi]
PST - ppublish
SO  - J Immunol Methods. 1986 Aug 21;92(1):131-6. doi: 10.1016/0022-1759(86)90513-2.