PMID- 35314294
OWN - NLM
STAT- MEDLINE
DCOM- 20220420
LR  - 20220420
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 163
DP  - 2022 May
TI  - Deoxynivalenol induces apoptosis and inflammation in the liver: Analysis using 
      precision-cut liver slices.
PG  - 112930
LID - S0278-6915(22)00128-4 [pii]
LID - 10.1016/j.fct.2022.112930 [doi]
AB  - Deoxynivalenol (DON) is one of the most common mycotoxins in cereals and their 
      by-products. Its adverse effects on animal and human health have been extensively 
      studied in the intestine, but little attention has been paid to another target 
      organ for mycotoxins, the liver that is potentially exposed after intestinal 
      absorption and enterohepatic circulation. To assess DON's toxicity in an ex vivo 
      model structurally and physiologically closer to the whole liver, we developed a 
      pig precision-cut liver slices (PCLS) model. PCLS contain all cell types and 
      maintain intercellular and cell-matrix interactions, among other architectural 
      features of the liver. The human HepG2 cell line was used for comparison. We 
      observed that after a short exposure, DON reduced the cell viability of HepG2 
      cells and induced the expression of genes involved in apoptosis, inflammation and 
      oxidative stress. When PCLS were exposed to DON, damage to the tissues was 
      observed, with no changes in markers of liver function or injury. Exposure to the 
      toxin also triggered liver inflammation and apoptosis, effects already observed 
      in pigs fed DON-contaminated diets. Overall, these data demonstrate that DON had 
      toxic effects on a liver cell line and on whole liver tissue, consistent with the 
      effect observed during in vivo exposure. They also indicate that pig PCLS is a 
      relevant and sensitive model to investigate the liver toxicity of food 
      contaminants.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Hasuda, Amanda Lopes
AU  - Hasuda AL
AD  - Laboratory of Animal Pathology, Universidade Estadual de Londrina, P.O. Box 
      10.011, Londrina, PR, 86057-970, Brazil; Toxalim (Research Centre in Food 
      Toxicology), University of Toulouse, INRAE, ENVT, INP-Purpan, UPS, 31027, 
      Toulouse, France. Electronic address: amanda.lopeshasuda@uel.br.
FAU - Person, Elodie
AU  - Person E
AD  - Toxalim (Research Centre in Food Toxicology), University of Toulouse, INRAE, 
      ENVT, INP-Purpan, UPS, 31027, Toulouse, France. Electronic address: 
      elodie.person@inrae.fr.
FAU - Khoshal, Abdullah Khan
AU  - Khoshal AK
AD  - Toxalim (Research Centre in Food Toxicology), University of Toulouse, INRAE, 
      ENVT, INP-Purpan, UPS, 31027, Toulouse, France. Electronic address: 
      a.khoshal@outlook.com.
FAU - Bruel, Sandrine
AU  - Bruel S
AD  - Toxalim (Research Centre in Food Toxicology), University of Toulouse, INRAE, 
      ENVT, INP-Purpan, UPS, 31027, Toulouse, France. Electronic address: 
      sandrine.bruel@inrae.fr.
FAU - Puel, Sylvie
AU  - Puel S
AD  - Toxalim (Research Centre in Food Toxicology), University of Toulouse, INRAE, 
      ENVT, INP-Purpan, UPS, 31027, Toulouse, France. Electronic address: 
      sylvie.puel@inrae.fr.
FAU - Oswald, Isabelle P
AU  - Oswald IP
AD  - Toxalim (Research Centre in Food Toxicology), University of Toulouse, INRAE, 
      ENVT, INP-Purpan, UPS, 31027, Toulouse, France. Electronic address: 
      isabelle.oswald@inrae.fr.
FAU - Bracarense, Ana Paula F R L
AU  - Bracarense APFRL
AD  - Laboratory of Animal Pathology, Universidade Estadual de Londrina, P.O. Box 
      10.011, Londrina, PR, 86057-970, Brazil. Electronic address: anapaula@uel.br.
FAU - Pinton, Philippe
AU  - Pinton P
AD  - Toxalim (Research Centre in Food Toxicology), University of Toulouse, INRAE, 
      ENVT, INP-Purpan, UPS, 31027, Toulouse, France. Electronic address: 
      philippe.pinton@inrae.fr.
LA  - eng
PT  - Journal Article
DEP - 20220318
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Mycotoxins)
RN  - 0 (Trichothecenes)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - *Food Contamination/analysis
MH  - Inflammation/chemically induced/metabolism
MH  - Liver/metabolism
MH  - *Mycotoxins/analysis
MH  - Swine
MH  - Trichothecenes
OTO - NOTNLM
OT  - Apoptosis
OT  - Cell death
OT  - Explants
OT  - Histology
OT  - Inflammation
OT  - Liver
OT  - Mycotoxins
EDAT- 2022/03/23 06:00
MHDA- 2022/04/21 06:00
CRDT- 2022/03/22 05:34
PHST- 2022/01/27 00:00 [received]
PHST- 2022/02/28 00:00 [revised]
PHST- 2022/03/16 00:00 [accepted]
PHST- 2022/03/23 06:00 [pubmed]
PHST- 2022/04/21 06:00 [medline]
PHST- 2022/03/22 05:34 [entrez]
AID - S0278-6915(22)00128-4 [pii]
AID - 10.1016/j.fct.2022.112930 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2022 May;163:112930. doi: 10.1016/j.fct.2022.112930. Epub 2022 
      Mar 18.