PMID- 35330291
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220328
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 8
IP  - 3
DP  - 2022 Mar 11
TI  - Identification of Mucormycosis by Fluorescence In Situ Hybridization Targeting 
      Ribosomal RNA in Tissue Samples.
LID - 10.3390/jof8030289 [doi]
LID - 289
AB  - Mucormycosis is an invasive fungal infection associated with high mortality, 
      partly due to delayed diagnosis and inadequate empiric therapy. As fungal 
      cultures often fail to grow Mucorales, identification of respective hyphae in 
      tissue is frequently needed for diagnosis but may be challenging. We studied 
      fluorescence in situ hybridization (FISH) targeting specific regions of the 
      fungal ribosomal RNA (rRNA) of Mucorales to improve diagnosis of mucormycosis 
      from tissue samples. We generated a probe combination specifically targeting 
      Mucorales. Probe specificity was verified in silico and using cultivated fungi. 
      Mucorales hyphae in tissue of a mouse model demonstrated a bright cytoplasmatic 
      hybridization signal. In tissue samples of patients with mucormycosis, a positive 
      signal was seen in 7 of 12 (58.3%) samples. However, autofluorescence in 3 of 7 
      (42.9%) samples impaired the diagnostic yield. Subsequent experiments suggested 
      that availability of nutrients and antifungal therapy may impact on the FISH 
      signal obtained with Mucorales hyphae. Diagnosis of mucormycosis from tissue 
      might be improved by rRNA FISH in a limited number of cases only. FISH signals 
      may reflect different physiological states of fungi in tissue. Further studies 
      are needed to define the value of FISH to diagnose mucormycosis from other 
      clinical samples.
FAU - Dalimot, Jill Jasmine
AU  - Dalimot JJ
AUID- ORCID: 0000-0002-1306-093X
AD  - Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, 
      Robert Koch Institute (RKI), 13353 Berlin, Germany.
AD  - Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, 
      London NW1 1AT, UK.
FAU - Smith, Ilka Mc Cormick
AU  - Smith IMC
AD  - Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, 
      Robert Koch Institute (RKI), 13353 Berlin, Germany.
FAU - Gerkrath, Jasmin
AU  - Gerkrath J
AD  - Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, 
      Robert Koch Institute (RKI), 13353 Berlin, Germany.
FAU - Hartmann, Sylvia
AU  - Hartmann S
AUID- ORCID: 0000-0003-3424-1091
AD  - Senckenberg Institute for Pathology, Johann Wolfgang Goethe University Frankfurt, 
      60323 Frankfurt am Main, Germany.
FAU - Cornely, Oliver A
AU  - Cornely OA
AUID- ORCID: 0000-0001-9599-3137
AD  - Excellence Center for Medical Mycology (ECMM), Department I of Internal Medicine, 
      Faculty of Medicine and University Hospital Cologne, University of Cologne, 50923 
      Cologne, Germany.
AD  - Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated 
      Diseases (CECAD), Chair Translational Research, Faculty of Medicine and 
      University Hospital Cologne, University of Cologne, 50923 Cologne, Germany.
AD  - Clinical Trials Centre Cologne (ZKS Koln), Faculty of Medicine and University 
      Hospital Cologne, University of Cologne, 50923 Cologne, Germany.
AD  - German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, 50923 
      Cologne, Germany.
FAU - Lee, Soo Chan
AU  - Lee SC
AUID- ORCID: 0000-0002-7792-5947
AD  - Department of Molecular Genetics and Microbiology, Duke University Medical 
      Center, Durham, NC 27710, USA.
AD  - South Texas Center for Emerging Infectious Diseases (STCEID), Department of 
      Molecular Microbiology and Immunology, The University of Texas, San Antonio, TX 
      78249, USA.
FAU - Heitman, Joseph
AU  - Heitman J
AD  - Department of Molecular Genetics and Microbiology, Duke University Medical 
      Center, Durham, NC 27710, USA.
FAU - Rickerts, Volker
AU  - Rickerts V
AUID- ORCID: 0000-0002-6154-4920
AD  - Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, 
      Robert Koch Institute (RKI), 13353 Berlin, Germany.
LA  - eng
GR  - R01 AI50113-17/NIH/NIAID/
GR  - R03 AI119617/AI/NIAID NIH HHS/United States
GR  - R01 AI39115-24/NIH/NIAID/
PT  - Journal Article
DEP - 20220311
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC8949899
OTO - NOTNLM
OT  - fluorescence
OT  - hybridization
OT  - in situ
OT  - mucormycosis
OT  - pathology
COIS- The authors declare no conflict of interest.
EDAT- 2022/03/26 06:00
MHDA- 2022/03/26 06:01
PMCR- 2022/03/11
CRDT- 2022/03/25 01:12
PHST- 2022/02/18 00:00 [received]
PHST- 2022/03/05 00:00 [revised]
PHST- 2022/03/08 00:00 [accepted]
PHST- 2022/03/25 01:12 [entrez]
PHST- 2022/03/26 06:00 [pubmed]
PHST- 2022/03/26 06:01 [medline]
PHST- 2022/03/11 00:00 [pmc-release]
AID - jof8030289 [pii]
AID - jof-08-00289 [pii]
AID - 10.3390/jof8030289 [doi]
PST - epublish
SO  - J Fungi (Basel). 2022 Mar 11;8(3):289. doi: 10.3390/jof8030289.