PMID- 35320932
OWN - NLM
STAT- MEDLINE
DCOM- 20220503
LR  - 20221207
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Epithelial Heat Shock Proteins Mediate the Protective Effects of 
      Limosilactobacillus reuteri in Dextran Sulfate Sodium-Induced Colitis.
PG  - 865982
LID - 10.3389/fimmu.2022.865982 [doi]
LID - 865982
AB  - Defects in gut barrier function are implicated in gastrointestinal (GI) disorders 
      like inflammatory bowel disease (IBD), as well as in systemic inflammation. With 
      the increasing incidence of IBD worldwide, more attention should be paid to 
      dietary interventions and therapeutics with the potential to boost the natural 
      defense mechanisms of gut epithelial cells. The current study aimed to 
      investigate the protective effects of Limosilactobacillus reuteri ATCC PTA 4659 
      in a colitis mouse model and delineate the mechanisms behind it. Wild-type mice 
      were allocated to the control group; or given 3% dextran sulfate sodium (DSS) in 
      drinking water for 7 days to induce colitis; or administered L. reuteri for 7 
      days as pretreatment; or for 14 days starting 7 days before subjecting to the 
      DSS. Peroral treatment with L. reuteri improved colitis severity clinically and 
      morphologically and reduced the colonic levels of Tumor necrosis factor-alpha (TNF-alpha) 
      (Tnf), Interleukin 1-beta (Il1beta), and nterferon-gamma (Ifng), the crucial 
      pro-inflammatory cytokines in colitis onset. It also prevented the 
      CD11b(+)Ly6G(+) neutrophil recruitment and the skewed immune responses in 
      mesenteric lymph nodes (MLNs) of CD11b(+)CD11c(+) dendritic cell (DC) expansion 
      and Foxp3(+)CD4(+) T-cell reduction. Using 16S rRNA gene amplicon sequencing and 
      RT-qPCR, we demonstrated a colitis-driven bacterial translocation to MLNs and gut 
      microbiota dysbiosis that were in part counterbalanced by L. reuteri treatment. 
      Moreover, the expression of barrier-preserving tight junction (TJ) proteins and 
      cytoprotective heat shock protein (HSP) 70 and HSP25 was reduced by colitis but 
      boosted by L. reuteri treatment. A shift in expression pattern was also observed 
      with HSP70 in response to the pretreatment and with HSP25 in response to L. 
      reuteri-DSS. In addition, the changes of HSPs were found to be correlated to 
      bacterial load and epithelial cell proliferation. In conclusion, our results 
      demonstrate that the human-derived L. reuteri strain 4659 confers protection in 
      experimental colitis in young mice, while intestinal HSPs may mediate the 
      probiotic effects by providing a supportive protein-protein network for the 
      epithelium in health and colitis.
CI  - Copyright (c) 2022 Liu, Gu, Zhu, Yuan, Zhu, Zhu, Yao, Hu, Zhang, Dicksved, Bao and 
      Cai.
FAU - Liu, Hao-Yu
AU  - Liu HY
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Gu, Fang
AU  - Gu F
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Zhu, Cuipeng
AU  - Zhu C
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Yuan, Long
AU  - Yuan L
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Zhu, Chuyang
AU  - Zhu C
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Zhu, Miaonan
AU  - Zhu M
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Yao, Jiacheng
AU  - Yao J
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Hu, Ping
AU  - Hu P
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Zhang, Yunzeng
AU  - Zhang Y
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China.
FAU - Dicksved, Johan
AU  - Dicksved J
AD  - Department of Animal Nutrition and Management, Swedish University of Agricultural 
      Sciences, Uppsala, Sweden.
FAU - Bao, Wenbin
AU  - Bao W
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
FAU - Cai, Demin
AU  - Cai D
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220307
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Tight Junction Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - *Colitis/pathology
MH  - Dextran Sulfate/toxicity
MH  - Heat-Shock Proteins
MH  - *Inflammatory Bowel Diseases
MH  - *Limosilactobacillus reuteri/physiology
MH  - Mice
MH  - RNA, Ribosomal, 16S/genetics
MH  - Tight Junction Proteins
MH  - Tumor Necrosis Factor-alpha
PMC - PMC8934773
OTO - NOTNLM
OT  - barrier function
OT  - gut microbiota
OT  - heat shock proteins
OT  - inflammatory bowel disease
OT  - probiotics
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/03/25 06:00
MHDA- 2022/05/04 06:00
PMCR- 2022/01/01
CRDT- 2022/03/24 05:04
PHST- 2022/01/30 00:00 [received]
PHST- 2022/02/11 00:00 [accepted]
PHST- 2022/03/24 05:04 [entrez]
PHST- 2022/03/25 06:00 [pubmed]
PHST- 2022/05/04 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.865982 [doi]
PST - epublish
SO  - Front Immunol. 2022 Mar 7;13:865982. doi: 10.3389/fimmu.2022.865982. eCollection 
      2022.