PMID- 35322186
OWN - NLM
STAT- MEDLINE
DCOM- 20221223
LR  - 20230915
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 92
IP  - 6
DP  - 2022 Dec
TI  - Hematological changes in neonatal mice with phlebotomy-induced anemia.
PG  - 1575-1579
LID - 10.1038/s41390-022-02023-w [doi]
AB  - BACKGROUND: Anemia is a nearly universal diagnosis in preterm infants, caused by 
      phlebotomy, and exacerbated by the underlying erythropoietic immaturity. Newborn 
      infants are exposed to the unique stressor of fetal-to-neonatal transition, which 
      requires significant adaptation ex utero. Accordingly, the preterm infant's 
      response to anemia may alter the ability to confront underlying illness. This 
      study utilized our preclinical mouse model of phlebotomy-induced anemia (PIA) to 
      comprehensively investigate associated hematological changes. METHODS: C57BL/6 
      mice were subjected to timed phlebotomy between postnatal days 2--10 to induce 
      severe anemia. Complete blood counts were determined by the Sysmex XT-2000iV 
      analyzer. RESULTS: Anemic pups showed a gradual reduction of RBC and hemoglobin 
      (Hb) and increased reticulocyte (RET) counts and red cell distribution width 
      (RDW), however, with reduced RET-Hb from postnatal day (P) of 4 onwards. Elevated 
      levels of high fluorescent RET and immature reticulocyte fraction (IRF) were 
      noted in anemic mouse pups, but low and medium fluorescent RET were reduced. 
      Also, the reduction of mean corpuscular volume (MCV), mean corpuscular hemoglobin 
      (MCH), and mean corpuscular hemoglobin concentration (MCHC) were noted in anemic 
      pups. No changes were seen in lymphocytes, but monocytes and neutrophils were 
      significantly elevated from P4-P6. CONCLUSIONS: PIA in mouse pups is associated 
      with hematological changes that may be exacerbating factors in neonatal diseases. 
      IMPACT: Anemia is common and often severe in premature infants. Investigation of 
      hematological parameters in settings of preclinical anemia may be an index of 
      therapeutic strategies. Preclinical model evaluating the effects of neonatal 
      anemia on the remainder of complete blood count. Detailed time kinetic 
      phlebotomy-induced anemic mice enable us to study the impact on developmental 
      delays in erythropoiesis and possible strategic intervention. Hematological 
      effects of severe anemia in mice might provide insight on how best to investigate 
      anemia in preterm infants.
CI  - (c) 2022. The Author(s), under exclusive licence to the International Pediatric 
      Research Foundation, Inc.
FAU - Chung, Yerin
AU  - Chung Y
AD  - Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, 
      MD, 21287, USA.
FAU - Desiraju, Suneetha
AU  - Desiraju S
AD  - Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, 
      MD, 21287, USA.
FAU - Namachivayam, Kopperuncholan
AU  - Namachivayam K
AD  - Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, 
      MD, 21287, USA.
FAU - Guzman, Pierre
AU  - Guzman P
AD  - Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, 
      MD, 21287, USA.
FAU - He, Ling
AU  - He L
AD  - Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, 
      MD, 21287, USA.
AD  - Department of Pharmacology & Molecular Sciences, Johns Hopkins University School 
      of Medicine, Baltimore, MD, 21287, USA.
FAU - MohanKumar, Krishnan
AU  - MohanKumar K
AD  - Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, 
      MD, 21287, USA. mohank@jhmi.edu.
LA  - eng
GR  - R01 HL133022/HL/NHLBI NIH HHS/United States
GR  - R01 HL124078/HL/NHLBI NIH HHS/United States
GR  - R01 HL163043/HL/NHLBI NIH HHS/United States
GR  - R21 HD105880/HD/NICHD NIH HHS/United States
GR  - R01 DK120309/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220323
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
SB  - IM
MH  - Infant, Newborn
MH  - Humans
MH  - Animals
MH  - Mice
MH  - Animals, Newborn
MH  - *Phlebotomy/adverse effects
MH  - Infant, Premature
MH  - Mice, Inbred C57BL
MH  - *Anemia/complications
PMC - PMC9500113
MID - NIHMS1793401
COIS- COMPETING INTERESTS The authors declare no competing interests.
EDAT- 2022/03/25 06:00
MHDA- 2022/12/24 06:00
PMCR- 2022/12/24
CRDT- 2022/03/24 05:38
PHST- 2021/10/06 00:00 [received]
PHST- 2022/03/06 00:00 [accepted]
PHST- 2021/11/18 00:00 [revised]
PHST- 2022/03/25 06:00 [pubmed]
PHST- 2022/12/24 06:00 [medline]
PHST- 2022/03/24 05:38 [entrez]
PHST- 2022/12/24 00:00 [pmc-release]
AID - 10.1038/s41390-022-02023-w [pii]
AID - 10.1038/s41390-022-02023-w [doi]
PST - ppublish
SO  - Pediatr Res. 2022 Dec;92(6):1575-1579. doi: 10.1038/s41390-022-02023-w. Epub 2022 
      Mar 23.