PMID- 35315493
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220407
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 25
IP  - 5
DP  - 2022 May
TI  - Taurine attenuates ER stress‑associated apoptosis and catabolism in nucleus 
      pulposus cells.
LID - 172 [pii]
LID - 10.3892/mmr.2022.12688 [doi]
AB  - Nucleus pulposus (NP) apoptosis and subsequent excessive degradation of the 
      extracellular matrix (ECM) are key pathological characteristics of intervertebral 
      disc degeneration (IDD). The present study aims to examine the signaling 
      processes underlying the effects of taurine on IDD, with specific focus on 
      endoplasmic reticulum (ER) stress‑mediated apoptosis and ECM degradation, in NP 
      cells. To clarify the role of taurine in IDD, NP cells were treated with various 
      concentrations of taurine and IL‑1beta or thapsigargin (TG). Cell Counting Kit‑8, 
      western blotting, TUNEL, immunofluorescence assays and reverse 
      transcription‑quantitative PCR were applied to measure cell viability, the 
      expression of ER stress‑associated proteins (GRP78, CHOP and caspase‑12), 
      apoptosis and the levels of metabolic factors associated with ECM (MMP‑1, 3, 9, 
      ADAMTS‑4, 5 and collagen II), respectively. Taurine was found to attenuate ER 
      stress and prevent apoptosis in NP cells induced by IL‑1beta treatment. 
      Additionally, taurine significantly decreased the expression of ER 
      stress‑activated glucose regulatory protein 78, C/EBP homologous protein and 
      caspase‑12. TUNEL results revealed that taurine decreased the number of apoptotic 
      TG‑treated NP cells. TG‑treated NP cells also exhibited characteristics of 
      increased ECM degradation, supported by observations of increased MMP‑1, MMP‑3, 
      MMP‑9 and A disintegrin and metalloproteinase with thrombospondin motifs 
      (ADAMTS)‑4 and ADAMTS‑5 expression in addition to decreased collagen‑II 
      expression. However, taurine treatment significantly reversed all indicators of 
      excessive ECM catabolism aforementioned. These data suggest that taurine can 
      mediate protection against apoptosis and ECM degradation in NP cells by 
      inhibiting ER stress, implicating therapeutic potential for the treatment of IDD.
FAU - Yang, Liuxie
AU  - Yang L
AD  - Department of Orthopedics, Shanghai Jing'an District Zhabei Central Hospital, 
      Shanghai 200040, P.R. China.
FAU - Li, Zhenhuan
AU  - Li Z
AD  - Department of Orthopedics, Shanghai Jing'an District Zhabei Central Hospital, 
      Shanghai 200040, P.R. China.
FAU - Ouyang, Yueping
AU  - Ouyang Y
AD  - Department of Orthopedics, Shanghai Changzheng Hospital, Second Military Medical 
      University, Shanghai 200003, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20220322
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 1EQV5MLY3D (Taurine)
SB  - IM
MH  - Apoptosis
MH  - Endoplasmic Reticulum Stress
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - *Intervertebral Disc Degeneration/metabolism
MH  - *Nucleus Pulposus/metabolism
MH  - Taurine/metabolism
PMC - PMC8971911
OTO - NOTNLM
OT  - apoptosis
OT  - catabolism
OT  - endoplasmic reticulum stress
OT  - intervertebral disc degeneration
OT  - taurine
COIS- The authors declare that they have no competing interests.
EDAT- 2022/03/23 06:00
MHDA- 2022/04/08 06:00
PMCR- 2022/03/18
CRDT- 2022/03/22 08:59
PHST- 2021/11/29 00:00 [received]
PHST- 2022/02/10 00:00 [accepted]
PHST- 2022/03/22 08:59 [entrez]
PHST- 2022/03/23 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/03/18 00:00 [pmc-release]
AID - 172 [pii]
AID - MMR-25-05-12688 [pii]
AID - 10.3892/mmr.2022.12688 [doi]
PST - ppublish
SO  - Mol Med Rep. 2022 May;25(5):172. doi: 10.3892/mmr.2022.12688. Epub 2022 Mar 22.