PMID- 35301731
OWN - NLM
STAT- MEDLINE
DCOM- 20220708
LR  - 20220930
IS  - 1745-4514 (Electronic)
IS  - 0145-8884 (Linking)
VI  - 46
IP  - 7
DP  - 2022 Jul
TI  - Pinus koraiensis needle or cone extracts alleviate atopic dermatitis symptoms by 
      regulating immunity and suppressing inflammation in HaCaT cells and Nc/Nga mice.
PG  - e14135
LID - 10.1111/jfbc.14135 [doi]
AB  - Pinus koraiensis needles (PKN) and cones (PKC) have been shown to protect against 
      inflammation and pathogenic bacteria. We investigated the efficacies and action 
      mechanisms of topical applications of 1,3-butylene glycol (BG) extracts and oral 
      administration of their water extracts on atopic dermatitis (AD) symptoms. After 
      exposing HaCaT cells and Nc/Nga mice dorsal skins to 2,4-dinitrochlorobenzene 
      (DNCB) to induce atopic dermatitis models, they were topically applied BG 
      (AD-control), 30% PKNX, or 30% PKCX to the skin lesions and fed water extracts 
      (0.5%) in high-fat diets for 5 weeks. Normal-control mice had no DNCB exposure. 
      Serum immunoglobulin E (IgE), IL-4, and TNF-alpha levels and gene expressions of 
      TNF-alpha, IL-4, IL-6, and IFN-gamma in the dorsal skin and HaCaT cells were measured. 
      The AD-control mice elevated TNF-alpha and IL-6 mRNA levels in HaCaT cells. Both 
      extracts attenuated clinical AD symptoms in AD-induced Nc/Nga mice: PKNX improved 
      hemorrhage, erythema, and lichenification of dorsal skin better than PKCX while 
      both similarly alleviated erythema, edema, excoriation, and itching behavior. 
      PKCX reduced IgE contents and increased filaggrin mRNA expression better than 
      PKNX, but PKNX reduced lipid peroxides and mRNA levels of TNF-alpha and IL-4 in the 
      dorsal skin. In the histological analysis of the dorsal skin, the administration 
      of both extracts significantly decreased mast cell numbers, immune cell 
      infiltration, gaps between the epidermis and dermis, and abnormal cell and 
      nucleus shapes. In conclusion, both PKCX and PKNX treatment alleviated the 
      DNCB-induced clinical symptoms of AD by alleviating immune-related symptoms and 
      inflammation in partially different pathways. Therefore, PKNX and PKCX may be 
      effective for AD therapy. PRACTICAL APPLICATIONS: Atopic dermatitis (AD) is 
      related to an overly activated immune response, and it has steadily increased 
      last 3 decades. However, no optimal sustainable treatments are available. Pinus 
      koraiensis needles and cones extracts have been used for anti-inflammatory and 
      antimicrobial treatment. The present study demonstrated that their intake and 
      topical administration onto the AD lesion alleviated clinical AD symptoms 
      associated with reduced proinflammatory cytokines, mast cell numbers, and immune 
      cell infiltrates to maintain dermal structure with maintaining filaggrin 
      expression in AD-induced HaCaT cells and Nc/Nga mice. These results suggested 
      that Pinus koraiensis needles and cones extracts can be developed and applied as 
      beneficial alternative therapies for AD.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Ryuk, Jin Ah
AU  - Ryuk JA
AD  - Korea Institute of Oriental Medicine, Daejeon, South Korea.
FAU - Ko, Byoung-Seob
AU  - Ko BS
AD  - Korea Institute of Oriental Medicine, Daejeon, South Korea.
FAU - Moon, Na Rang
AU  - Moon NR
AD  - Food & Nutrition, Obesity/Diabetes Center, Hoseo University, Asan, South Korea.
FAU - Park, Sunmin
AU  - Park S
AUID- ORCID: 0000-0002-6092-8340
AD  - Food & Nutrition, Obesity/Diabetes Center, Hoseo University, Asan, South Korea.
AD  - Department of Bioconvergence System, Hoseo University, Asan, South Korea.
LA  - eng
GR  - 2019R1A2C1007203/The National Research Foundation of Korea/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220317
PL  - United States
TA  - J Food Biochem
JT  - Journal of food biochemistry
JID - 7706045
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 059QF0KO0R (Water)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - *Dermatitis, Atopic/chemically induced/drug therapy
MH  - Dinitrochlorobenzene/adverse effects
MH  - HaCaT Cells
MH  - Humans
MH  - Immunoglobulin E/adverse effects
MH  - Inflammation
MH  - Interleukin-4
MH  - Interleukin-6
MH  - Mice
MH  - *Pinus
MH  - RNA, Messenger
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - Water
OTO - NOTNLM
OT  - Pinus koraiensis
OT  - over-activation of immunity
OT  - pine cones
OT  - pine needles
OT  - proinflammatory cytokines
EDAT- 2022/03/19 06:00
MHDA- 2022/07/09 06:00
CRDT- 2022/03/18 05:44
PHST- 2022/01/30 00:00 [revised]
PHST- 2021/09/09 00:00 [received]
PHST- 2022/02/24 00:00 [accepted]
PHST- 2022/03/19 06:00 [pubmed]
PHST- 2022/07/09 06:00 [medline]
PHST- 2022/03/18 05:44 [entrez]
AID - 10.1111/jfbc.14135 [doi]
PST - ppublish
SO  - J Food Biochem. 2022 Jul;46(7):e14135. doi: 10.1111/jfbc.14135. Epub 2022 Mar 17.