PMID- 35292250
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220407
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 921
DP  - 2022 Apr 15
TI  - Peptides derived from sea cucumber accelerate cells proliferation and migration 
      for wound healing by promoting energy metabolism and upregulating the ERK/AKT 
      pathway.
PG  - 174885
LID - S0014-2999(22)00146-7 [pii]
LID - 10.1016/j.ejphar.2022.174885 [doi]
AB  - Numerous bioactive peptides have high potential in accelerating skin repair. This 
      study aimed to explore wound healing capacity of sea cucumber-derived 
      tetrapeptides with amino acid sequence of Val-Thr-Pro-Tyr (VTPY) and 
      Val-Leu-Leu-Tyr (VLLY) by human skin fibroblast (HSF) and human umbilical vein 
      endothelial cells (HUVEC) in vitro. The results showed that VTPY and VLLY 
      possessed excellent capacity to induce the proliferation and migration of HSF 
      cells and HUVEC cells. Among them, VTPY possessed a higher cell 
      migration-promotion capacity than VLLY. Meanwhile, VTPY and VLLY increased 
      mitochondrial respiratory capacity of cells, leading to the significant increase 
      in adenosine triphosphate (ATP) production. Additionally, VTPY and VLLY 
      significantly increased the percentage of S phase in cell cycle of HUVEC via 
      upregulation of extracellular regulated protein kinases (ERK) and protein kinase 
      B (AKT) signaling pathway. Molecular docking further indicated the interaction of 
      peptides with mitogen-activated protein kinase phosphatase (MKP) and PH domain 
      leucine-rich repeat protein phosphatase (PHLPP) binding site in the ERK2 and AKT, 
      respectively, leading to subsequent increased phosphorylation of ERK1/2 and AKT. 
      Overall, peptides of VTPY and VLLY possessed outstanding capacity to induce the 
      proliferation and migration of HSF cells and HUVEC cells in vitro and the 
      mechanism was mainly related to improving mitochondrial respiratory capacity to 
      produce more ATP for biological energy, blocking the binding of MKP to ERK2 and 
      PHLPP to AKT and thus upregulating the ERK/AKT pathway.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Zheng, Zhihong
AU  - Zheng Z
AD  - National Engineering Research Center of Seafood, School of Food Science and 
      Technology, Dalian Polytechnic University, Dalian, 116034, PR China.
FAU - Li, Mengqi
AU  - Li M
AD  - National Engineering Research Center of Seafood, School of Food Science and 
      Technology, Dalian Polytechnic University, Dalian, 116034, PR China.
FAU - Jiang, Pengfei
AU  - Jiang P
AD  - National Engineering Research Center of Seafood, School of Food Science and 
      Technology, Dalian Polytechnic University, Dalian, 116034, PR China; 
      Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic 
      University, Dalian, 116034, PR China.
FAU - Sun, Na
AU  - Sun N
AD  - National Engineering Research Center of Seafood, School of Food Science and 
      Technology, Dalian Polytechnic University, Dalian, 116034, PR China; 
      Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic 
      University, Dalian, 116034, PR China. Electronic address: sunna1215@126.com.
FAU - Lin, Songyi
AU  - Lin S
AD  - National Engineering Research Center of Seafood, School of Food Science and 
      Technology, Dalian Polytechnic University, Dalian, 116034, PR China; 
      Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic 
      University, Dalian, 116034, PR China. Electronic address: linsongyi730@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220312
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Peptides)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Energy Metabolism
MH  - Human Umbilical Vein Endothelial Cells/metabolism
MH  - Humans
MH  - Molecular Docking Simulation
MH  - Peptides/metabolism/pharmacology
MH  - *Proto-Oncogene Proteins c-akt/metabolism
MH  - *Sea Cucumbers/metabolism
MH  - Wound Healing
OTO - NOTNLM
OT  - Energy metabolism
OT  - HSF cells
OT  - HUVEC cells
OT  - Molecular docking
OT  - Sea cucumber derived peptide
OT  - Wound healing
EDAT- 2022/03/17 06:00
MHDA- 2022/04/08 06:00
CRDT- 2022/03/16 05:42
PHST- 2021/10/08 00:00 [received]
PHST- 2022/03/03 00:00 [revised]
PHST- 2022/03/09 00:00 [accepted]
PHST- 2022/03/17 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/03/16 05:42 [entrez]
AID - S0014-2999(22)00146-7 [pii]
AID - 10.1016/j.ejphar.2022.174885 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2022 Apr 15;921:174885. doi: 10.1016/j.ejphar.2022.174885. Epub 
      2022 Mar 12.