PMID- 35294793
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20230124
IS  - 1600-6143 (Electronic)
IS  - 1600-6135 (Print)
IS  - 1600-6135 (Linking)
VI  - 22
IP  - 7
DP  - 2022 Jul
TI  - Macrophage-inducible C-type lectin activates B cells to promote T cell 
      reconstitution in heart allograft recipients.
PG  - 1779-1790
LID - 10.1111/ajt.17033 [doi]
AB  - Diminishing homeostatic proliferation of memory T cells is essential for 
      improving the efficacy of lymphoablation in transplant recipients. Our previous 
      studies in a mouse heart transplantation model established that B lymphocytes 
      secreting proinflammatory cytokines are critical for T cell recovery after 
      lymphoablation. The goal of the current study was to identify mediators of B cell 
      activation following lymphoablation in allograft recipients. Transcriptome 
      analysis revealed that macrophage-inducible C-type lectin (Mincle, Clec4e) 
      expression is up-regulated in B cells from heart allograft recipients treated 
      with murine anti-thymocyte globulin (mATG). Recipient Mincle deficiency 
      diminishes B cell production of pro-inflammatory cytokines and impairs T 
      lymphocyte reconstitution. Mixed bone marrow chimeras lacking Mincle only in B 
      lymphocytes have similar defects in T cell recovery. Conversely, treatment with a 
      synthetic Mincle ligand enhances T cell reconstitution after lymphoablation in 
      non-transplanted mice. Treatment with agonistic CD40 mAb facilitates T cell 
      reconstitution in CD4 T cell-depleted, but not in Mincle-deficient, recipients 
      indicating that CD40 signaling induces T cell proliferation via a 
      Mincle-dependent pathway. These findings are the first to identify an important 
      function of B cell Mincle as a sensor of damage-associated molecular patterns 
      released by the graft and demonstrate its role in clinically relevant settings of 
      organ transplantation.
CI  - (c) 2022 The Authors. American Journal of Transplantation published by Wiley 
      Periodicals LLC on behalf of The American Society of Transplantation and the 
      American Society of Transplant Surgeons.
FAU - Hasgur, Suheyla
AU  - Hasgur S
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
FAU - Yamamoto, Yosuke
AU  - Yamamoto Y
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
FAU - Fan, Ran
AU  - Fan R
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
FAU - Nicosia, Michael
AU  - Nicosia M
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
FAU - Gorbacheva, Victoria
AU  - Gorbacheva V
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
FAU - Zwick, Daniel
AU  - Zwick D
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
FAU - Araki, Motoo
AU  - Araki M
AD  - Department of Urology, Okayama University Graduate School of Medicine, Dentistry 
      and Pharmaceutical Sciences, Okayama, Japan.
FAU - Fairchild, Robert L
AU  - Fairchild RL
AUID- ORCID: 0000-0002-7107-5259
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
FAU - Valujskikh, Anna
AU  - Valujskikh A
AUID- ORCID: 0000-0003-3252-0636
AD  - Department of Inflammation and Immunity, Lerner Research Institute, Cleveland 
      Clinic, Cleveland, Ohio, USA.
LA  - eng
GR  - R01 AI113142/AI/NIAID NIH HHS/United States
GR  - U01 AI063594/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220401
PL  - United States
TA  - Am J Transplant
JT  - American journal of transplantation : official journal of the American Society of 
      Transplantation and the American Society of Transplant Surgeons
JID - 100968638
RN  - 0 (Cytokines)
RN  - 0 (Lectins, C-Type)
SB  - IM
MH  - Allografts
MH  - Animals
MH  - *B-Lymphocytes/metabolism
MH  - Cytokines/metabolism
MH  - *Heart Transplantation
MH  - Lectins, C-Type/metabolism
MH  - Macrophages
MH  - Mice
MH  - Mice, Inbred C57BL
PMC - PMC9296143
MID - NIHMS1820892
OTO - NOTNLM
OT  - B cell biology
OT  - T cell biology
OT  - basic (laboratory) research/science
OT  - immunobiology
OT  - immunosuppressive regimens - induction
OT  - lymphocyte biology
OT  - solid organ transplantation
EDAT- 2022/03/17 06:00
MHDA- 2022/07/07 06:00
PMCR- 2022/10/07
CRDT- 2022/03/16 17:18
PHST- 2022/02/21 00:00 [revised]
PHST- 2021/11/11 00:00 [received]
PHST- 2022/03/12 00:00 [accepted]
PHST- 2022/03/17 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
PHST- 2022/03/16 17:18 [entrez]
PHST- 2022/10/07 00:00 [pmc-release]
AID - S1600-6135(22)08267-3 [pii]
AID - AJT17033 [pii]
AID - 10.1111/ajt.17033 [doi]
PST - ppublish
SO  - Am J Transplant. 2022 Jul;22(7):1779-1790. doi: 10.1111/ajt.17033. Epub 2022 Apr 
      1.