PMID- 35327493
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220329
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 10
IP  - 3
DP  - 2022 Mar 17
TI  - The Potential Role of Peripheral Oxidative Stress on the Neurovascular Unit in 
      Amyotrophic Lateral Sclerosis Pathogenesis: A Preliminary Report from Human and 
      In Vitro Evaluations.
LID - 10.3390/biomedicines10030691 [doi]
LID - 691
AB  - Oxidative stress, the alteration of mitochondrial function, and changes in the 
      neurovascular unit (NVU) could play a role in Amyotrophic Lateral Sclerosis (ALS) 
      pathogenesis. Our aim was to analyze the plasma redox system and nitric oxide 
      (NO) in 25 ALS new-diagnosed patients and five healthy controls and the effects 
      of plasma on the peroxidation/mitochondrial function in human umbilical 
      cord-derived endothelial vascular cells (HUVEC) and astrocytes. In plasma, 
      thiobarbituric acid reactive substances (TBARS), glutathione (GSH), and nitric 
      oxide (NO) were analyzed by using specific assays. In HUVEC/astrocytes, the 
      effects of plasma on the release of mitochondrial reactive oxygen species 
      (mitoROS) and NO, viability, and mitochondrial membrane potential were 
      investigated. In the plasma of ALS patients, an increase in TBARS and a reduction 
      in GSH and NO were found. In HUVEC/astrocytes treated with a plasma of ALS 
      patients, mitoROS increased, whereas cell viability and mitochondrial membrane 
      potential decreased. Our results show that oxidative stress and NVU play a 
      central role in ALS and suggest that unknown plasma factors could be involved in 
      the disease pathogenesis. Quantifiable changes in ALS plasma related to redox 
      state alterations can possibly be used for early diagnosis.
FAU - Grossini, Elena
AU  - Grossini E
AUID- ORCID: 0000-0002-3012-259X
AD  - Laboratory of Physiology, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
FAU - Garhwal, Divya
AU  - Garhwal D
AD  - Laboratory of Physiology, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
FAU - Venkatesan, Sakthipriyan
AU  - Venkatesan S
AUID- ORCID: 0000-0002-7517-2574
AD  - Laboratory of Physiology, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
FAU - Ferrante, Daniela
AU  - Ferrante D
AD  - Statistic Unit, Department of Translational Medicine, University East Piedmont, 
      28100 Novara, Italy.
FAU - Mele, Angelica
AU  - Mele A
AD  - ALS Center, Neurology Unit, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
FAU - Saraceno, Massimo
AU  - Saraceno M
AD  - ALS Center, Neurology Unit, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
FAU - Scognamiglio, Ada
AU  - Scognamiglio A
AD  - ALS Center, Neurology Unit, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
FAU - Mandrioli, Jessica
AU  - Mandrioli J
AUID- ORCID: 0000-0002-9244-9782
AD  - Department of Biomedical, Metabolic and Neural Sciences, University of Modena and 
      Reggio Emilia, 41125 Modena, Italy.
AD  - Neurology Unit, Azienda Ospedaliero Universitaria di Modena, 41126 Modena, Italy.
FAU - Amedei, Amedeo
AU  - Amedei A
AUID- ORCID: 0000-0002-6797-9343
AD  - Department of Experimental and Clinical Medicine, University of Florence, 50134 
      Florence, Italy.
FAU - De Marchi, Fabiola
AU  - De Marchi F
AUID- ORCID: 0000-0003-0197-1880
AD  - ALS Center, Neurology Unit, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
FAU - Mazzini, Letizia
AU  - Mazzini L
AD  - ALS Center, Neurology Unit, Department of Translational Medicine, University East 
      Piedmont, 28100 Novara, Italy.
LA  - eng
PT  - Journal Article
DEP - 20220317
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC8945260
OTO - NOTNLM
OT  - astrocytes
OT  - endothelial cells
OT  - mitochondria
OT  - nitric oxide
OT  - oxidative stress
COIS- The authors declare that they have no competing interests.
EDAT- 2022/03/26 06:00
MHDA- 2022/03/26 06:01
PMCR- 2022/03/17
CRDT- 2022/03/25 01:04
PHST- 2022/01/24 00:00 [received]
PHST- 2022/03/08 00:00 [revised]
PHST- 2022/03/14 00:00 [accepted]
PHST- 2022/03/25 01:04 [entrez]
PHST- 2022/03/26 06:00 [pubmed]
PHST- 2022/03/26 06:01 [medline]
PHST- 2022/03/17 00:00 [pmc-release]
AID - biomedicines10030691 [pii]
AID - biomedicines-10-00691 [pii]
AID - 10.3390/biomedicines10030691 [doi]
PST - epublish
SO  - Biomedicines. 2022 Mar 17;10(3):691. doi: 10.3390/biomedicines10030691.