PMID- 35298165
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220401
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 70
IP  - 12
DP  - 2022 Mar 30
TI  - Reconstruction of a Cofactor Self-Sufficient Whole-Cell Biocatalyst System for 
      Efficient Biosynthesis of Allitol from d-Glucose.
PG  - 3775-3784
LID - 10.1021/acs.jafc.2c00440 [doi]
AB  - The combined catalysis of glucose isomerase (GI), d-psicose 3-epimerase (DPEase), 
      ribitol dehydrogenase (RDH), and formate dehydrogenase (FDH) provides a 
      convenient route for the biosynthesis of allitol from d-glucose; however, the low 
      catalytic efficiency restricts its industrial applications. Here, the 
      supplementation of 0.32 g/L NAD(+) significantly promoted the cell catalytic 
      activity by 1.18-fold, suggesting that the insufficient intracellular NAD(H) 
      content was a limiting factor in allitol production. Glucose dehydrogenase (GDH) 
      with 18.13-fold higher activity than FDH was used for reconstructing a cofactor 
      self-sufficient system, which was combined with the overexpression of the 
      rate-limiting genes involved in NAD(+) salvage metabolic flow to expand the 
      available intracellular NAD(H) pool. Then, the multienzyme self-assembly system 
      with SpyTag and SpyCatcher effectively channeled intermediates, leading to an 
      81.1% increase in allitol titer to 15.03 g/L from 25 g/L d-glucose. This study 
      provided a facilitated strategy for large-scale and efficient biosynthesis of 
      allitol from a low-cost substrate.
FAU - Zhao, Jingyi
AU  - Zhao J
AD  - College of Light Industry and Food Engineering, Guangxi University, 100 Daxue 
      Road, Nanning, Guangxi 530004, China.
FAU - Guo, Yan
AU  - Guo Y
AD  - College of Light Industry and Food Engineering, Guangxi University, 100 Daxue 
      Road, Nanning, Guangxi 530004, China.
FAU - Li, Qiufeng
AU  - Li Q
AD  - College of Light Industry and Food Engineering, Guangxi University, 100 Daxue 
      Road, Nanning, Guangxi 530004, China.
FAU - Chen, Jing
AU  - Chen J
AD  - South Subtropical Agricultural Scientific Research Institute of Guangxi, 
      Longzhou, Guangxi 532415, China.
FAU - Niu, Debao
AU  - Niu D
AD  - College of Light Industry and Food Engineering, Guangxi University, 100 Daxue 
      Road, Nanning, Guangxi 530004, China.
FAU - Liu, Jidong
AU  - Liu J
AUID- ORCID: 0000-0003-3269-1240
AD  - College of Light Industry and Food Engineering, Guangxi University, 100 Daxue 
      Road, Nanning, Guangxi 530004, China.
LA  - eng
PT  - Journal Article
DEP - 20220317
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Sugar Alcohols)
RN  - B3F996O6JU (allitol)
RN  - EC 1.17.1.9 (Formate Dehydrogenases)
RN  - EC 5.1.- (Racemases and Epimerases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Formate Dehydrogenases/genetics
MH  - *Glucose
MH  - Racemases and Epimerases
MH  - *Sugar Alcohols/metabolism
OTO - NOTNLM
OT  - cofactor regeneration
OT  - multienzyme cascade catalysis
OT  - self-assembly
OT  - whole-cell biotransformation
EDAT- 2022/03/18 06:00
MHDA- 2022/04/01 06:00
CRDT- 2022/03/17 17:16
PHST- 2022/03/18 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2022/03/17 17:16 [entrez]
AID - 10.1021/acs.jafc.2c00440 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2022 Mar 30;70(12):3775-3784. doi: 10.1021/acs.jafc.2c00440. 
      Epub 2022 Mar 17.