PMID- 35327987
OWN - NLM
STAT- MEDLINE
DCOM- 20220427
LR  - 20220531
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 13
IP  - 3
DP  - 2022 Feb 26
TI  - Long Intergenic Non-Protein Coding RNA 02381 Promotes the Proliferation and 
      Invasion of Ovarian Endometrial Stromal Cells through the miR-27b-3p/CTNNB1 Axis.
LID - 10.3390/genes13030433 [doi]
LID - 433
AB  - PURPOSE: Catenin Beta 1 (CTNNB1) is a key regulator of cell proliferation and 
      invasion in endometriosis; however, its upstream factor is not clear. Long 
      noncoding RNAs may participate in endometriosis. The aim of this study was to 
      investigate the mechanism of interaction between LINC02381 and CTNNB1 in 
      endometriosis. METHOD: Screening and validation of RNAs were completed by whole 
      transcriptional sequencing and qRT-PCR. The subcellular localization of LINC02381 
      was determined by RNA in situ hybridization and nucleo-cytoplasmic separation. 
      Plasmids were transfected for functional experiments. Luciferase assay was used 
      to verify the binding relationship. RESULTS: The expression of LINC02381 and 
      CTNNB1 was significantly increased in ovarian ectopic endometrial tissues (OSAs) 
      and ectopic endometrial stromal cells (ESCs). When LINC02381 was downregulated in 
      ESCs, the expression of CTNNB1, metallopeptidase 9 (MMP9) and cyclinD1, as well 
      as ESCs invasion and proliferation, decreased. LINC02381 was mainly present in 
      the cytoplasm of ESCs, indicating that it may act as a competitive endogenous 
      RNA. Bioinformatic analysis revealed that microRNA-27b-3p (miR-27b-3p) is a 
      downstream target of LINC02381. miR-27b-3p decreased in OSAs and ESCs. Moreover, 
      when miR-27b-3p was upregulated in ESCs, the expression of CTNNB1, MMP9 and 
      cyclinD1, as well as the invasion and proliferation ability of ESCs, were 
      reduced. Additionally, rescue experiments demonstrated that the expression of 
      CTNNB1, MMP9 and cyclinD1, as well as the invasion and proliferation ability, 
      were significantly increased in the group transfected with both sh-LINC02381 and 
      a miR-27b-3p inhibitor. CONCLUSION: LINC02381 upregulated CTNNB1 by adsorbing 
      miR-27b-3p, causing increased proliferation and invasion of ESCs.
FAU - Wang, Xiaoqing
AU  - Wang X
AD  - Department of Obstetrics and Gynecology, Peking University First Hospital, 
      Beijing 100034, China.
FAU - Wu, Peili
AU  - Wu P
AD  - Department of Obstetrics and Gynecology, Peking University First Hospital, 
      Beijing 100034, China.
FAU - Zeng, Cheng
AU  - Zeng C
AD  - Department of Obstetrics and Gynecology, Peking University First Hospital, 
      Beijing 100034, China.
FAU - Zhu, Jingwen
AU  - Zhu J
AD  - Department of Obstetrics and Gynecology, Peking University First Hospital, 
      Beijing 100034, China.
FAU - Zhou, Yingfang
AU  - Zhou Y
AD  - Department of Obstetrics and Gynecology, Peking University First Hospital, 
      Beijing 100034, China.
FAU - Lu, Ye
AU  - Lu Y
AD  - Department of Obstetrics and Gynecology, Peking University First Hospital, 
      Beijing 100034, China.
FAU - Xue, Qing
AU  - Xue Q
AD  - Department of Obstetrics and Gynecology, Peking University First Hospital, 
      Beijing 100034, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220226
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (beta Catenin)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Endometriosis/genetics
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 9
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Sleep Apnea, Obstructive
MH  - Stromal Cells/metabolism
MH  - beta Catenin/genetics
PMC - PMC8955621
OTO - NOTNLM
OT  - CTNNB1
OT  - LINC02381
OT  - endometriosis
OT  - invasion
OT  - miR-27b-3p
OT  - proliferation
COIS- There are no conflicts of interest to declare.
EDAT- 2022/03/26 06:00
MHDA- 2022/04/28 06:00
PMCR- 2022/02/26
CRDT- 2022/03/25 01:05
PHST- 2022/01/16 00:00 [received]
PHST- 2022/02/20 00:00 [revised]
PHST- 2022/02/24 00:00 [accepted]
PHST- 2022/03/25 01:05 [entrez]
PHST- 2022/03/26 06:00 [pubmed]
PHST- 2022/04/28 06:00 [medline]
PHST- 2022/02/26 00:00 [pmc-release]
AID - genes13030433 [pii]
AID - genes-13-00433 [pii]
AID - 10.3390/genes13030433 [doi]
PST - epublish
SO  - Genes (Basel). 2022 Feb 26;13(3):433. doi: 10.3390/genes13030433.