PMID- 35301062
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220509
IS  - 1873-1708 (Electronic)
IS  - 0890-6238 (Linking)
VI  - 109
DP  - 2022 Apr
TI  - Prenatal ethanol exposure induces dynamic changes of expression and activity of 
      hepatic cytochrome P450 isoforms in male rat offspring.
PG  - 101-108
LID - S0890-6238(22)00028-4 [pii]
LID - 10.1016/j.reprotox.2022.03.002 [doi]
AB  - This study aimed at determining the effect of prenatal ethanol exposure (PEE) on 
      the expression and activity of cytochrome P450 (CYP) isozymes at different life 
      stages of male rat offspring. Pregnant Wistar rats were administered with ethanol 
      (4 g/kg/d) intragastrically from gestational day (GD) 9-20. Male offspring's gene 
      and activity of CYP isozymes were analyzed on GD 20 (only expression), postnatal 
      day (PD) 84 and 196. Using aniline as probe, we compared the enzyme kinetics of 
      hepatic CYP2E1 between two groups. Expression of CYP isozymes was examined in rat 
      primary hepatocytes and human hepatic cell lines treated with ethanol or/and 
      glucocorticoid. Gene level of Cyp1a2, 2b1, 2d1, 2e1, 3a1 and aryl hydrocarbon 
      receptor were increased in PEE group on GD 20 and PD 84 and Cyp2e1 still 
      exhibited an increasing trend on PD 196 compared with the control. PEE inhibited 
      CYP2D1 and 2E1 activities in male offspring on PD 84. CYP activities in two 
      groups became the same level on PD 196. PEE induced an opposite change in gene 
      and protein level of hepatic CYP2E1 before and after birth. In consistent with 
      lower protein level, aniline metabolism in PEE was weaker in liver microsome. 
      Both single and combined use of ethanol or/and glucocorticoid increased CYPs 
      expression in vitro. In conclusion, PEE programmed a higher gene and lower 
      protein level of CYPs in male offspring, which dwindled with age. Impairment of 
      protein levels and enzyme activities of CYPs may affect individual metabolism of 
      endogenous and exogenous substances in early adulthood.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Sun, Xiaoxiang
AU  - Sun X
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan 430071, Hubei Province, People's Republic of China; Hubei Provincial Key 
      Laboratory of Developmentally Originated Diseases, Wuhan 430071, Hubei Province, 
      People's Republic of China.
FAU - He, Liang
AU  - He L
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan 430071, Hubei Province, People's Republic of China; Hubei Provincial Key 
      Laboratory of Developmentally Originated Diseases, Wuhan 430071, Hubei Province, 
      People's Republic of China.
FAU - Bi, Huichang
AU  - Bi H
AD  - School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, 
      Guangzhou Province, People's Republic of China.
FAU - Huang, Min
AU  - Huang M
AD  - School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, 
      Guangzhou Province, People's Republic of China.
FAU - Xiang, E
AU  - Xiang E
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan 430071, Hubei Province, People's Republic of China; Hubei Provincial Key 
      Laboratory of Developmentally Originated Diseases, Wuhan 430071, Hubei Province, 
      People's Republic of China.
FAU - Li, Xia
AU  - Li X
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan 430071, Hubei Province, People's Republic of China; Hubei Provincial Key 
      Laboratory of Developmentally Originated Diseases, Wuhan 430071, Hubei Province, 
      People's Republic of China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan 430071, Hubei Province, People's Republic of China; Hubei Provincial Key 
      Laboratory of Developmentally Originated Diseases, Wuhan 430071, Hubei Province, 
      People's Republic of China. Electronic address: wanghui19@whu.edu.cn.
FAU - Guo, Yu
AU  - Guo Y
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan 430071, Hubei Province, People's Republic of China; Hubei Provincial Key 
      Laboratory of Developmentally Originated Diseases, Wuhan 430071, Hubei Province, 
      People's Republic of China. Electronic address: guoy@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220314
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Protein Isoforms)
RN  - 3K9958V90M (Ethanol)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
SB  - IM
MH  - Animals
MH  - *Cytochrome P-450 Enzyme System/genetics/metabolism
MH  - Ethanol/toxicity
MH  - Female
MH  - *Liver/metabolism
MH  - Male
MH  - Pregnancy
MH  - Protein Isoforms/metabolism/pharmacology
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - CYP2E1
OT  - Cytochrome P450 (CYP)
OT  - Drug metabolism
OT  - Male rat offspring
OT  - Prenatal ethanol exposure
EDAT- 2022/03/19 06:00
MHDA- 2022/04/06 06:00
CRDT- 2022/03/18 05:39
PHST- 2021/06/27 00:00 [received]
PHST- 2022/03/05 00:00 [revised]
PHST- 2022/03/09 00:00 [accepted]
PHST- 2022/03/19 06:00 [pubmed]
PHST- 2022/04/06 06:00 [medline]
PHST- 2022/03/18 05:39 [entrez]
AID - S0890-6238(22)00028-4 [pii]
AID - 10.1016/j.reprotox.2022.03.002 [doi]
PST - ppublish
SO  - Reprod Toxicol. 2022 Apr;109:101-108. doi: 10.1016/j.reprotox.2022.03.002. Epub 
      2022 Mar 14.