PMID- 35285907
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220407
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Print)
IS  - 0168-6496 (Linking)
VI  - 98
IP  - 3
DP  - 2022 Mar 24
TI  - Succession of the wheat seed-associated microbiome as affected by soil fertility 
      level and introduction of Penicillium and Bacillus inoculants in the field.
LID - 10.1093/femsec/fiac028 [doi]
LID - fiac028
AB  - During germination, the seed releases nutrient-rich exudates into the 
      spermosphere, thereby fostering competition between resident microorganisms. 
      However, insight into the composition and temporal dynamics of seed-associated 
      bacterial communities under field conditions is currently lacking. This field 
      study determined the temporal changes from 11 to 31 days after sowing in the 
      composition of seed-associated bacterial communities of winter wheat as affected 
      by long-term soil fertilization history, and by introduction of the plant 
      growth-promoting microbial inoculants Penicillium bilaiae and Bacillus simplex. 
      The temporal dynamics were the most important factor affecting the composition of 
      the seed-associated communities. An increase in the relative abundance of genes 
      involved in organic nitrogen metabolism (ureC and gdhA), and in ammonium 
      oxidation (amoA), suggested increased mineralization of plant-derived nitrogen 
      compounds over time. Dynamics of the phosphorus cycling genes ppt, ppx and cphy 
      indicated inorganic phosphorus and polyphosphate cycling, as well as phytate 
      hydrolysis by the seed-associated bacteria early after germination. Later, an 
      increase in genes for utilization of organic phosphorus sources (phoD, phoX and 
      phnK) indicated phosphorus limitation. The results indicate that community 
      temporal dynamics are partly driven by changed availability of major nutrients, 
      and reveal no functional consequences of the added inoculants during seed 
      germination.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.
FAU - Nunes, Ines
AU  - Nunes I
AD  - Microbiomics and Microbe Discovery Denmark, Novozymes A/S, Biologiens Vej 2, 2880 
      Kgs Lyngby, Denmark.
FAU - Hansen, Veronika
AU  - Hansen V
AD  - Plant and Soil Section, Department of Plant and Environmental Sciences, Faculty 
      of Science, University of Copenhagen, Thorvaldsensevej 40, 1871 Frederiksberg C, 
      Denmark.
FAU - Bak, Frederik
AU  - Bak F
AUID- ORCID: 0000-0001-9580-8018
AD  - Section for Microbial Ecology and Biotechnology, Department of Plant and 
      Environmental Sciences, Faculty of Science, University of Copenhagen, 
      Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
FAU - Bonnichsen, Lise
AU  - Bonnichsen L
AD  - Section for Microbial Ecology and Biotechnology, Department of Plant and 
      Environmental Sciences, Faculty of Science, University of Copenhagen, 
      Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
FAU - Su, Jianqiang
AU  - Su J
AD  - Key Laboratory of Urban Environment and Health, Institute of Urban Environment, 
      Chinese Academy of Sciences, Xiamen 361021, China.
FAU - Hao, Xiuli
AU  - Hao X
AD  - Section for Microbial Ecology and Biotechnology, Department of Plant and 
      Environmental Sciences, Faculty of Science, University of Copenhagen, 
      Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University; Key Laboratory of Arable Land Conservation (Middle and Lower Reaches 
      of Yangtze River), Ministry of Agriculture, Huazhong Agricultural University, 
      Wuhan 430070, China.
FAU - Raymond, Nelly Sophie
AU  - Raymond NS
AD  - Plant and Soil Section, Department of Plant and Environmental Sciences, Faculty 
      of Science, University of Copenhagen, Thorvaldsensevej 40, 1871 Frederiksberg C, 
      Denmark.
AD  - School of Agriculture and Food Sciences, The University of Queensland, St Lucia, 
      Queensland 4072, Australia.
FAU - Nicolaisen, Mette Haubjerg
AU  - Nicolaisen MH
AUID- ORCID: 0000-0002-0983-2466
AD  - Section for Microbial Ecology and Biotechnology, Department of Plant and 
      Environmental Sciences, Faculty of Science, University of Copenhagen, 
      Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
FAU - Jensen, Lars Stoumann
AU  - Jensen LS
AD  - Plant and Soil Section, Department of Plant and Environmental Sciences, Faculty 
      of Science, University of Copenhagen, Thorvaldsensevej 40, 1871 Frederiksberg C, 
      Denmark.
FAU - Nybroe, Ole
AU  - Nybroe O
AD  - Section for Microbial Ecology and Biotechnology, Department of Plant and 
      Environmental Sciences, Faculty of Science, University of Copenhagen, 
      Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Fertilizers)
RN  - 0 (Soil)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - *Bacillus/genetics/metabolism
MH  - Fertilizers/analysis
MH  - *Microbiota
MH  - *Penicillium/metabolism
MH  - Phosphorus/metabolism
MH  - Seeds
MH  - Soil
MH  - Soil Microbiology
MH  - Triticum/microbiology
PMC - PMC8951222
OTO - NOTNLM
OT  - fertilizer amendment
OT  - long-term field trial
OT  - microbial inoculants
OT  - nitrogen and phosphorus cycling genes
OT  - seed microbiota
OT  - wheat seed microbiome
EDAT- 2022/03/15 06:00
MHDA- 2022/04/08 06:00
PMCR- 2022/03/14
CRDT- 2022/03/14 12:14
PHST- 2021/11/22 00:00 [received]
PHST- 2022/02/22 00:00 [revised]
PHST- 2022/03/10 00:00 [accepted]
PHST- 2022/03/15 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/03/14 12:14 [entrez]
PHST- 2022/03/14 00:00 [pmc-release]
AID - 6548193 [pii]
AID - fiac028 [pii]
AID - 10.1093/femsec/fiac028 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2022 Mar 24;98(3):fiac028. doi: 10.1093/femsec/fiac028.