PMID- 35336858
OWN - NLM
STAT- MEDLINE
DCOM- 20220414
LR  - 20220414
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 3
DP  - 2022 Feb 22
TI  - Highly Pathogenic PRRSV-Infected Alveolar Macrophages Impair the Function of 
      Pulmonary Microvascular Endothelial Cells.
LID - 10.3390/v14030452 [doi]
LID - 452
AB  - The porcine reproductive and respiratory syndrome virus (PRRSV), especially the 
      highly pathogenic strains, can cause serious acute lung injury (ALI), 
      characterized by extensive hemorrhage, inflammatory cells and serous fluid 
      infiltration in the lung vascular system. Meanwhile, the pulmonary microvascular 
      endothelial cells (PMVECs) are essential for forming the air-blood barrier and 
      keeping the water-salt balance to prevent leakage of circulating nutrients, 
      solutes, and fluid into the underlying tissues. As well, they tightly regulate 
      the influx of immune cells. To determine the possible relationship between the 
      PMVECs' function changes and lung vascular permeability during PRRSV infection, 
      the PMVECs were co-cultured with HP-PRRSV-inoculated primary pulmonary alveolar 
      macrophages (PAMs) in transwell model, and then the RNA sequencing (RNA-seq) and 
      comprehensive bioinformatics analysis were carried out to characterize the 
      dynamic transcriptome landscapes of PMVECs. In total, 16,489 annotated genes were 
      identified, with 275 upregulated and 270 downregulated differentially expressed 
      genes (DEGs) were characterized at both 18 and 24 h post PRRSV inoculation. The 
      GO terms and KEGG pathways analysis indicated that the immune response, metabolic 
      pathways, cell death, cytokine-cytokine receptor interaction, viral responses, 
      and apoptotic process are significantly regulated upon co-culture with 
      PRRSV-infected PAMs. Moreover, according to the TERR and dextran flux assay 
      results, dysregulation of TJ proteins, including CLDN1, CLDN4, CLDN8, and OCLN, 
      is further confirmed to correlate with the increased permeability of PMVECs. 
      These transcriptome profiles and DEGs will provide valuable clues for further 
      exploring the roles of PMVECs in PRRSV-induced ALI in the future.
FAU - Sun, Weifeng
AU  - Sun W
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Wu, Weixin
AU  - Wu W
AUID- ORCID: 0000-0002-0734-3247
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Jiang, Nan
AU  - Jiang N
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Ge, Xinna
AU  - Ge X
AUID- ORCID: 0000-0002-5368-9687
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Zhang, Yongning
AU  - Zhang Y
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Han, Jun
AU  - Han J
AUID- ORCID: 0000-0002-7104-7223
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Guo, Xin
AU  - Guo X
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Zhou, Lei
AU  - Zhou L
AUID- ORCID: 0000-0002-8837-3965
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
FAU - Yang, Hanchun
AU  - Yang H
AUID- ORCID: 0000-0003-3359-3925
AD  - Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural 
      Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 
      100193, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220222
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
SB  - IM
MH  - Animals
MH  - Endothelial Cells
MH  - Lung/pathology
MH  - Macrophages, Alveolar
MH  - *Porcine Reproductive and Respiratory Syndrome/metabolism
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
PMC - PMC8948932
OTO - NOTNLM
OT  - cytokines
OT  - porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - pulmonary microvascular endothelial cells (PMVECs)
OT  - tight junctions (TJs)
OT  - transcriptome analysis
OT  - transwell co-cultures
COIS- The authors declare no conflict of interest.
EDAT- 2022/03/27 06:00
MHDA- 2022/04/15 06:00
PMCR- 2022/02/22
CRDT- 2022/03/26 01:07
PHST- 2022/01/28 00:00 [received]
PHST- 2022/02/16 00:00 [revised]
PHST- 2022/02/17 00:00 [accepted]
PHST- 2022/03/26 01:07 [entrez]
PHST- 2022/03/27 06:00 [pubmed]
PHST- 2022/04/15 06:00 [medline]
PHST- 2022/02/22 00:00 [pmc-release]
AID - v14030452 [pii]
AID - viruses-14-00452 [pii]
AID - 10.3390/v14030452 [doi]
PST - epublish
SO  - Viruses. 2022 Feb 22;14(3):452. doi: 10.3390/v14030452.