PMID- 35325716
OWN - NLM
STAT- MEDLINE
DCOM- 20220428
LR  - 20230314
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 207
DP  - 2022 Jul 1
TI  - Longitudinal morphological and functional characterization of human heart 
      organoids using optical coherence tomography.
PG  - 114136
LID - S0956-5663(22)00176-2 [pii]
LID - 10.1016/j.bios.2022.114136 [doi]
AB  - Organoids play an increasingly important role as in vitro models for studying 
      organ development, disease mechanisms, and drug discovery. Organoids are 
      self-organizing, organ-like three-dimensional (3D) cell cultures developing 
      organ-specific cell types and functions. Recently, three groups independently 
      developed self-assembling human heart organoids (hHOs) from human pluripotent 
      stem cells (hPSCs). In this study, we utilized a customized spectral-domain 
      optical coherence tomography (SD-OCT) system to characterize the growth of hHOs. 
      Development of chamber structures and beating patterns of the hHOs were observed 
      via OCT and calcium imaging. We demonstrated the capability of OCT to produce 3D 
      images in a fast, label-free, and non-destructive manner. The hHOs formed 
      cavities of various sizes, and complex interconnections were observed as early as 
      on day 4 of differentiation. The hHOs models and the OCT imaging system showed 
      promising insights as an in vitro platform for investigating heart development 
      and disease mechanisms.
CI  - Copyright (c) 2022 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Ming, Yixuan
AU  - Ming Y
AD  - Department of Biomedical Engineering, Washington University in Saint Louis, USA.
FAU - Hao, Senyue
AU  - Hao S
AD  - Department of Electrical & Systems Engineering, Washington University in Saint 
      Louis, USA.
FAU - Wang, Fei
AU  - Wang F
AD  - Department of Biomedical Engineering, Washington University in Saint Louis, USA.
FAU - Lewis-Israeli, Yonatan R
AU  - Lewis-Israeli YR
AD  - Institute for Quantitative Health Science and Engineering, Division of 
      Developmental and Stem Cell Biology, Michigan State University, USA; Department 
      of Biomedical Engineering, College of Engineering, Michigan State University, 
      USA.
FAU - Volmert, Brett D
AU  - Volmert BD
AD  - Institute for Quantitative Health Science and Engineering, Division of 
      Developmental and Stem Cell Biology, Michigan State University, USA; Department 
      of Biomedical Engineering, College of Engineering, Michigan State University, 
      USA.
FAU - Xu, Zhiyao
AU  - Xu Z
AD  - Department of Biomedical Engineering, Washington University in Saint Louis, USA.
FAU - Goestenkors, Anna
AU  - Goestenkors A
AD  - Department of Biomedical Engineering, Washington University in Saint Louis, USA.
FAU - Aguirre, Aitor
AU  - Aguirre A
AD  - Institute for Quantitative Health Science and Engineering, Division of 
      Developmental and Stem Cell Biology, Michigan State University, USA; Department 
      of Biomedical Engineering, College of Engineering, Michigan State University, 
      USA.
FAU - Zhou, Chao
AU  - Zhou C
AD  - Department of Biomedical Engineering, Washington University in Saint Louis, USA. 
      Electronic address: chaozhou@wustl.edu.
LA  - eng
GR  - K01 HL135464/HL/NHLBI NIH HHS/United States
GR  - R01 EB025209/EB/NIBIB NIH HHS/United States
GR  - R01 HL151505/HL/NHLBI NIH HHS/United States
GR  - R01 HL156265/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20220309
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - *Biosensing Techniques
MH  - Cell Differentiation
MH  - Humans
MH  - Organoids
MH  - *Pluripotent Stem Cells
MH  - Tomography, Optical Coherence/methods
PMC - PMC9713770
MID - NIHMS1850698
OTO - NOTNLM
OT  - Chambers
OT  - Heart organoids
OT  - Optical coherence tomography
OT  - hPSC
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/03/25 06:00
MHDA- 2022/04/29 06:00
PMCR- 2022/12/01
CRDT- 2022/03/24 20:12
PHST- 2022/01/08 00:00 [received]
PHST- 2022/02/17 00:00 [revised]
PHST- 2022/02/24 00:00 [accepted]
PHST- 2022/03/25 06:00 [pubmed]
PHST- 2022/04/29 06:00 [medline]
PHST- 2022/03/24 20:12 [entrez]
PHST- 2022/12/01 00:00 [pmc-release]
AID - S0956-5663(22)00176-2 [pii]
AID - 10.1016/j.bios.2022.114136 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2022 Jul 1;207:114136. doi: 10.1016/j.bios.2022.114136. Epub 
      2022 Mar 9.