PMID- 35283260
OWN - NLM
STAT- MEDLINE
DCOM- 20220614
LR  - 20220627
IS  - 1096-7184 (Electronic)
IS  - 1096-7176 (Linking)
VI  - 72
DP  - 2022 Jul
TI  - Live isolation of naive ESCs via distinct glucose metabolism and stored glycogen.
PG  - 97-106
LID - S1096-7176(22)00039-8 [pii]
LID - 10.1016/j.ymben.2022.03.003 [doi]
AB  - Naive and primed pluripotent stem cells recapitulate the peri- and 
      post-implantation development, respectively. Thus, investigation of distinct 
      traits between each pluripotent stem cell type would shed light on early 
      embryonic processes. Herein, by screening a fluorescent probe library, we found 
      that intracellular glycogen led to specific reactivity to CDg4, a glycogen 
      fluorescence sensor, in both human and mouse naive embryonic stem cells (ESCs). 
      The requirement of constant inhibition of Gsk3beta as well as high oxidative 
      phosphorylation (OxPHOS) in naive compared to primed ESCs was closely associated 
      to high level of intracellular glycogen in naive ESCs. Both capacity of OxPHOS 
      and stored glycogen, rescued naive ESCs by transient inhibition of glycolysis, 
      which selectively eliminated primed ESCs. Additionally, naive ESCs with active 
      OxPHOS were enriched from a mixture with primed ESCs by high reactivity to 
      ATP-Red1, a mitochondrial ATP fluorescence probe. These results indicate the 
      active OxPHOS and high intracellular glycogen as a novel "biomarker" delineating 
      metabolic remodeling during the transition of naive pluripotency.
CI  - Copyright (c) 2022 International Metabolic Engineering Society. Published by 
      Elsevier Inc. All rights reserved.
FAU - Kim, Keun-Tae
AU  - Kim KT
AD  - Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 
      08826, Republic of Korea.
FAU - Oh, Ji-Young
AU  - Oh JY
AD  - College of Pharmacy, Seoul National University, Seoul, 08826, Republic of Korea.
FAU - Park, Seokwoo
AU  - Park S
AD  - Department of Biomedical Sciences, Seoul National University, College of 
      Medicine, Seoul, Republic of Korea.
FAU - Kim, Seong-Min
AU  - Kim SM
AD  - College of Pharmacy, Seoul National University, Seoul, 08826, Republic of Korea.
FAU - Benjamin, Patterson
AU  - Benjamin P
AD  - Department of Genetics, Yale Stem Cell Center, Child Study Center, 
      Interdepartmental Neuroscience Program, Yale School of Medicine, New Haven, CT, 
      06520, USA.
FAU - Park, In-Hyun
AU  - Park IH
AD  - Department of Genetics, Yale Stem Cell Center, Child Study Center, 
      Interdepartmental Neuroscience Program, Yale School of Medicine, New Haven, CT, 
      06520, USA.
FAU - Chun, Kwang-Hoon
AU  - Chun KH
AD  - Gachon Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon 
      University, Incheon, 21936, Republic of Korea.
FAU - Chang, Young-Tae
AU  - Chang YT
AD  - Department of Chemistry, Pohang University of Science and Technology (POSTECH), 
      Pohang, 37673, Republic of Korea; Center for Self-assembly and Complexity, 
      Institute for Basic Science (IBS), Pohang, 37673, Republic of Korea.
FAU - Cha, Hyuk-Jin
AU  - Cha HJ
AD  - Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, 
      08826, Republic of Korea; College of Pharmacy, Seoul National University, Seoul, 
      08826, Republic of Korea. Electronic address: hjcha93@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220310
PL  - Belgium
TA  - Metab Eng
JT  - Metabolic engineering
JID - 9815657
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9005-79-2 (Glycogen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - Embryonic Stem Cells/metabolism
MH  - Glucose/metabolism
MH  - *Glycogen/metabolism
MH  - Mice
MH  - *Pluripotent Stem Cells/metabolism
OTO - NOTNLM
OT  - Glucose metabolism
OT  - Glycogen
OT  - Mitochondrial ATP
OT  - Naive pluripotency
OT  - Primed pluripotency
EDAT- 2022/03/15 06:00
MHDA- 2022/06/15 06:00
CRDT- 2022/03/14 05:42
PHST- 2022/01/05 00:00 [received]
PHST- 2022/02/28 00:00 [revised]
PHST- 2022/03/07 00:00 [accepted]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/06/15 06:00 [medline]
PHST- 2022/03/14 05:42 [entrez]
AID - S1096-7176(22)00039-8 [pii]
AID - 10.1016/j.ymben.2022.03.003 [doi]
PST - ppublish
SO  - Metab Eng. 2022 Jul;72:97-106. doi: 10.1016/j.ymben.2022.03.003. Epub 2022 Mar 
      10.