PMID- 35303681
OWN - NLM
STAT- MEDLINE
DCOM- 20220411
LR  - 20220531
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 604
DP  - 2022 May 14
TI  - A quantitative proteomic analysis reveals the potential roles of PRDX3 in neurite 
      outgrowth in N2a-APPswe cells.
PG  - 144-150
LID - S0006-291X(22)00346-1 [pii]
LID - 10.1016/j.bbrc.2022.03.021 [doi]
AB  - Alzheimer's disease (AD) is characterized by amyloid plaques and neurofibrillary 
      tangles accompanied by progressive neurite loss. Mitochondria play pivotal roles 
      in AD development. PRDX3 is a mitochondrial peroxide reductase critical for 
      H(2)O(2) scavenging and signal transduction. In this study, we found that PRDX3 
      knockdown (KD) in the N2a-APPswe cell line promoted retinoic acid (RA)-induced 
      neurite outgrowth but did not reduce the viability of cells damaged by tert-butyl 
      hydroperoxide (TBHP). We found that knocking down PRDX3 expression induced 
      dysregulation of more than one hundred proteins, as determined by tandem mass tag 
      (TMT)-labeled proteomics. A Gene Ontology (GO) analysis revealed that the 
      dysregulated proteins were enriched in protein localization to the plasma 
      membrane, the lipid catabolic process, and intermediate filament cytoskeleton 
      organization. A STRING analysis showed close protein-protein interactions among 
      dysregulated proteins. The expression of Annexin A1 (ANXA1), serine 
      (Ser)-/threonine (Thr)-protein phosphatase 2A catalytic subunit alpha isoform 
      (PP2A) and glutathione S-transferase Mu 2 (GSTM2) was significantly upregulated 
      in PRDX3-KD N2a-APPswe cell lines, as verified by western blotting. Our study 
      revealed, for the first time, that PRDX3 may play important roles in neurite 
      outgrowth and AD development.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Xu, Benhong
AU  - Xu B
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China. Electronic address: xubenhong0916@163.com.
FAU - Gao, Chuanyue
AU  - Gao C
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China.
FAU - Zhang, Huan
AU  - Zhang H
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China.
FAU - Huang, Xinfeng
AU  - Huang X
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China.
FAU - Yang, Xifei
AU  - Yang X
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China.
FAU - Yang, Chen
AU  - Yang C
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China.
FAU - Wu, Desheng
AU  - Wu D
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China.
FAU - Liu, Jianjun
AU  - Liu J
AD  - Shenzhen Key Laboratory of Modern Toxicology, Shenzhen Medical Key Discipline of 
      Health Toxicology (2020-2024), Shenzhen Center for Disease Control and 
      Prevention, China. Electronic address: junii8@126.com.
LA  - eng
PT  - Journal Article
DEP - 20220305
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.15 (PRDX3 protein, human)
RN  - EC 1.11.1.15 (Peroxiredoxin III)
SB  - IM
MH  - *Alzheimer Disease/metabolism
MH  - Cell Line, Tumor
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Neurites/metabolism
MH  - *Neuronal Outgrowth/genetics
MH  - *Peroxiredoxin III/genetics/metabolism
MH  - Proteomics
OTO - NOTNLM
OT  - Mitochondria
OT  - Neurite outgrowth
OT  - PRDX3
OT  - Proteomic
COIS- Declaration of competing interest The authors declare that there was no potential 
      conflict of interest existed.
EDAT- 2022/03/19 06:00
MHDA- 2022/04/12 06:00
CRDT- 2022/03/18 20:13
PHST- 2022/02/17 00:00 [received]
PHST- 2022/02/18 00:00 [revised]
PHST- 2022/03/03 00:00 [accepted]
PHST- 2022/03/19 06:00 [pubmed]
PHST- 2022/04/12 06:00 [medline]
PHST- 2022/03/18 20:13 [entrez]
AID - S0006-291X(22)00346-1 [pii]
AID - 10.1016/j.bbrc.2022.03.021 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2022 May 14;604:144-150. doi: 
      10.1016/j.bbrc.2022.03.021. Epub 2022 Mar 5.