PMID- 35296723
OWN - NLM
STAT- MEDLINE
DCOM- 20220504
LR  - 20221022
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Mar 16
TI  - Phenotypic, molecular and biochemical evaluation of somatic hybrids between 
      Solanum tuberosum and S. bulbocastanum.
PG  - 4484
LID - 10.1038/s41598-022-08424-5 [doi]
LID - 4484
AB  - Somatic hybridization has been frequently used to overcome sexual incompatibility 
      between potato and its secondary germplasm. The primary objective of this study 
      was to produce and evaluate somatic hybrids of Solanum tuberosum (Stub) and S. 
      bulbocastanum (Sblb) for breeding purposes. In 2007, 23 somatic hybrids were 
      produced using an electrofusion of mesophyll protoplasts of diploid 
      (2n = 2x = 24) potato line StubDH165 and S. bulbocastanum PI24351 (Sblb66). 
      Phenotype of somatic hybrids in field conditions were evaluated, together with 
      constitution and stability of 30 nuclear (ncSSR) and 27 cytoplasmic (cpSSR) 
      microsatellite markers and content of main glycoalkaloids. All somatic hybrids 
      had very high field resistance against late blight, but the plants were 
      infertile: the viability of pollen grains insignificantly varied between 0.58 and 
      8.97%. A significant somaclonal variation was observed in terms of the morphology 
      of plants, the date of emergence, the quantity of harvested tubers, the content 
      of glycoalkaloids in foliage, and nuclear microsatellite markers (ncSSR). The 
      analysis of ncSSR identified five distinct genotypes of hybrids partly associated 
      with phenotype variations. The process of somatic hybridization with regeneration 
      of shoots was identified as the most likely source of somaclonal variation 
      because the ncSSR genotypes of hybrids, which were maintained in vitro, remained 
      stable for more than 10 years. The infertile somatic hybrids have no practical 
      breeding potential, but they are considered very suitable for advanced studies of 
      the differential expression of genes in the pathways linked to dormancy of tubers 
      and synthesis of glycoalkaloids.
CI  - (c) 2022. The Author(s).
FAU - Sedlak, Petr
AU  - Sedlak P
AUID- ORCID: 0000-0001-8016-8900
AD  - Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural 
      Resources, Czech University of Life Sciences Prague, Kamycka 129, 16500, Prague 6 
      Suchdol, Czech Republic. sedlak@af.czu.cz.
FAU - Sedlakova, Vladimira
AU  - Sedlakova V
AUID- ORCID: 0000-0003-4553-5108
AD  - Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural 
      Resources, Czech University of Life Sciences Prague, Kamycka 129, 16500, Prague 6 
      Suchdol, Czech Republic.
FAU - Vasek, Jakub
AU  - Vasek J
AUID- ORCID: 0000-0002-9781-7708
AD  - Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural 
      Resources, Czech University of Life Sciences Prague, Kamycka 129, 16500, Prague 6 
      Suchdol, Czech Republic.
FAU - Zeka, Dukagjin
AU  - Zeka D
AUID- ORCID: 0000-0002-1757-2350
AD  - Department of Crops, University of Prishtina, Str. "George Bush" 31, 10 000, 
      Pristina, Republic of Kosovo.
FAU - Cilova, Daniela
AU  - Cilova D
AD  - Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural 
      Resources, Czech University of Life Sciences Prague, Kamycka 129, 16500, Prague 6 
      Suchdol, Czech Republic.
FAU - Melounova, Martina
AU  - Melounova M
AUID- ORCID: 0000-0001-9383-962X
AD  - Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural 
      Resources, Czech University of Life Sciences Prague, Kamycka 129, 16500, Prague 6 
      Suchdol, Czech Republic.
FAU - Orsak, Matyas
AU  - Orsak M
AUID- ORCID: 0000-0001-9706-2846
AD  - Department of Chemistry, Faculty of Agrobiology, Food and Natural Resources, 
      Czech University of Life Sciences Prague, Kamycka 129, 16500, Prague 6 Suchdol, 
      Czech Republic.
FAU - Domkarova, Jaroslava
AU  - Domkarova J
AD  - Department of Genetic Resources, Potato Research Institute Havlickuv Brod, Ltd., 
      Dobrovskeho 2366, 58001, Havlickuv Brod, Czech Republic.
FAU - Dolezal, Petr
AU  - Dolezal P
AUID- ORCID: 0000-0001-5966-3149
AD  - Department of Potato Protection, Potato Research Institute Havlickuv Brod, Ltd., 
      Dobrovskeho 2366, 58001, Havlickuv Brod, Czech Republic.
FAU - Vejl, Pavel
AU  - Vejl P
AUID- ORCID: 0000-0001-8199-8308
AD  - Department of Genetics and Breeding, Faculty of Agrobiology, Food and Natural 
      Resources, Czech University of Life Sciences Prague, Kamycka 129, 16500, Prague 6 
      Suchdol, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220316
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Diploidy
MH  - Phenotype
MH  - Plant Breeding
MH  - Plant Tubers/genetics
MH  - *Solanum/genetics
MH  - *Solanum tuberosum/chemistry/genetics
PMC - PMC8927101
COIS- The authors declare no competing interests.
EDAT- 2022/03/18 06:00
MHDA- 2022/05/06 06:00
PMCR- 2022/03/16
CRDT- 2022/03/17 05:32
PHST- 2021/10/01 00:00 [received]
PHST- 2022/02/28 00:00 [accepted]
PHST- 2022/03/17 05:32 [entrez]
PHST- 2022/03/18 06:00 [pubmed]
PHST- 2022/05/06 06:00 [medline]
PHST- 2022/03/16 00:00 [pmc-release]
AID - 10.1038/s41598-022-08424-5 [pii]
AID - 8424 [pii]
AID - 10.1038/s41598-022-08424-5 [doi]
PST - epublish
SO  - Sci Rep. 2022 Mar 16;12(1):4484. doi: 10.1038/s41598-022-08424-5.