PMID- 35290477
OWN - NLM
STAT- MEDLINE
DCOM- 20220715
LR  - 20231031
IS  - 1436-6215 (Electronic)
IS  - 1436-6207 (Linking)
VI  - 61
IP  - 5
DP  - 2022 Aug
TI  - The daily caloric restriction and alternate-day fasting ameliorated lipid 
      dysregulation in type 2 diabetic mice by downregulating hepatic pescadillo 1.
PG  - 2775-2797
LID - 10.1007/s00394-022-02850-x [doi]
AB  - PURPOSE: A possible link between pescadillo 1 (PES1) and lipid metabolism has 
      been reported. However, whether PES1 is involved in the effects of daily caloric 
      restriction (CR) and alternate-day fasting (ADF) interventions on 
      diabetes-related lipid dysregulation is not elucidated. The current study aims 
      are to explore the role of PES1 in effects of CR and ADF on diabetic mice and 
      related mechanism. METHODS: Eight-week-old male db/db mice with type 2 diabetes 
      mellitus (T2DM) were randomly divided into untreated T2DM, CR and ADF groups. 
      McArdle hepatocytes were treated with 48 h high glucose (HG), 48 h normal glucose 
      (NG) and 24 h HG plus 24 h NG, respectively. Pes1 siRNA and overexpression 
      plasmid were, respectively, transfected into liver cells, and AAV(9)-Pes1-shRNA 
      was injected into db/db mice. RESULTS: After 12-week interventions, the 
      peroxisome proliferator-activated receptor alpha (PPAR-alpha) and carnitine 
      palmitoyltransferase 1A (CPT1A) levels in livers of T2DM mice were enhanced by CR 
      and ADF interventions with reductions of hepatic and plasma triglycerides. 
      Unexpectedly, hepatic PES1 levels were downregulated by two interventions, 
      consistent with the results of 48 h NG and 24 h HG plus 24 h NG-treated cells. 
      Moreover, CPT1A level was upregulated in Pes1-siRNA-treated cells and 
      AAV(9)-Pes1-shRNA injected murine livers, in contrast to Pes1 overexpression in 
      cultured cells. Mechanistically, 48 h NG or 24 h HG plus 24 h NG treatment 
      increased PPAR-alpha binding to Pes1 promoter, suppressing the PES1 expression, 
      thereby lowering the PES1-mediated ubiquitination of CPT1A. CONCLUSION: The 
      present study suggests that CR and ADF may improve lipid dysregulation in 
      diabetic mice by downregulating hepatic PES1.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.
FAU - Zhou, Jielin
AU  - Zhou J
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical 
      University, Hefei, 230032, Anhui, China.
FAU - Jiang, Zhengxuan
AU  - Jiang Z
AD  - Department of Ophthalmology, The Second Affiliated Hospital, Anhui Medical 
      University, Hefei, 230021, Anhui, China.
FAU - Lin, Yan
AU  - Lin Y
AD  - Department of Health Inspection and Quarantine, School of Public Health, Anhui 
      Medical University, Hefei, 230032, Anhui, China.
FAU - Li, Chengcheng
AU  - Li C
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical 
      University, Hefei, 230032, Anhui, China.
FAU - Liu, Juan
AU  - Liu J
AD  - Department of Health Inspection and Quarantine, School of Public Health, Anhui 
      Medical University, Hefei, 230032, Anhui, China.
FAU - Tian, Mengjun
AU  - Tian M
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical 
      University, Hefei, 230032, Anhui, China.
FAU - Liu, Yong
AU  - Liu Y
AD  - AIER Hefei Eye Hospital Affiliated To Anhui Medical University, Hefei, 230031, 
      Anhui, China. 18971021155@189.cn.
FAU - Chen, Keyang
AU  - Chen K
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical 
      University, Hefei, 230032, Anhui, China. chenkeyang@ahmu.edu.cn.
AD  - Department of Health Inspection and Quarantine, School of Public Health, Anhui 
      Medical University, Hefei, 230032, Anhui, China. chenkeyang@ahmu.edu.cn.
LA  - eng
GR  - 81570786/National Natural Science Foundation of China/
GR  - 82070986/National Natural Science Foundation of China/
GR  - 9101041203/Wanjiang scholar grants from Anhui Province of China/
PT  - Journal Article
DEP - 20220315
PL  - Germany
TA  - Eur J Nutr
JT  - European journal of nutrition
JID - 100888704
RN  - 0 (Peroxisome Proliferator-Activated Receptors)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Triglycerides)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Caloric Restriction
MH  - *Diabetes Mellitus, Experimental/metabolism
MH  - *Diabetes Mellitus, Type 2/metabolism/therapy
MH  - Fasting/physiology
MH  - Glucose/metabolism
MH  - Lipid Metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Peroxisome Proliferator-Activated Receptors/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Triglycerides/metabolism
OTO - NOTNLM
OT  - Alternate-day fasting (ADF)
OT  - Daily caloric restriction (CR)
OT  - Glucose metabolism
OT  - Lipid metabolism
OT  - Pescadillo 1 (PES1)
OT  - Type 2 diabetes (T2DM)
EDAT- 2022/03/16 06:00
MHDA- 2022/07/16 06:00
CRDT- 2022/03/15 17:19
PHST- 2021/09/24 00:00 [received]
PHST- 2022/02/22 00:00 [accepted]
PHST- 2022/03/16 06:00 [pubmed]
PHST- 2022/07/16 06:00 [medline]
PHST- 2022/03/15 17:19 [entrez]
AID - 10.1007/s00394-022-02850-x [pii]
AID - 10.1007/s00394-022-02850-x [doi]
PST - ppublish
SO  - Eur J Nutr. 2022 Aug;61(5):2775-2797. doi: 10.1007/s00394-022-02850-x. Epub 2022 
      Mar 15.