PMID- 35307459
OWN - NLM
STAT- MEDLINE
DCOM- 20220414
LR  - 20220414
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 206
DP  - 2022 May 1
TI  - Purification, characterization and partial biological activities of 
      exopolysaccharide produced by Saccharomyces cerevisiae Y3.
PG  - 777-787
LID - S0141-8130(22)00553-0 [pii]
LID - 10.1016/j.ijbiomac.2022.03.083 [doi]
AB  - In this study, the exopolysaccharide (EPS) from Saccharomyces cerevisiae Y3 was 
      extracted and purified, the preliminary structure characteristics and biological 
      activities was investigated. The S. cerevisiae Y3 EPS was obtained by ethanol 
      precipitation and gel filtration chromatography. M(W) of purified Y3 EPS was 
      93,477 Da. High-performance liquid chromatography (HPLC), fourier transform 
      infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and 
      methylation analyses showed that the EPS was a heteropolysaccharide, which 
      composed of 1-alpha-D mannose (39.8%), 1,2-alpha-D mannose (19.6%), 1,6-alpha-D linked 
      mannose (10.4%), 1,3,6-beta-D glucose (27.5%), and 1-beta-D linked glucose (1.9%). 
      Scanning electron microscope (SEM) and atomic force microscope (AFM) further 
      revealed smooth and dense sheet-like structure with reticular configuration. The 
      Congo red test and X-ray diffraction (XRD) reflected an irregular coil 
      conformation and non-crystalline amorphous nature. The EPS exhibited good 
      hydrophilicity, thermal stability and antioxidation ability for DPPH radicals, 
      hydroxyl radicals and NO(2-), as well as good prebiotic properties. These results 
      indicated that Y3 EPS could be explored as a promising functional adjunct for 
      application in probiotics and antioxidation.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Liu, Lina
AU  - Liu L
AD  - Engineering Research Center of Agricultural Microbiology Technology, Ministry of 
      Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of 
      Microbiology, College of Heilongjiang Province, School of Life Sciences, 
      Heilongjiang University, Harbin 150080, PR China.
FAU - Xu, Jiaju
AU  - Xu J
AD  - Engineering Research Center of Agricultural Microbiology Technology, Ministry of 
      Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of 
      Microbiology, College of Heilongjiang Province, School of Life Sciences, 
      Heilongjiang University, Harbin 150080, PR China.
FAU - Na, Ruiying
AU  - Na R
AD  - Engineering Research Center of Agricultural Microbiology Technology, Ministry of 
      Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of 
      Microbiology, College of Heilongjiang Province, School of Life Sciences, 
      Heilongjiang University, Harbin 150080, PR China.
FAU - Du, Renpeng
AU  - Du R
AD  - Engineering Research Center of Agricultural Microbiology Technology, Ministry of 
      Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of 
      Microbiology, College of Heilongjiang Province, School of Life Sciences, 
      Heilongjiang University, Harbin 150080, PR China.
FAU - Ping, Wenxiang
AU  - Ping W
AD  - Engineering Research Center of Agricultural Microbiology Technology, Ministry of 
      Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of 
      Microbiology, College of Heilongjiang Province, School of Life Sciences, 
      Heilongjiang University, Harbin 150080, PR China.
FAU - Ge, Jingping
AU  - Ge J
AD  - Engineering Research Center of Agricultural Microbiology Technology, Ministry of 
      Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of 
      Microbiology, College of Heilongjiang Province, School of Life Sciences, 
      Heilongjiang University, Harbin 150080, PR China.
FAU - Zhao, Dan
AU  - Zhao D
AD  - Engineering Research Center of Agricultural Microbiology Technology, Ministry of 
      Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of 
      Microbiology, College of Heilongjiang Province, School of Life Sciences, 
      Heilongjiang University, Harbin 150080, PR China; Guangxi Key Laboratory for 
      Polysaccharide Materials and Modifications, School of Marine Sciences and 
      Biotechnology, Guangxi University for Nationalities, Nanning 530008, PR China. 
      Electronic address: zhaodan4u@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220318
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - 0 (Polysaccharides, Bacterial)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Antioxidants/chemistry
MH  - Mannose
MH  - Molecular Weight
MH  - *Polysaccharides, Bacterial/chemistry
MH  - *Saccharomyces cerevisiae
MH  - Spectroscopy, Fourier Transform Infrared
OTO - NOTNLM
OT  - Exopolysaccharides
OT  - Identification
OT  - Purification
OT  - Saccharomyces cerevisiae
OT  - Structure
EDAT- 2022/03/22 06:00
MHDA- 2022/04/15 06:00
CRDT- 2022/03/21 05:34
PHST- 2021/10/18 00:00 [received]
PHST- 2022/03/13 00:00 [revised]
PHST- 2022/03/14 00:00 [accepted]
PHST- 2022/03/22 06:00 [pubmed]
PHST- 2022/04/15 06:00 [medline]
PHST- 2022/03/21 05:34 [entrez]
AID - S0141-8130(22)00553-0 [pii]
AID - 10.1016/j.ijbiomac.2022.03.083 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2022 May 1;206:777-787. doi: 10.1016/j.ijbiomac.2022.03.083. 
      Epub 2022 Mar 18.