PMID- 35315049
OWN - NLM
STAT- MEDLINE
DCOM- 20220323
LR  - 20220323
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 39
IP  - 3
DP  - 2022 Mar 10
TI  - [Analysis of loss of heterozygosity at HLA loci in a patient with leukemia].
PG  - 338-342
LID - 10.3760/cma.j.cn511374-20210310-00209 [doi]
AB  - OBJECTIVE: To detect loss of heterozygosity (LOH) at human leukocyte antigen 
      (HLA) loci in a Chinese patient with leukemia after haploidentical hematopoietic 
      stem cell transplantation. METHODS: HLA genotyping was carried out on peripheral 
      blood, hair follicle and buccal swab samples derived from the patient after the 
      transplantation as well as peripheral blood samples from his parents by using 
      PCR-sequence specific oligonucleotide probe method and PCR-sequence based typing 
      method. Short tandem repeat (STR) loci were detected by using a 23 site STR assay 
      kit and a self-developed 6 STR loci assay for the HLA regions. RESULTS: After the 
      transplantation, the HLA genotype of the peripheral blood sample of the patient 
      was identical to his father. The patient was HLA-A*02:01,24:02, C*03:03,03:04, 
      B*13:01,15:01, DRB1*08:03,12:02, DQB1*03:01,06:01 for his hair follicle specimen. 
      However, homozygosity of the HLA loci was found in his buccal swab sample. Only 
      the HLA-A*24:02-C*03:03-B*15:01-DRB1*08:03-DQB1*06:01 haplotype from his father's 
      was present, while the HLA-A*02:01-C*03:04-B*13:01-DRB1*12:02-DQB1*03:01 
      haplotype from his mother was lost. After the transplantation, the alleles of the 
      23 STR sites in the patient's peripheral blood sample were consistent to his 
      father, with no allelic loss detected in his buccal swab sample. However, at 
      least 4 STR loci in the HLA region were lost in his buccal swab sample. 
      CONCLUSION: LOH at the HLA loci has been detected in the buccal swab sample of a 
      patient with leukemia who received haploidentical hematopoietic stem cell 
      transplantation.
FAU - Wang, Wei
AU  - Wang W
AD  - Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of 
      Zhejiang Province, Hangzhou, Zhejiang 310052, China. zfm00@hotmail.com.
FAU - Wang, Fang
AU  - Wang F
FAU - Dong, Lina
AU  - Dong L
FAU - Chen, Nanying
AU  - Chen N
FAU - He, Yizhen
AU  - He Y
FAU - Zhang, Wei
AU  - Zhang W
FAU - He, Ji
AU  - He J
FAU - Zhu, Faming
AU  - Zhu F
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - *HLA Antigens/genetics
MH  - HLA-A Antigens/genetics
MH  - Histocompatibility Antigens Class I/genetics
MH  - Humans
MH  - *Leukemia/genetics
MH  - Loss of Heterozygosity
EDAT- 2022/03/23 06:00
MHDA- 2022/03/24 06:00
CRDT- 2022/03/22 05:38
PHST- 2022/03/22 05:38 [entrez]
PHST- 2022/03/23 06:00 [pubmed]
PHST- 2022/03/24 06:00 [medline]
AID - 940639066 [pii]
AID - 10.3760/cma.j.cn511374-20210310-00209 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2022 Mar 10;39(3):338-342. doi: 
      10.3760/cma.j.cn511374-20210310-00209.