PMID- 35284836
OWN - NLM
STAT- MEDLINE
DCOM- 20220422
LR  - 20220422
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 3
IP  - 1
DP  - 2022 Mar 18
TI  - In vivo optical recordings of ion dynamics in mouse corneal primary nociceptive 
      terminals.
PG  - 101224
LID - 10.1016/j.xpro.2022.101224 [doi]
LID - 101224
AB  - This protocol aims to measure ion dynamics in nociceptive terminal endings in 
      intact mice in vivo. We describe viral injection of GCaMP6s + RFP into trigeminal 
      ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive 
      terminals that express GCaMP6s and RFP. This fast and high-resolution optical 
      recording technique enables studying a nociceptive terminal's functional 
      molecular network in physiological and pathological conditions. This platform can 
      be applied to studying the physiology of terminals of other neurons. For complete 
      details on the use and execution of this protocol, please refer to Goldstein 
      et al. (2019).
CI  - (c) 2022 The Author(s).
FAU - Gershon, Devora
AU  - Gershon D
AD  - Department of Medical Neurobiology, Institute for Medical Research Israel Canada, 
      Faculty of Medicine, The Hebrew University, Jerusalem 9112001, Israel.
AD  - The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, 
      Jerusalem 9112001, Israel.
FAU - Negev-Goldstein, Robert H
AU  - Negev-Goldstein RH
AD  - Department of Medical Neurobiology, Institute for Medical Research Israel Canada, 
      Faculty of Medicine, The Hebrew University, Jerusalem 9112001, Israel.
AD  - The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, 
      Jerusalem 9112001, Israel.
FAU - Abd Al Razzaq, Lama
AU  - Abd Al Razzaq L
AD  - Department of Medical Neurobiology, Institute for Medical Research Israel Canada, 
      Faculty of Medicine, The Hebrew University, Jerusalem 9112001, Israel.
AD  - The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, 
      Jerusalem 9112001, Israel.
FAU - Lev, Shaya
AU  - Lev S
AD  - Department of Medical Neurobiology, Institute for Medical Research Israel Canada, 
      Faculty of Medicine, The Hebrew University, Jerusalem 9112001, Israel.
AD  - The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, 
      Jerusalem 9112001, Israel.
FAU - Binshtok, Alexander M
AU  - Binshtok AM
AD  - Department of Medical Neurobiology, Institute for Medical Research Israel Canada, 
      Faculty of Medicine, The Hebrew University, Jerusalem 9112001, Israel.
AD  - The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, 
      Jerusalem 9112001, Israel.
LA  - eng
GR  - CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220304
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Neurons
MH  - *Nociception
MH  - Trigeminal Ganglion/diagnostic imaging
PMC - PMC8904606
OTO - NOTNLM
OT  - Microscopy
OT  - Model Organisms
OT  - Neuroscience
COIS- The authors declare no competing interests.
EDAT- 2022/03/15 06:00
MHDA- 2022/04/23 06:00
PMCR- 2022/03/04
CRDT- 2022/03/14 06:01
PHST- 2022/03/14 06:01 [entrez]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/04/23 06:00 [medline]
PHST- 2022/03/04 00:00 [pmc-release]
AID - S2666-1667(22)00104-6 [pii]
AID - 101224 [pii]
AID - 10.1016/j.xpro.2022.101224 [doi]
PST - epublish
SO  - STAR Protoc. 2022 Mar 4;3(1):101224. doi: 10.1016/j.xpro.2022.101224. eCollection 
      2022 Mar 18.