PMID- 35322864
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220531
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 25
IP  - 5
DP  - 2022 May
TI  - lncRNA LINC00284 promotes nucleus pulposus cell proliferation and ECM synthesis 
      via regulation of the miR‑205‑3p/Wnt/beta‑catenin axis.
LID - 179 [pii]
LID - 10.3892/mmr.2022.12695 [doi]
AB  - Intervertebral disc degeneration (IDD) is a leading cause of degenerative spinal 
      disease. Long non‑coding RNA (lncRNA) LINC00284 is overexpressed in multiple 
      types of cancer and promotes cancer cell proliferation and inhibits apoptosis; 
      however, its role in human IDD and nucleus pulposus (NP) remain unclear. In the 
      present study, intervertebral disc (IVD) tissues were collected from IDD patients 
      for detection of LINC00284 expression using reverse transcription‑quantitative 
      PCR, the binding effect between miR‑205‑3p and LINC00284 was validated by 
      dual‑luciferase reporter assay. miR‑205‑3p and small interfering RNA (siRNA) was 
      used for LINC00240 knockdown to investigate the proliferation, apoptosis of cells 
      in the NP cells measured by Cell Counting Kit (CCK)‑8 assay and 
      Annexin V‑FITC/Propidium Iodide (PI) staining with flow cytometry receptivity. 
      IDD animal models were constructed for in vivo study of the role LINC00284 in IDD 
      improvement. The results showed that LINC00284 expression was upregulated in IDD 
      tissue and IL‑1beta‑induced NP cells. LINC00284 knockdown resulted in an increase in 
      IL‑1beta‑induced NP cell proliferation, a decrease in apoptosis and matrix 
      metalloproteinase‑3 expression and an increase in expression of extracellular 
      matrix (ECM) markers aggrecan and collagen II. In vivo experiments and 
      histomorphometric analysis confirmed the protective effect of LINC00284 knockdown 
      in IDD. LINC00284 was also shown to be a target of microRNA (miR)‑205‑3p, and 
      there was a negative correlation between LINC00284 and miR‑205‑3p levels in IDD 
      tissue. Additionally, LINC00284 knockdown or miR‑205‑3p upregulation resulted in 
      inhibition of Wnt/beta‑catenin signaling and subsequent degradation of the ECM. The 
      present study demonstrated that LINC00284 activated the Wnt/beta‑catenin signaling 
      via sponging miR‑205‑3p, resulting in inhibition of NP cell proliferation and ECM 
      synthesis. These results suggested that targeting LINC00284 to rescue miR‑205‑3p 
      expression may be a potential method for IDD management.
FAU - Zhu, Min
AU  - Zhu M
AD  - Department of Spine Surgery, The Second Affiliated Hospital of Soochow 
      University, Suzhou, Jiangsu 215004, P.R. China.
FAU - Yan, Xiaoling
AU  - Yan X
AD  - Chemotherapy Department, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, P.R. China.
FAU - Zhao, Yin
AU  - Zhao Y
AD  - Department of Spine Surgery, Shanghai Changzheng Hospital, The Second Military 
      Medical University, Shanghai 200003, P.R. China.
FAU - Xue, Huawei
AU  - Xue H
AD  - Department of Spine Surgery, Nantong Third People's Hospital, Nantong, Jiangsu 
      226006, P.R. China.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Department of Spine Surgery, Nantong Third People's Hospital, Nantong, Jiangsu 
      226006, P.R. China.
FAU - Wu, Bo
AU  - Wu B
AD  - Department of Spine Surgery, Nantong Third People's Hospital, Nantong, Jiangsu 
      226006, P.R. China.
FAU - Li, Xiangyang
AU  - Li X
AD  - Department of Spine Surgery, Nantong Third People's Hospital, Nantong, Jiangsu 
      226006, P.R. China.
FAU - Shen, Yixin
AU  - Shen Y
AD  - Department of Spine Surgery, The Second Affiliated Hospital of Soochow 
      University, Suzhou, Jiangsu 215004, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20220324
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (MIRN205 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Cell Proliferation/genetics
MH  - Cells, Cultured
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - *Intervertebral Disc Degeneration/genetics/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *Nucleus Pulposus/cytology
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Wnt Signaling Pathway
MH  - beta Catenin/metabolism
PMC - PMC8972274
OTO - NOTNLM
OT  - Wnt/beta‑catenin signaling
OT  - intervertebral disc degeneration
OT  - long non‑coding RNA LINC00284
OT  - microRNA‑205‑3p
COIS- The authors declare that they have no competing interests.
EDAT- 2022/03/25 06:00
MHDA- 2022/04/08 06:00
PMCR- 2022/03/22
CRDT- 2022/03/24 08:40
PHST- 2021/08/12 00:00 [received]
PHST- 2022/02/23 00:00 [accepted]
PHST- 2022/03/24 08:40 [entrez]
PHST- 2022/03/25 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/03/22 00:00 [pmc-release]
AID - 179 [pii]
AID - MMR-25-05-12695 [pii]
AID - 10.3892/mmr.2022.12695 [doi]
PST - ppublish
SO  - Mol Med Rep. 2022 May;25(5):179. doi: 10.3892/mmr.2022.12695. Epub 2022 Mar 24.