PMID- 35303795
OWN - NLM
STAT- MEDLINE
DCOM- 20220429
LR  - 20220531
IS  - 1528-3658 (Electronic)
IS  - 1076-1551 (Print)
IS  - 1076-1551 (Linking)
VI  - 28
IP  - 1
DP  - 2022 Mar 18
TI  - Exosomal microRNA-140-3p from human umbilical cord mesenchymal stem cells 
      attenuates joint injury of rats with rheumatoid arthritis by silencing SGK1.
PG  - 36
LID - 10.1186/s10020-022-00451-2 [doi]
LID - 36
AB  - OBJECTIVE: Over the years, microRNAs (miRNAs) have been involved in the 
      pathogenesis of rheumatoid arthritis (RA). We aim to investigate the role of 
      human umbilical cord mesenchymal stem cells (HUCMSCs)-derived exosomal miR-140-3p 
      in RA development. METHODS: Exosomes(exo) were isolated from human umbilical 
      cord-derived mesenchymal stem cells (HUCMSCs), and this isolation was followed by 
      the transfer of miR-140-3p. RA rat models were constructed by collagen II 
      adjuvant and respectively treated with HUCMSCs-exo or HUCMSCs-exo carrying 
      miR-140-3p mimic/inhibitor, and expression of miR-140-3p and serum- and 
      glucocorticoid-inducible kinase 1 (SGK1) was assessed. Then, RA score and 
      inflammation scoring, fibrosis degree and apoptosis, serum inflammatory response 
      and oxidative stress in joint tissues were determined. The RA synovial 
      fibroblasts (RASFs) were extracted from rats and identified. Conducted with 
      relative treatment, the migration, proliferation and apoptosis in RASFs were 
      determined. RESULTS: MiR-140-3p was decreased while SGK1 was increased in RA 
      rats. HUCMSCs-exo or upregulated exosomal miR-140-3p improved pathological 
      changes and suppressed inflammation, oxidative stress and fibrosis in RA rats, 
      and also constrained and RASF growth. Overexpression of SGK1 reversed the 
      inhibition of RASF growth caused by overexpression of miR-140-3p. CONCLUSION: 
      Upregulated exosomal miR-140-3p attenuated joint injury of RA rats by silencing 
      SGK1. This research provided further understanding of the role of exosomal 
      miR-140-3p in RA development.
CI  - (c) 2022. The Author(s).
FAU - Huang, Yijiang
AU  - Huang Y
AD  - Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, 109 West College Road, Wenzhou, 325000, 
      China.
AD  - Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325000, China.
FAU - Chen, Liang
AU  - Chen L
AD  - Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, 109 West College Road, Wenzhou, 325000, 
      China.
AD  - Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325000, China.
FAU - Chen, Daosen
AU  - Chen D
AD  - Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, 109 West College Road, Wenzhou, 325000, 
      China.
AD  - Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325000, China.
FAU - Fan, Pei
AU  - Fan P
AD  - Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, 109 West College Road, Wenzhou, 325000, 
      China.
AD  - Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325000, China.
FAU - Yu, Huachen
AU  - Yu H
AUID- ORCID: 0000-0003-2734-4295
AD  - Department of Orthopaedic, The Second Affiliated Hospital and Yuying Children's 
      Hospital of Wenzhou Medical University, 109 West College Road, Wenzhou, 325000, 
      China. Yuhuachen079@163.com.
AD  - Key Laboratory of Orthopaedics of Zhejiang Province, Wenzhou, 325000, China. 
      Yuhuachen079@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220318
PL  - England
TA  - Mol Med
JT  - Molecular medicine (Cambridge, Mass.)
JID - 9501023
RN  - 0 (MIRN140 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn140 microRNA, human)
SB  - IM
MH  - Animals
MH  - *Arthritis, Rheumatoid/genetics/therapy
MH  - Cell Proliferation
MH  - *Exosomes
MH  - Fibrosis
MH  - Humans
MH  - Inflammation/pathology
MH  - *Mesenchymal Stem Cells
MH  - *MicroRNAs/genetics
MH  - Rats
MH  - Umbilical Cord/pathology
PMC - PMC8932126
OTO - NOTNLM
OT  - Exosome
OT  - Joint injury
OT  - MicroRNA-140-3p
OT  - Rat
OT  - Rheumatoid arthritis
OT  - Serum-and glucocorticoid-inducible kinase 1
COIS- The authors declare no competing interests.
EDAT- 2022/03/20 06:00
MHDA- 2022/04/30 06:00
PMCR- 2022/03/18
CRDT- 2022/03/19 05:25
PHST- 2021/04/08 00:00 [received]
PHST- 2022/02/04 00:00 [accepted]
PHST- 2022/03/19 05:25 [entrez]
PHST- 2022/03/20 06:00 [pubmed]
PHST- 2022/04/30 06:00 [medline]
PHST- 2022/03/18 00:00 [pmc-release]
AID - 10.1186/s10020-022-00451-2 [pii]
AID - 451 [pii]
AID - 10.1186/s10020-022-00451-2 [doi]
PST - epublish
SO  - Mol Med. 2022 Mar 18;28(1):36. doi: 10.1186/s10020-022-00451-2.