PMID- 35291619
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220318
IS  - 2322-3480 (Print)
IS  - 2322-3480 (Electronic)
IS  - 2322-3480 (Linking)
VI  - 10
IP  - 4
DP  - 2022 Jan
TI  - Potential Role of circRNA-HIPK3/microRNA-124a Crosstalk in the Pathogenesis of 
      Rheumatoid Arthritis.
PG  - 527-536
LID - 10.52547/rbmb.10.4.527 [doi]
AB  - BACKGROUND: Circular RNA-HIPK3 (CircHIPK3) has been shown to be aberrantly 
      expressed in a variety of diseases, contributing to disease initiation and 
      progression. The aim of the present study is to investigate the role of the 
      circHIPK3 RNA/microRNA-124a interaction in the pathogenesis of rheumatoid 
      arthritis (RA). METHODS: This study included 79 RA patients and 30 control 
      individuals. The patients involved were classified according to the disease 
      activity score (DAS28) into mild (24 patients), moderate (24 patients), and 
      severe (31 patients). Serum samples were collected to estimate the relative gene 
      expression of circHIPK3 RNA and its target gene microRNA-124a by quantitative 
      real time-PCR. Moreover, ELISA was used to detect the serum levels of monocyte 
      chemoattractant protein-1 (MCP-1). Routine laboratory estimation of erythrocyte 
      sedimentation rate (ESR), C-reactive protein (CRP), and rheumatoid factor (RF) 
      was also done. RESULTS: In all grades of RA groups, there was a significantly 
      substantial elevation of circHIPK3 RNA gene expression, with subsequent 
      downregulation of miRNA-124a when compared to the control group. CircHIPK3 and 
      microRNA-124a expression have been established to be inversely linked. Also, 
      estimation of serum levels of MCP-1, ESR, CRP, and RF exhibited a significant 
      increase in all grades of RA as compared to the control group. CONCLUSION: 
      CircHIPK3 and microRNA-124a might be regarded as key players in the pathogenesis 
      of RA. The cross-talk between them appears to be responsible for inducing joint 
      inflammation by increasing MCP-1 production. Targeting circHIPK3 and 
      microRNA-124a, and their downstream adaptor molecules, poses a new challenge for 
      RA therapy.
FAU - Saad El-Din, Shimaa
AU  - Saad El-Din S
AD  - The Department of Medical Biochemistry and Molecular Biology, Faculty of 
      Medicine, Cairo University, Cairo, Egypt.
FAU - Ahmed Rashed, Laila
AU  - Ahmed Rashed L
AD  - The Department of Medical Biochemistry and Molecular Biology, Faculty of 
      Medicine, Cairo University, Cairo, Egypt.
FAU - Eissa, Mervat
AU  - Eissa M
AD  - The Department of Rheumatology and Rehabilitation, Faculty of Medicine, Cairo 
      University, Cairo, Egypt.
FAU - Eldemery, Ahmed Bahgat
AU  - Eldemery AB
AD  - The Department of Medical Biochemistry and Molecular Biology, Faculty of 
      Medicine, October 6: University, Cairo, Egypt.
FAU - Abdelkareem Mohammed, Omnia
AU  - Abdelkareem Mohammed O
AD  - The Department of Medical Biochemistry and Molecular Biology, Faculty of 
      Medicine, October 6: University, Cairo, Egypt.
FAU - Abdelgwad, Marwa
AU  - Abdelgwad M
AD  - The Department of Medical Biochemistry and Molecular Biology, Faculty of 
      Medicine, Cairo University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Rep Biochem Mol Biol
JT  - Reports of biochemistry & molecular biology
JID - 101637937
PMC - PMC8903361
OTO - NOTNLM
OT  - Circhipk3
OT  - Circular RNA
OT  - Microrna-124a
OT  - Rheumatoid arthritis
EDAT- 2022/03/17 06:00
MHDA- 2022/03/17 06:01
PMCR- 2022/01/01
CRDT- 2022/03/16 05:12
PHST- 2021/10/05 00:00 [received]
PHST- 2021/10/19 00:00 [accepted]
PHST- 2022/03/16 05:12 [entrez]
PHST- 2022/03/17 06:00 [pubmed]
PHST- 2022/03/17 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - rbmb-10-527 [pii]
AID - 10.52547/rbmb.10.4.527 [doi]
PST - ppublish
SO  - Rep Biochem Mol Biol. 2022 Jan;10(4):527-536. doi: 10.52547/rbmb.10.4.527.