PMID- 35300694
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20220406
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 18
IP  - 1
DP  - 2022 Mar 17
TI  - Molecular identification and functional characterization of IRF4 from common carp 
      (Cyprinus carpio. L) in immune response: a negative regulator in the IFN and 
      NF-kappaB signalling pathways.
PG  - 106
LID - 10.1186/s12917-022-03205-8 [doi]
LID - 106
AB  - BACKGROUND: The interferon (IFN) regulatory factors (IRFs) were originally 
      identified as transcription factors playing critical roles in the regulation of 
      IFN-related genes in the signal pathway. In mammals, IRF4 plays a vital role in 
      both the innate and adaptive immune system. This study aims to reveal the 
      molecular characterization, phylogenetic analysis, expression profiles and the 
      regulatory role in the IFN and NF-kappaB signalling pathways of IRF4 in common carp 
      (Cyprinus carpio. L) (abbreviation, ccIRF4). RESULTS: Here, ccIRF4 was identified 
      and characterized, it contained a DNA binding domain (DBD) which possess five 
      tryptophans and an IRF-associated domain (IAD). The predicted protein sequence of 
      the ccIRF4 showed higher identities with grass carp (Ctenopharyngodon idella) and 
      zebrafish (Danio rerio). Phylogenetic analysis suggested that ccIRF4 has the 
      closest relationship with zebrafish IRF4. Quantitative real-time PCR analysis 
      showed that ccIRF4 was constitutively expressed in all investigated tissues with 
      the highest expression level in the gonad. Polyinosinic:polycytidylic acid (poly 
      I:C) stimulation up-regulated the ccIRF4 expressions in the liver, spleen, head 
      kidney, skin, foregut and hindgut. Upon Aeromonas hydrophila injection, the 
      expression level of ccIRF4 was up-regulated in all tissues with the exception of 
      spleen. In addition, ccIRF4 was induced by lipopolysaccharide (LPS), 
      peptidoglycan (PGN) and Flagellin in head kidney leukocytes (HKLs). 
      Overexpression of the ccIRF4 gene in epithelioma papulosum cyprini cells (EPC) 
      down regulated the expressions of IFN-related genes and proinflammatory factors. 
      Dual-luciferase reporter assay revealed that ccIRF4 decreased the activation of 
      NF-kappaB through MyD88. CONCLUSIONS: These results indicate that ccIRF4 participates 
      in both antiviral and antibacterial immune response and negatively regulates the 
      IFN and NF-kappaB response. Overall, our study on ccIRF4 provides more new insights 
      into the innate immune system of common carp as well as a theoretical basis for 
      investigating the pathogenesis and prevention of fish disease.
CI  - (c) 2022. The Author(s).
FAU - Zhu, Yaoyao
AU  - Zhu Y
AD  - Key Laboratory of Tropical Marine Fishery Resources Protection and Utilization of 
      Hainan Province, College of Fisheries and Life Science, Hainan Tropical Ocean 
      University, No. 1 Yucai Road, Sanya, 572022, China. zhuyaoyao991@126.com.
AD  - Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery 
      Resources, Hainan Tropical Ocean University, Sanya, 572022, China. 
      zhuyaoyao991@126.com.
FAU - Yang, Guiwen
AU  - Yang G
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China. yanggw@sdnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220317
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Fish Proteins)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Aeromonas hydrophila
MH  - Animals
MH  - *Carps/genetics/metabolism
MH  - *Fish Diseases/genetics
MH  - Fish Proteins/genetics/metabolism
MH  - Immunity, Innate/genetics
MH  - Mammals
MH  - NF-kappa B/metabolism
MH  - Phylogeny
MH  - Signal Transduction
MH  - Zebrafish/metabolism
PMC - PMC8928632
OTO - NOTNLM
OT  - Common carp (Cyprinus carpio. L)
OT  - Interferon regulatory factor 4 (IRF4)
OT  - Poly (I:C), Aeromonas hydrophila
OT  - Signalling pathway
COIS- The authors declare that they have no competing interests.
EDAT- 2022/03/19 06:00
MHDA- 2022/04/07 06:00
PMCR- 2022/03/17
CRDT- 2022/03/18 05:35
PHST- 2022/01/06 00:00 [received]
PHST- 2022/03/07 00:00 [accepted]
PHST- 2022/03/18 05:35 [entrez]
PHST- 2022/03/19 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
PHST- 2022/03/17 00:00 [pmc-release]
AID - 10.1186/s12917-022-03205-8 [pii]
AID - 3205 [pii]
AID - 10.1186/s12917-022-03205-8 [doi]
PST - epublish
SO  - BMC Vet Res. 2022 Mar 17;18(1):106. doi: 10.1186/s12917-022-03205-8.