PMID- 35298928
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220407
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 243
DP  - 2022 Jun 1
TI  - Portable fully automated oral fluid extraction device for illegal drugs.
PG  - 123389
LID - S0039-9140(22)00185-0 [pii]
LID - 10.1016/j.talanta.2022.123389 [doi]
AB  - Currently used methods for in-field determination of illegal drugs involve 
      various test kits based mainly on the immunoassay technique, where the presence 
      of a compound of interest is assessed by antibody-antigen reaction and manifested 
      by observable color change. Despite being accepted and widely used by police 
      forces to test the presence of illegal drugs in a suspect person, these tests 
      often suffer from unreliable results (high level of false-positive and/or 
      false-negative) due to the cross-reactivity and difficulties with quantification. 
      Therefore, we have developed a portable capillary electrophoresis instrument to 
      determine illegal drugs in oral fluid collected from a suspected person. However, 
      this drug analyzer has still required manual sample preparation. Therefore, this 
      research aimed to develop, test, and validate a fully automated sample 
      pretreatment (purification, extraction, pre-concentration) prototype compatible 
      with the capillary electrophoresis drug of abuse analyzer and suitable for 
      confirmatory analysis by mass spectrometry. The cotton swab from Salivette(R) oral 
      fluid collector was examined and integrated into the fully automated extractor 
      prototype. The recoveries for the automated extractor were between 18 and 20%, 
      with repeatabilities within 5-11% for 3,4-methylenedioxymethamphetamine (MDMA), 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxy-N-ethylamphetamine 
      (MDEA), cocaine (COC), and cocaethylene (COET). The developed extraction device 
      was easy to use even for unskilled persons, required minimal liquid handling, and 
      was applicable to use in field conditions.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Ruzicka, Martin
AU  - Ruzicka M
AD  - Tallinn University of Technology, School of Science, Department of Chemistry and 
      Biotechnology, Akadeemia tee 15, 12618, Tallinn, Estonia.
FAU - Kaljurand, Mihkel
AU  - Kaljurand M
AD  - Tallinn University of Technology, School of Science, Department of Chemistry and 
      Biotechnology, Akadeemia tee 15, 12618, Tallinn, Estonia.
FAU - Gorbatsova, Jelena
AU  - Gorbatsova J
AD  - Tallinn University of Technology, School of Science, Department of Chemistry and 
      Biotechnology, Akadeemia tee 15, 12618, Tallinn, Estonia.
FAU - Vaher, Merike
AU  - Vaher M
AD  - Tallinn University of Technology, School of Science, Department of Chemistry and 
      Biotechnology, Akadeemia tee 15, 12618, Tallinn, Estonia.
FAU - Mazina-Sinkar, Jekaterina
AU  - Mazina-Sinkar J
AD  - Tallinn University of Technology, School of Science, Department of Chemistry and 
      Biotechnology, Akadeemia tee 15, 12618, Tallinn, Estonia. Electronic address: 
      jekaterina.mazina@taltech.ee.
LA  - eng
PT  - Journal Article
DEP - 20220312
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Illicit Drugs)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Electrophoresis, Capillary/methods
MH  - Humans
MH  - *Illicit Drugs/analysis
MH  - Mass Spectrometry
MH  - *N-Methyl-3,4-methylenedioxyamphetamine
MH  - Substance Abuse Detection/methods
OTO - NOTNLM
OT  - Automated oral fluid extractor
OT  - Capillary electrophoresis
OT  - Drug analyzer
OT  - Fluorescence
OT  - Illegal drugs
OT  - Oral fluid
EDAT- 2022/03/18 06:00
MHDA- 2022/04/08 06:00
CRDT- 2022/03/17 20:12
PHST- 2021/12/27 00:00 [received]
PHST- 2022/03/08 00:00 [revised]
PHST- 2022/03/11 00:00 [accepted]
PHST- 2022/03/18 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/03/17 20:12 [entrez]
AID - S0039-9140(22)00185-0 [pii]
AID - 10.1016/j.talanta.2022.123389 [doi]
PST - ppublish
SO  - Talanta. 2022 Jun 1;243:123389. doi: 10.1016/j.talanta.2022.123389. Epub 2022 Mar 
      12.