PMID- 35318648
OWN - NLM
STAT- MEDLINE
DCOM- 20220523
LR  - 20230722
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 166
IP  - 2
DP  - 2022 Jun
TI  - Tumour-associated antigenic peptides are present in the HLA class I ligandome of 
      cancer cell line derived extracellular vesicles.
PG  - 249-264
LID - 10.1111/imm.13471 [doi]
AB  - The recent success of monoclonal antibody checkpoint inhibitor therapies that 
      enhance the ability of CD8(+) T cells to detect cancer-related antigenic peptides 
      has refocused the need to fully understand the repertoire of peptides being 
      presented to the immune system. Whilst the peptide ligandome presented by cell 
      surface human leucocyte antigen class I (HLA-I) molecules on cancer cells has 
      been studied extensively, the ligandome of extracellular vesicles (EVs) remains 
      poorly defined. Here, we report the HLA-I ligandome of both the cell surface and 
      EVs from eight breast cancer cell lines (MCF7, MDA-MB-231, MDA-MB-361, 
      MDA-MB-415, MDA-MB-453, HCC 1806, HCC 1395, and HCC 1954), and additionally the 
      melanoma cell line ESTDAB-056 and the multiple myeloma line RPMI 8226. Utilizing 
      HLA-I immunoisolation and mass spectrometry, we detected a total of 6574 peptides 
      from the cell surface and 2461 peptides from the EVs of the cell lines studied. 
      Within the EV HLA-I ligandome, we identified 150 peptides derived from tumour 
      associated antigenic proteins, of which 19 peptides have been shown to elicit 
      T-cell responses in previous studies. Our data thus show the prevalence of 
      clinically relevant tumour-associated antigenic peptides in the HLA-I ligandome 
      presented on EV.
CI  - (c) 2022 The Authors. Immunology published by John Wiley & Sons Ltd.
FAU - Kumar, Pankaj
AU  - Kumar P
AD  - School of Medicine, University of St Andrews, St Andrews, UK.
FAU - Boyne, Caitlin
AU  - Boyne C
AD  - School of Medicine, University of St Andrews, St Andrews, UK.
FAU - Brown, Sydney
AU  - Brown S
AD  - School of Medicine, University of St Andrews, St Andrews, UK.
FAU - Qureshi, Ayesha
AU  - Qureshi A
AUID- ORCID: 0000-0003-0910-153X
AD  - School of Medicine, University of St Andrews, St Andrews, UK.
FAU - Thorpe, Peter
AU  - Thorpe P
AD  - School of Medicine, University of St Andrews, St Andrews, UK.
FAU - Synowsky, Silvia A
AU  - Synowsky SA
AD  - School of Biology, University of St Andrews, St Andrews, UK.
AD  - Biomedical Sciences Research Complex, University of St Andrews, St Andrews, UK.
FAU - Shirran, Sally
AU  - Shirran S
AD  - School of Biology, University of St Andrews, St Andrews, UK.
AD  - Biomedical Sciences Research Complex, University of St Andrews, St Andrews, UK.
FAU - Powis, Simon J
AU  - Powis SJ
AUID- ORCID: 0000-0003-4218-2984
AD  - School of Medicine, University of St Andrews, St Andrews, UK.
AD  - Biomedical Sciences Research Complex, University of St Andrews, St Andrews, UK.
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - 105621/Z/14/Z/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220420
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
SB  - IM
MH  - Antigens, Neoplasm
MH  - CD8-Positive T-Lymphocytes
MH  - *Carcinoma, Hepatocellular/metabolism
MH  - Cell Line
MH  - *Extracellular Vesicles
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - Ligands
MH  - *Liver Neoplasms/metabolism
MH  - Peptides
PMC - PMC10357481
OTO - NOTNLM
OT  - HLA ligandome
OT  - T-cell epitopes
OT  - breast cancer
OT  - extracellular vesicles
OT  - tumour associated antigen (TAA)
COIS- The authors declare no conflicts of interest.
EDAT- 2022/03/24 06:00
MHDA- 2022/05/24 06:00
PMCR- 2022/04/20
CRDT- 2022/03/23 05:49
PHST- 2021/12/16 00:00 [revised]
PHST- 2021/09/22 00:00 [received]
PHST- 2022/01/17 00:00 [accepted]
PHST- 2022/03/24 06:00 [pubmed]
PHST- 2022/05/24 06:00 [medline]
PHST- 2022/03/23 05:49 [entrez]
PHST- 2022/04/20 00:00 [pmc-release]
AID - IMM13471 [pii]
AID - 10.1111/imm.13471 [doi]
PST - ppublish
SO  - Immunology. 2022 Jun;166(2):249-264. doi: 10.1111/imm.13471. Epub 2022 Apr 20.