PMID- 35330015
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220330
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 11
IP  - 6
DP  - 2022 Mar 18
TI  - Epitope Mapping of Pathogenic Autoantigens on Sjogren's Syndrome-Susceptible 
      Human Leukocyte Antigens Using In Silico Techniques.
LID - 10.3390/jcm11061690 [doi]
LID - 1690
AB  - Sjogren's syndrome (SjS) is characterized by lymphocytic infiltration and the 
      dysfunction of the salivary and lacrimal glands. The autoimmune response is 
      driven by the effector T cells and their cytokines. The activation of the 
      effector helper T cells is mediated by autoantigen presentation by human 
      leukocyte antigen (HLA) class II molecules of antigen-presenting cells. Studies 
      using familial aggregation, animal models, and genome-wide association 
      demonstrate a significant genetic correlation between specific risk HLAs and SjS. 
      One of the key HLA alleles is HLA-DRB1*0301; it is one of the most influential 
      associations with primary SjS, having the highest odds ratio and occurrence 
      across different ethnic groups. The specific autoantigens attributed to SjS 
      remain elusive, especially the specific antigenic epitopes presented by 
      HLA-DRB1*0301. This study applied a high throughput in silico mapping technique 
      to identify antigenic epitopes of known SjS autoantigens presented by high-risk 
      HLAs. Furthermore, we identified specific binding HLA-DRB1*0301 epitopes using 
      structural modeling tools such as Immune Epitope Database and Analysis Resource 
      IEDB, AutoDock Vina, and COOT. By deciphering the critical epitopes of 
      autoantigens presented by HLA-DRB1*0301, we gain a better understanding of the 
      origin of the antigens, determine the T cell receptor function, learn the 
      mechanism of disease progression, and develop therapeutic applications.
FAU - Gupta, Shivai
AU  - Gupta S
AD  - Department of Infectious Diseases and Immunology, College of Veterinary Medicine, 
      University of Florida, Gainesville, FL 32611, USA.
FAU - Li, Danmeng
AU  - Li D
AUID- ORCID: 0000-0002-6905-554X
AD  - Department of Pathology, Immunology & Laboratory Medicine, College of Medicine, 
      University of Florida, Gainesville, FL 32610, USA.
FAU - Ostrov, David A
AU  - Ostrov DA
AUID- ORCID: 0000-0002-4696-875X
AD  - Department of Pathology, Immunology & Laboratory Medicine, College of Medicine, 
      University of Florida, Gainesville, FL 32610, USA.
FAU - Nguyen, Cuong Q
AU  - Nguyen CQ
AUID- ORCID: 0000-0001-5633-4075
AD  - Department of Infectious Diseases and Immunology, College of Veterinary Medicine, 
      University of Florida, Gainesville, FL 32611, USA.
AD  - Department of Oral Biology, College of Dentistry, University of Florida, 
      Gainesville, FL 32610, USA.
AD  - Center of Orphaned Autoimmune Diseases, University of Florida, Gainesville, FL 
      32611, USA.
LA  - eng
GR  - R01 DE028544/DE/NIDCR NIH HHS/United States
PT  - Journal Article
DEP - 20220318
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC8953074
OTO - NOTNLM
OT  - T cells
OT  - autoantigens
OT  - human leukocyte antigen (HLA)
OT  - major histocompatibility complex (MHC)
COIS- The authors declare no conflict of interest.
EDAT- 2022/03/26 06:00
MHDA- 2022/03/26 06:01
PMCR- 2022/03/18
CRDT- 2022/03/25 01:12
PHST- 2022/02/02 00:00 [received]
PHST- 2022/03/03 00:00 [revised]
PHST- 2022/03/08 00:00 [accepted]
PHST- 2022/03/25 01:12 [entrez]
PHST- 2022/03/26 06:00 [pubmed]
PHST- 2022/03/26 06:01 [medline]
PHST- 2022/03/18 00:00 [pmc-release]
AID - jcm11061690 [pii]
AID - jcm-11-01690 [pii]
AID - 10.3390/jcm11061690 [doi]
PST - epublish
SO  - J Clin Med. 2022 Mar 18;11(6):1690. doi: 10.3390/jcm11061690.