PMID- 35326196
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220329
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 11
IP  - 3
DP  - 2022 Mar 14
TI  - ALA_PDT Promotes Ferroptosis-Like Death of Mycobacterium abscessus and Antibiotic 
      Sterilization via Oxidative Stress.
LID - 10.3390/antiox11030546 [doi]
LID - 546
AB  - Mycobacterium abscessus is one of the common clinical non-tuberculous 
      mycobacteria (NTM) that can cause severe skin infection. 5-Aminolevulinic acid 
      photodynamic therapy (ALA_PDT) is an emerging effective antimicrobial treatment. 
      To explore whether ALA_PDT can be used to treat M. abscessus infections, we 
      conducted a series of experiments in vitro. We found that ALA_PDT can kill M. 
      abscesses. Mechanistically, we found that ALA_PDT promoted ferroptosis-like death 
      of M. abscesses, and the ROS scavenger N-Acetyl-L-cysteine (NAC) and ferroptosis 
      inhibitor Ferrostatin-1 (Fer-1) can mitigate the ALA_PDT-mediated sterilization. 
      Furthermore, ALA_PDT significantly up-regulated the transcription of heme 
      oxygenase MAB_4773, increased the intracellular Fe(2+) concentration and altered 
      the transcription of M. abscessus iron metabolism genes. ALA_PDT disrupted the 
      integrity of the cell membrane and enhanced the permeability of the cell 
      membrane, as evidenced by the boosted sterilization effect of antibiotics. In 
      summary, ALA_PDT can kill M. abscesses via promoting the ferroptosis-like death 
      and antibiotic sterilization through oxidative stress by changing iron 
      metabolism. The study provided new mechanistic insights into the clinical 
      efficacy of ALA_PDT against M. abscessus.
FAU - Wang, Xiaoyu
AU  - Wang X
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education, 
      College of Bioengineering, Chongqing University, Chongqing 400044, China.
AD  - Department of Dermatology, Daping Hospital, The Army Medical University, 
      Chongqing 400044, China.
AD  - Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of 
      Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of 
      Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, 
      College of Life and Sciences, Southwest University, Chongqing 400700, China.
FAU - Wan, Meiyin
AU  - Wan M
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education, 
      College of Bioengineering, Chongqing University, Chongqing 400044, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of 
      Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of 
      Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, 
      College of Life and Sciences, Southwest University, Chongqing 400700, China.
FAU - Dai, Yongdong
AU  - Dai Y
AD  - Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of 
      Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of 
      Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, 
      College of Life and Sciences, Southwest University, Chongqing 400700, China.
FAU - Hai, Yang
AU  - Hai Y
AD  - Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of 
      Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of 
      Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, 
      College of Life and Sciences, Southwest University, Chongqing 400700, China.
FAU - Yue, Chenda
AU  - Yue C
AD  - Department of Dermatology, Daping Hospital, The Army Medical University, 
      Chongqing 400044, China.
FAU - Xu, Junqi
AU  - Xu J
AD  - Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of 
      Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of 
      Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, 
      College of Life and Sciences, Southwest University, Chongqing 400700, China.
FAU - Ding, Yadan
AU  - Ding Y
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education, 
      College of Bioengineering, Chongqing University, Chongqing 400044, China.
FAU - Wang, Mei
AU  - Wang M
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education, 
      College of Bioengineering, Chongqing University, Chongqing 400044, China.
FAU - Xie, Jianping
AU  - Xie J
AD  - Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of 
      Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of 
      Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, 
      College of Life and Sciences, Southwest University, Chongqing 400700, China.
FAU - Lei, Xia
AU  - Lei X
AD  - Department of Dermatology, Daping Hospital, The Army Medical University, 
      Chongqing 400044, China.
FAU - Zhong, Julia-Li
AU  - Zhong JL
AD  - Key Laboratory of Biorheological Science and Technology, Ministry of Education, 
      College of Bioengineering, Chongqing University, Chongqing 400044, China.
LA  - eng
GR  - 81773348/National Natural Science Foundation of China/
GR  - 82072246/National Natural Science Foundation of China/
GR  - 81871182/National Natural Science Foundation of China/
GR  - 7820100597/2020 Chongqing Post-Doctoral Research Project Special Funding/
GR  - ZK-2021-565/The Science and Technology Plan Project of Guizhou Province/
GR  - 2018-5772-072/The Initial Research Funds for Ph.D in Zunyi Medical University/
GR  - 2018-5772-066/2020 City-School Joint Project/
PT  - Journal Article
DEP - 20220314
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC8945036
OTO - NOTNLM
OT  - ALA_PDT
OT  - Mycobacterium abscessus
OT  - antibiotic resistance
OT  - ferroptosis
OT  - heme oxygenase MAB_4773
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/03/26 06:00
MHDA- 2022/03/26 06:01
PMCR- 2022/03/14
CRDT- 2022/03/25 01:00
PHST- 2022/01/24 00:00 [received]
PHST- 2022/03/06 00:00 [revised]
PHST- 2022/03/11 00:00 [accepted]
PHST- 2022/03/25 01:00 [entrez]
PHST- 2022/03/26 06:00 [pubmed]
PHST- 2022/03/26 06:01 [medline]
PHST- 2022/03/14 00:00 [pmc-release]
AID - antiox11030546 [pii]
AID - antioxidants-11-00546 [pii]
AID - 10.3390/antiox11030546 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2022 Mar 14;11(3):546. doi: 10.3390/antiox11030546.