PMID- 35290310
OWN - NLM
STAT- MEDLINE
DCOM- 20220317
LR  - 20240214
IS  - 1539-4794 (Electronic)
IS  - 0146-9592 (Print)
IS  - 0146-9592 (Linking)
VI  - 47
IP  - 6
DP  - 2022 Mar 15
TI  - Multimodal imaging system combining optical coherence tomography and Brillouin 
      microscopy for neural tube imaging.
PG  - 1347-1350
LID - 10.1364/OL.453996 [doi]
AB  - To understand the dynamics of tissue stiffness during neural tube formation and 
      closure in a murine model, we have developed a multimodal, coaligned imaging 
      system combining optical coherence tomography (OCT) and Brillouin microscopy. 
      Brillouin microscopy can map the longitudinal modulus of tissue but cannot 
      provide structural images. Thus, it is limited for imaging dynamic processes such 
      as neural tube formation and closure. To overcome this limitation, we have 
      combined Brillouin microscopy and OCT in one coaligned instrument. OCT provided 
      depth-resolved structural imaging with a micrometer-scale spatial resolution to 
      guide stiffness mapping by Brillouin modality. 2D structural and Brillouin 
      frequency shift maps were acquired of mouse embryos at gestational day (GD) 8.5, 
      9.5, and 10.5 with the multimodal system. The results demonstrate the capability 
      of the system to obtain structural and stiffness information simultaneously.
FAU - Ambekar, Yogeshwari S
AU  - Ambekar YS
FAU - Singh, Manmohan
AU  - Singh M
FAU - Schill, Alexander W
AU  - Schill AW
FAU - Zhang, Jitao
AU  - Zhang J
FAU - Zevallos-Delgado, Christian
AU  - Zevallos-Delgado C
FAU - Khajavi, Behzad
AU  - Khajavi B
FAU - Aglyamov, Salavat R
AU  - Aglyamov SR
FAU - Finnell, Richard H
AU  - Finnell RH
FAU - Scarcelli, Giuliano
AU  - Scarcelli G
FAU - Larin, Kirill V
AU  - Larin KV
LA  - eng
GR  - K25 HD097288/HD/NICHD NIH HHS/United States
GR  - P30 EY007551/EY/NEI NIH HHS/United States
GR  - R01 EY030063/EY/NEI NIH HHS/United States
GR  - R01 HD095520/HD/NICHD NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Opt Lett
JT  - Optics letters
JID - 7708433
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Microscopy/methods
MH  - Multimodal Imaging
MH  - Neural Tube
MH  - *Tomography, Optical Coherence/methods
PMC - PMC9088521
MID - NIHMS1804149
EDAT- 2022/03/16 06:00
MHDA- 2022/03/18 06:00
PMCR- 2022/05/10
CRDT- 2022/03/15 17:18
PHST- 2022/03/15 17:18 [entrez]
PHST- 2022/03/16 06:00 [pubmed]
PHST- 2022/03/18 06:00 [medline]
PHST- 2022/05/10 00:00 [pmc-release]
AID - 470096 [pii]
AID - 10.1364/OL.453996 [doi]
PST - ppublish
SO  - Opt Lett. 2022 Mar 15;47(6):1347-1350. doi: 10.1364/OL.453996.