PMID- 35333570
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20230403
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 8
IP  - 12
DP  - 2022 Mar 25
TI  - Structure and dynamics of Odinarchaeota tubulin and the implications for 
      eukaryotic microtubule evolution.
PG  - eabm2225
LID - 10.1126/sciadv.abm2225 [doi]
LID - eabm2225
AB  - Tubulins are critical for the internal organization of eukaryotic cells, and 
      understanding their emergence is an important question in eukaryogenesis. Asgard 
      archaea are the closest known prokaryotic relatives to eukaryotes. Here, we 
      elucidated the apo and nucleotide-bound x-ray structures of an Asgard tubulin 
      from hydrothermal living Odinarchaeota (OdinTubulin). The guanosine 
      5'-triphosphate (GTP)-bound structure resembles a microtubule protofilament, with 
      GTP bound between subunits, coordinating the "+" end subunit through a network of 
      water molecules and unexpectedly by two cations. A water molecule is located 
      suitable for GTP hydrolysis. Time course crystallography and electron microscopy 
      revealed conformational changes on GTP hydrolysis. OdinTubulin forms tubules at 
      high temperatures, with short curved protofilaments coiling around the tubule 
      circumference, more similar to FtsZ, rather than running parallel to its length, 
      as in microtubules. Thus, OdinTubulin represents an evolutionary stage 
      intermediate between prokaryotic FtsZ and eukaryotic microtubule-forming 
      tubulins.
FAU - Akil, Caner
AU  - Akil C
AUID- ORCID: 0000-0002-9464-1903
AD  - Research Institute for Interdisciplinary Science, Okayama University, Okayama 
      700-8530, Japan.
AD  - Tokyo Institute of Technology, Earth-Life Science Institute (ELSI), Tokyo 
      152-8551, Japan.
FAU - Ali, Samson
AU  - Ali S
AUID- ORCID: 0000-0002-1410-990X
AD  - Research Institute for Interdisciplinary Science, Okayama University, Okayama 
      700-8530, Japan.
AD  - Division of Biological Science, Graduate School of Science, Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
FAU - Tran, Linh T
AU  - Tran LT
AUID- ORCID: 0000-0003-0654-6517
AD  - Research Institute for Interdisciplinary Science, Okayama University, Okayama 
      700-8530, Japan.
FAU - Gaillard, Jeremie
AU  - Gaillard J
AUID- ORCID: 0000-0002-6943-8874
AD  - University of Grenoble-Alpes, CEA, CNRS, INRA, Interdisciplinary Research 
      Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Vegetale, 
      CytoMorpho Lab, 38054 Grenoble, France.
FAU - Li, Wenfei
AU  - Li W
AUID- ORCID: 0000-0003-2679-4075
AD  - National Laboratory of Solid State Microstructure, Department of Physics, 
      Collaborative Innovation Center of Advanced Microstructures, Nanjing University, 
      210093 Nanjing, China.
FAU - Hayashida, Kenichi
AU  - Hayashida K
AD  - Cellular and Structural Physiology Institute (CeSPI), Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
FAU - Hirose, Mika
AU  - Hirose M
AUID- ORCID: 0000-0001-8991-587X
AD  - Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
FAU - Kato, Takayuki
AU  - Kato T
AUID- ORCID: 0000-0002-8879-6685
AD  - Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
FAU - Oshima, Atsunori
AU  - Oshima A
AUID- ORCID: 0000-0003-1174-1368
AD  - Cellular and Structural Physiology Institute (CeSPI), Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
AD  - Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical 
      Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
AD  - Institute for Glyco-core Research (iGCORE), Nagoya University, Furo-cho, 
      Chikusa-ku, Nagoya 464-8601, Japan.
FAU - Fujishima, Kosuke
AU  - Fujishima K
AD  - Tokyo Institute of Technology, Earth-Life Science Institute (ELSI), Tokyo 
      152-8551, Japan.
AD  - Graduate School of Media and Governance, Keio University, Fujisawa 252-0882, 
      Japan.
FAU - Blanchoin, Laurent
AU  - Blanchoin L
AD  - University of Grenoble-Alpes, CEA, CNRS, INRA, Interdisciplinary Research 
      Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Vegetale, 
      CytoMorpho Lab, 38054 Grenoble, France.
AD  - Universite de Paris, INSERM, CEA, Institut de Recherche Saint Louis, U 976, 
      CytoMorpho Lab, 75010 Paris, France.
FAU - Narita, Akihiro
AU  - Narita A
AUID- ORCID: 0000-0003-1504-9352
AD  - Division of Biological Science, Graduate School of Science, Nagoya University, 
      Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
FAU - Robinson, Robert C
AU  - Robinson RC
AUID- ORCID: 0000-0001-6367-6903
AD  - Research Institute for Interdisciplinary Science, Okayama University, Okayama 
      700-8530, Japan.
AD  - School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of 
      Science and Technology (VISTEC), Rayong 21210, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20220325
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 0 (Tubulin)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - Eukaryota/metabolism
MH  - *Eukaryotic Cells/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Microtubules/metabolism
MH  - *Tubulin/chemistry
PMC - PMC8956254
EDAT- 2022/03/26 06:00
MHDA- 2022/04/07 06:00
PMCR- 2022/03/25
CRDT- 2022/03/25 17:17
PHST- 2022/03/25 17:17 [entrez]
PHST- 2022/03/26 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
PHST- 2022/03/25 00:00 [pmc-release]
AID - abm2225 [pii]
AID - 10.1126/sciadv.abm2225 [doi]
PST - ppublish
SO  - Sci Adv. 2022 Mar 25;8(12):eabm2225. doi: 10.1126/sciadv.abm2225. Epub 2022 Mar 
      25.