PMID- 35321703
OWN - NLM
STAT- MEDLINE
DCOM- 20220325
LR  - 20220329
IS  - 2662-7671 (Electronic)
IS  - 2662-7671 (Linking)
VI  - 22
IP  - 1
DP  - 2022 Mar 23
TI  - Polyphenols in Ilex latifolia Thunb. inhibit human lung cancer cell line A549 by 
      regulation of the PI3K-Akt signaling pathway.
PG  - 85
LID - 10.1186/s12906-022-03568-3 [doi]
LID - 85
AB  - BACKGROUND: The leaves of the plant Ilex latifolia Thunb. can be made into Kuding 
      tea, which is a drink rich in polyphenols. This study aimed to observe the effect 
      of Ilex latifolia Thunb. polyphenols (ILTPs) on human lung cancer cell line A549 
      (A549 cells) by regulating the phosphatidylinositol 3-kinase/protein kinase B 
      (PI3K/Akt) signaling pathway. METHODS: In vitro cultured cells were treated with 
      ILTPs; the proliferation of A549 cells and BEAS-2B human normal lung epithelial 
      cells (Beas-2B cells) was observed using the 
      3-(4,5-dimethylazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the 
      survival status of A549 cells was observed by fluorescence staining. The 
      expression of A549 cells was observed by quantitative polymerase chain reaction 
      (qPCR) assay and Western blot analysis, while the compound composition of ILTPs 
      was detected using high-performance liquid chromatography (HPLC). RESULTS: The 
      experimental results showed that the proliferation of Beas-2B cells was 
      unaffected by treatment with 0-500 mug/mL of ILTPs, whereas the decreased 
      proliferation of A549 cells was observed with the increasing concentrations of 
      ILTPs. Additionally, ILTPs elevated the levels of lactate dehydrogenase (LDH) and 
      reactive oxygen species (ROS) and promoted apoptosis in A549 cells. The results 
      of qPCR experiments showed that ILTPs upregulated caspase-9 mRNA expression and 
      downregulated phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), 
      mammalian target of rapamycin (mTOR), B-cell lymphoma-2 (Bcl-2), nuclear 
      factor-kappaB (NF-kappaB), vascular endothelial growth factor (VEGF), hypoxia-inducible 
      factor-1 alpha (HIF-1alpha), and cyclooxygenase-2 (COX-2) expression in A549 cells. 
      The Western blot analysis results also showed that ILTPs could reduce the protein 
      expression of PI3K and Akt. The HPLC results showed that the main compounds 
      present in the ILTPs were rutin, kaempferol, isochlorogenic acid A, 
      isochlorogenic acid B, and isochlorogenic acid C. CONCLUSIONS: Thus, this study 
      indicated that the polyphenols of I. latifolia act as a class of natural 
      functional food materials that potently suppress cancer by exerting their 
      inhibitory effects on A549 cell proliferation through five key polyphenolic 
      compounds.
CI  - (c) 2022. The Author(s).
FAU - Chen, Jing
AU  - Chen J
AD  - Chongqing Key Laboratory of Translational Research for Cancer Metastasis and 
      Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 
      400030, China.
FAU - Du, Yesheng
AU  - Du Y
AD  - Chongqing Key Laboratory of Translational Research for Cancer Metastasis and 
      Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 
      400030, China.
FAU - Long, Yanyan
AU  - Long Y
AD  - Chongqing Key Laboratory of Translational Research for Cancer Metastasis and 
      Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 
      400030, China.
FAU - Tao, Dan
AU  - Tao D
AD  - Chongqing Key Laboratory of Translational Research for Cancer Metastasis and 
      Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 
      400030, China.
FAU - Hu, Mengyu
AU  - Hu M
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of Education 
      (Chongqing University), Chongqing University Cancer Hospital, Chongqing, 400030, 
      China.
FAU - Jiang, Yong
AU  - Jiang Y
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of Education 
      (Chongqing University), Chongqing University Cancer Hospital, Chongqing, 400030, 
      China.
FAU - Wan, Yue
AU  - Wan Y
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of Education 
      (Chongqing University), Chongqing University Cancer Hospital, Chongqing, 400030, 
      China.
FAU - Yang, Dingyi
AU  - Yang D
AD  - Chongqing Key Laboratory of Translational Research for Cancer Metastasis and 
      Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 
      400030, China. cqres@foxmail.com.
LA  - eng
PT  - Journal Article
DEP - 20220323
PL  - England
TA  - BMC Complement Med Ther
JT  - BMC complementary medicine and therapies
JID - 101761232
RN  - 0 (Polyphenols)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - A549 Cells
MH  - Humans
MH  - *Ilex/metabolism
MH  - *Lung Neoplasms/genetics
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Polyphenols/pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Signal Transduction
MH  - Vascular Endothelial Growth Factor A
PMC - PMC8943935
OTO - NOTNLM
OT  - Ilex latifolia Thunb
OT  - Lung cancer
OT  - MTT
OT  - PI3K-Akt signaling pathway
OT  - Polyphenols
COIS- The authors declare that they have no competing interests.
EDAT- 2022/03/25 06:00
MHDA- 2022/03/26 06:00
PMCR- 2022/03/23
CRDT- 2022/03/24 05:30
PHST- 2021/10/31 00:00 [received]
PHST- 2022/03/11 00:00 [accepted]
PHST- 2022/03/24 05:30 [entrez]
PHST- 2022/03/25 06:00 [pubmed]
PHST- 2022/03/26 06:00 [medline]
PHST- 2022/03/23 00:00 [pmc-release]
AID - 10.1186/s12906-022-03568-3 [pii]
AID - 3568 [pii]
AID - 10.1186/s12906-022-03568-3 [doi]
PST - epublish
SO  - BMC Complement Med Ther. 2022 Mar 23;22(1):85. doi: 10.1186/s12906-022-03568-3.