PMID- 35289351
OWN - NLM
STAT- MEDLINE
DCOM- 20220422
LR  - 20220716
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 42
IP  - 4
DP  - 2022 Apr 29
TI  - Anti-inflammatory activity of nicotine isolated from Brassica oleracea in 
      rheumatoid arthritis.
LID - 10.1042/BSR20211392 [doi]
LID - BSR20211392
AB  - OBJECTIVES: Rheumatoid arthritis (RA) is an autoimmune disease, associated with 
      chronic inflammation of synoviocytes. Tumor necrosis factor alpha (TNF-alpha) plays a 
      crucial role in the pathogenesis of RA through pro-inflammatory cytokines. 
      Nicotine, an alkaloid used as herbal medicine, often worked as an 
      anti-inflammatory agent. In the present study, we tried to uncover the 
      anti-inflammatory impact of nicotine against RA. MATERIALS AND METHODS: Nicotine 
      was isolated from Brassica oleracea, purified by high profile/phase liquid 
      chromatography (HPLC). In-silico docking was carried out using bioinformatics 
      tools SwissADME (absorption, distribution, metabolism and excretion), PASS, and 
      Drug-induced Gene Expression Profile (DIGEP)-Pred to determine drug likeliness of 
      nicotine. The in-vitro study was performed in TNFalpha-induced SW982 synoviocytes by 
      qPCR. mRNA expression of pro-inflammatory cytokines (TNF, IL6, IL1beta) and proteins 
      (TRAF2, P50, P65) were analyzed followed by validation of P65 (RELA), pP65, IkBalpha 
      by Western blot analysis. RESULTS: Nicotine compound was extracted from Brassica 
      oleracea and purified by HPLC method (Rt values at 2.67 min). The 
      physicochemical, pharmacokinetic properties and drug-likeliness of nicotine were 
      studied by in-silico analysis. In-vitro studies revealed that nicotine lowers the 
      expression of inflammatory cytokines (TNF, IL6, IL1beta) and proteins (TNF 
      receptor-associated factor 2 (TRAF2), P50, P65) at 1 microg/ml in TNFalpha-induced SW982 
      cells. CONCLUSION: Nicotine from natural sources (Brassica oleracea) has been 
      found to be an effective anti- inflammatory compound at a low dosage; thus, 
      identifying the role of nicotine present in the natural sources as a therapeutic 
      option for RA, may be recommended as remedial drug instead of synthetic drug.
CI  - (c) 2022 The Author(s).
FAU - Rakhecha, Bharti
AU  - Rakhecha B
AD  - Council of Industrial Research (CSIR)-Institute of Genomics and Integrative 
      Biology, Mall Road, Delhi University Campus, Delhi 110007, India.
FAU - Agnihotri, Prachi
AU  - Agnihotri P
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
FAU - Dakal, Tikam Chand
AU  - Dakal TC
AD  - Mohanlal Sukhadia University, Udaipur, Rajasthan 313001, India.
FAU - Saquib, Mohd
AU  - Saquib M
AD  - Council of Industrial Research (CSIR)-Institute of Genomics and Integrative 
      Biology, Mall Road, Delhi University Campus, Delhi 110007, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
FAU - Monu
AU  - Monu
AD  - Council of Industrial Research (CSIR)-Institute of Genomics and Integrative 
      Biology, Mall Road, Delhi University Campus, Delhi 110007, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
FAU - Biswas, Sagarika
AU  - Biswas S
AUID- ORCID: 0000-0002-2017-1670
AD  - Council of Industrial Research (CSIR)-Institute of Genomics and Integrative 
      Biology, Mall Road, Delhi University Campus, Delhi 110007, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 6M3C89ZY6R (Nicotine)
SB  - IM
MH  - Anti-Inflammatory Agents/pharmacology/therapeutic use
MH  - *Arthritis, Rheumatoid/pathology
MH  - *Brassica/metabolism
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Fibroblasts/metabolism
MH  - Interleukin-6/metabolism
MH  - Nicotine/pharmacology
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
PMC - PMC9069442
OTO - NOTNLM
OT  - HPLC
OT  - Nicotine
OT  - Rheumatoid arthritis
OT  - SW982 cells
OT  - TNFalpha
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2022/03/16 06:00
MHDA- 2022/04/23 06:00
PMCR- 2022/04/21
CRDT- 2022/03/15 08:43
PHST- 2021/06/11 00:00 [received]
PHST- 2022/02/24 00:00 [revised]
PHST- 2022/03/09 00:00 [accepted]
PHST- 2022/03/16 06:00 [pubmed]
PHST- 2022/04/23 06:00 [medline]
PHST- 2022/03/15 08:43 [entrez]
PHST- 2022/04/21 00:00 [pmc-release]
AID - 231028 [pii]
AID - BSR20211392 [pii]
AID - 10.1042/BSR20211392 [doi]
PST - ppublish
SO  - Biosci Rep. 2022 Apr 29;42(4):BSR20211392. doi: 10.1042/BSR20211392.