PMID- 35308329
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220322
IS  - 2476-4108 (Print)
IS  - 2476-3772 (Electronic)
IS  - 2476-3772 (Linking)
VI  - 20
IP  - 1
DP  - 2022 Jan
TI  - The effect of dexamethasone on uterine receptivity, mediated by the ERK1/2-mTOR 
      pathway, and the implantation window: An experimental study.
PG  - 47-58
LID - 10.18502/ijrm.v20i1.10408 [doi]
AB  - BACKGROUND: The role of glucocorticoids in implantation has been demonstrated. 
      OBJECTIVE: This study aimed to evaluate the effect of dexamethasone on 
      endometrial receptivity. MATERIALS AND METHODS: In this experimental study, 40 
      BALB/c female mice aged eight wk old weighing approximately 25.0 +/- 1.4 gr were 
      used. The mice were divided into four groups (n = 10/each) of control, 
      dexamethasone (100 mug/kg, intraperitoneal injection), mammalian target of 
      rapamycin (mTOR) inhibitor (PP242) (30 mg/kg, intraperitoneal injection), and 
      dexamethasone and PP242. The endometrial epithelium of the mouse was separated to 
      measure messenger RNA expression of heart and neural crest derivatives-expressed 
      protein 2 (HAND2), Msh homeobox 1 (Msx-1), heparin binding epidermal growth 
      factor (HB-EGF), microRNA (miRNA) Let-7a, miRNA-145 and miRNA-451, using 
      real-time polymerase chain reaction. Also, protein expression of mammalian mTOR 
      and eukaryotic translation initiation factor 4E-binding protein1 (4E-BP1) was 
      measured using western blot. RESULTS: The results revealed that the expression of 
      Msx-1, HAND2, HB-EGF, miRNA-451, and miRNA-Let-7a was significantly decreased in 
      the endometrium in the dexamethasone group compared to the control, while the 
      expression of miRNA-145 in the endometrium was up-regulated. Additionally, the 
      administration of PP242, known as an inhibitor of mTOR, was associated with 
      significantly reduced expression of Msx-1, HAND2, HB-EGF, miRNA-451, and 
      miRNA-Let-7a, while PP242 induced messenger RNA expression of miRNA-145. 
      CONCLUSION: It appears that dexamethasone can diminish uterine receptivity during 
      the implantation period, at least to some extent, through the alteration of 
      particular genes that impact endometrial receptivity. Furthermore, the mTOR 
      pathway seemingly showed an essential role in endometrial receptivity.
CI  - Copyright (c) 2022 Niknafs et al.
FAU - Niknafs, Behrooz
AU  - Niknafs B
AD  - Immunology Research Center, Faculty of Medicine, Tabriz University of Medical 
      Sciences, Tabriz, Iran.
FAU - Shokrzadeh, Naser
AU  - Shokrzadeh N
AD  - Infertility and Reproductive Health Research Center, Health Research Institute, 
      Babol University of Medical Sciences, Babol, Iran.
FAU - Reza Alivand, Mohammad
AU  - Reza Alivand M
AD  - Department of Genetics, Faculty of Medicine, Tabriz University of Medical 
      Sciences, Tabriz, Iran.
FAU - Bakhtiar Hesam Shariati, Mohammad
AU  - Bakhtiar Hesam Shariati M
AUID- ORCID: 0000-0002-2000-5197
AD  - Department of Anatomical Sciences and Histology, School of Medicine, Kurdistan 
      University of Medical Sciences, Sanandaj, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220218
PL  - Iran
TA  - Int J Reprod Biomed
JT  - International journal of reproductive biomedicine
JID - 101679102
PMC - PMC8902795
OTO - NOTNLM
OT  - HB-EGF
OT  - MSX-1
OT  - mTOR, Mouse.
OT  - Dexamethasone
OT  - Endometrial receptivity
COIS- The authors declare that there is no conflict of interest that prejudices the 
      impartiality of this scientific work.
EDAT- 2022/03/22 06:00
MHDA- 2022/03/22 06:01
PMCR- 2022/02/18
CRDT- 2022/03/21 08:38
PHST- 2020/06/10 00:00 [received]
PHST- 2020/12/22 00:00 [revised]
PHST- 2021/02/23 00:00 [accepted]
PHST- 2022/03/21 08:38 [entrez]
PHST- 2022/03/22 06:00 [pubmed]
PHST- 2022/03/22 06:01 [medline]
PHST- 2022/02/18 00:00 [pmc-release]
AID - 10.18502/ijrm.v20i1.10408 [doi]
PST - epublish
SO  - Int J Reprod Biomed. 2022 Feb 18;20(1):47-58. doi: 10.18502/ijrm.v20i1.10408. 
      eCollection 2022 Jan.