PMID- 35321565
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20220607
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 31
DP  - 2022 Jan-Dec
TI  - Induction of Human Umbilical Mesenchymal Stem Cell Differentiation Into Retinal 
      Pigment Epithelial Cells Using a Transwell-Based Co-culture System.
PG  - 9636897221085901
LID - 10.1177/09636897221085901 [doi]
LID - 09636897221085901
AB  - There is an increasing interest in generating retinal pigment epithelial (RPE) 
      cells from stem cells for treating degenerative eye diseases. However, whether 
      human umbilical cord mesenchymal stem cells (HUCMSCs) can differentiate into 
      RPE-like cells in a co-culture system has not been fully understood. In this 
      study, induction of HUCMSC differentiation into RPE-like cells was performed by 
      co-culturing HUCMSCs and a human RPE-like cell line (ARPE19) in a transwell 
      system and then analyzed for biomarkers using quantitative reverse transcription 
      polymerase chain reaction (RT-PCR) and immunofluorescence staining technique. 
      Moreover, the functional characterization of induced cells was carried out by 
      examining their phagocytic and neurotrophic factor-secreting activities. Our 
      results showed that mRNA expressions of RPE-specific markers-MITF, OTX2, RPE65, 
      PEDF, PME17, and CRALBP-and protein markers-RPE65, CRALBP, and ZO-1-were 
      significantly increased in HUCMSC-derived RPE-like cells. Functional 
      characteristic studies showed that these induced cells were capable of engulfing 
      photoreceptor outer segments and secreting brain-derived neurotrophic factor 
      (BDNF) and glial-derived neurotrophic factor (GDNF), which are typical functions 
      of RPE-like cells. Overall, the study findings indicate that the morphology and 
      proliferation of HUCMSCs can be maintained in a serum-free medium, and 
      differentiation into RPE-like cells can be induced by simply co-culturing HUCMSCs 
      with ARPE19 cells. Thus, the study provides fundamental information regarding the 
      clinical-scale generation of RPE-like cells from HUCMSCs.
FAU - Chang, Yu-Hsun
AU  - Chang YH
AD  - Department of Pediatrics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Foundation 
      and Tzu Chi University, Hualien.
FAU - Kumar, V Bharath
AU  - Kumar VB
AUID- ORCID: 0000-0002-4786-9913
AD  - Department of Medical Laboratory Science and Biotechnology, Asia University, 
      Taichung.
FAU - Wen, Yao-Tseng
AU  - Wen YT
AD  - Department of Ophthalmology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi 
      Foundation and Tzu Chi University, Hualien.
FAU - Huang, Chih-Yang
AU  - Huang CY
AUID- ORCID: 0000-0003-2347-0411
AD  - Department of Medical Laboratory Science and Biotechnology, Asia University, 
      Taichung.
AD  - Department of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Hualien.
AD  - Graduate Institute of Biomedical Sciences, China Medical University, Taichung.
AD  - Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi 
      University of Science and Technology, Hualien.
AD  - Department of Medical Research, China Medical University Hospital, China Medical 
      University, Taichung.
FAU - Tsai, Rong-Kung
AU  - Tsai RK
AD  - Department of Ophthalmology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi 
      Foundation and Tzu Chi University, Hualien.
FAU - Ding, Dah-Ching
AU  - Ding DC
AUID- ORCID: 0000-0001-5105-068X
AD  - Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu 
      Chi Foundation and Tzu Chi University, Hualien.
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Retinal Pigments)
SB  - IM
MH  - Coculture Techniques
MH  - Epithelial Cells
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - *Retinal Pigment Epithelium
MH  - Retinal Pigments/metabolism
PMC - PMC8961389
OTO - NOTNLM
OT  - differentiation
OT  - mesenchymal stem cells
OT  - retinal pigment epithelium
OT  - transwell
OT  - umbilical cord
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2022/03/25 06:00
MHDA- 2022/04/05 06:00
PMCR- 2022/03/24
CRDT- 2022/03/24 05:29
PHST- 2022/03/24 05:29 [entrez]
PHST- 2022/03/25 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2022/03/24 00:00 [pmc-release]
AID - 10.1177_09636897221085901 [pii]
AID - 10.1177/09636897221085901 [doi]
PST - ppublish
SO  - Cell Transplant. 2022 Jan-Dec;31:9636897221085901. doi: 
      10.1177/09636897221085901.