PMID- 35287733
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220323
IS  - 1674-9782 (Print)
IS  - 2049-1891 (Electronic)
IS  - 1674-9782 (Linking)
VI  - 13
IP  - 1
DP  - 2022 Mar 15
TI  - Associations between testicular development and fetal size in the pig.
PG  - 24
LID - 10.1186/s40104-022-00678-3 [doi]
LID - 24
AB  - BACKGROUND: Impaired reproductive performance is the largest contributing factor 
      for the removal of boars from commercial systems. Intrauterine growth restricted 
      piglets represent 25% of the total number of piglets born and have impaired 
      reproductive performance. This study aimed to improve the understanding of 
      temporal changes in testicular gene expression during testes development in 
      fetuses of different size. The lightest and closest to mean litter weight (CTMLW) 
      male Large White x Landrace littermates were collected at gestational days (GD) 
      45, 60 and 90 (n = 5-6 litters/GD). RESULTS: Testes weight and testes weight as a 
      percentage of fetal weight were not associated with fetal size at GD60 or 90. 
      Fetal plasma testosterone was not associated with fetal size at GD90. There was 
      no association between fetal size and seminiferous tubule area and number, number 
      of germ or Sertoli cells per tubule. The lightest fetuses tended to have wider 
      seminiferous tubules compared to the CTMLW fetuses at GD90 (P = 0.077). The 
      testicular expression of KI67 (P </= 0.01) and BAX:BCL2 ratio (P = 0.058) mRNAs 
      decreased as gestation progressed. Greater SPP1 mRNA expression was observed at 
      GD60 when compared with GD45 and 90 (P </= 0.05). Lower expression of DMRT1 and 
      SPP1 (P < 0.01) mRNAs was observed in testes associated with the lightest fetuses 
      compared to the CTMLW fetuses at GD90. CONCLUSIONS: These findings provide novel 
      insights into the expression profiles of genes associated with testicular 
      development and function. Further, these data suggest that programming of 
      reproductive potential in IUGR boars occurs late in gestation, providing a 
      platform for further mechanistic investigation.
CI  - (c) 2022. The Author(s).
FAU - Stenhouse, Claire
AU  - Stenhouse C
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK. 
      claire.stenhouse@ag.tamu.edu.
AD  - Department of Animal Science, Texas A&M University, 440 Kleberg Center, College 
      Station, TX, 77843-2471, USA. claire.stenhouse@ag.tamu.edu.
FAU - Cortes-Araya, Yennifer
AU  - Cortes-Araya Y
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Donadeu, F Xavier
AU  - Donadeu FX
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Ashworth, Cheryl J
AU  - Ashworth CJ
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
LA  - eng
GR  - BB/J004316/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - Studentship/College of Medicine and Veterinary Medicine, University of Edinburgh/
GR  - 72170349/National Agency for Research and Development (ANID) / Scholarship 
      Program / DOCTORADO BECAS CHILE/2016/
PT  - Journal Article
DEP - 20220315
PL  - England
TA  - J Anim Sci Biotechnol
JT  - Journal of animal science and biotechnology
JID - 101581293
PMC - PMC8922848
OTO - NOTNLM
OT  - Fetal growth
OT  - Intrauterine growth restriction (IUGR)
OT  - Porcine
OT  - Pregnancy
OT  - Testes
COIS- The authors declare that there is no conflict of interest that could be perceived 
      as prejudicing the impartiality of the research reported.
EDAT- 2022/03/16 06:00
MHDA- 2022/03/16 06:01
PMCR- 2022/03/15
CRDT- 2022/03/15 05:36
PHST- 2021/10/14 00:00 [received]
PHST- 2022/01/09 00:00 [accepted]
PHST- 2022/03/15 05:36 [entrez]
PHST- 2022/03/16 06:00 [pubmed]
PHST- 2022/03/16 06:01 [medline]
PHST- 2022/03/15 00:00 [pmc-release]
AID - 10.1186/s40104-022-00678-3 [pii]
AID - 678 [pii]
AID - 10.1186/s40104-022-00678-3 [doi]
PST - epublish
SO  - J Anim Sci Biotechnol. 2022 Mar 15;13(1):24. doi: 10.1186/s40104-022-00678-3.