PMID- 35339043
OWN - NLM
STAT- MEDLINE
DCOM- 20220414
LR  - 20220531
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 153
DP  - 2022 May
TI  - Recombinant guanosine-5'-triphosphate (GTP)-binding protein associated with 
      Poloxamer 407-based polymeric micelles protects against Leishmania infantum 
      infection.
PG  - 155865
LID - S1043-4666(22)00074-6 [pii]
LID - 10.1016/j.cyto.2022.155865 [doi]
AB  - Leishmania virulence proteins should be considered as vaccine candidates against 
      disease, since they are involved in developing infection in mammalian hosts. In a 
      previous study, a Leishmania guanosine-5'-triphosphate (GTP)-binding protein was 
      identified as a potential parasite virulence factor. In the present work, the 
      gene encoding GTP was cloned and the recombinant protein (rGTP) was evaluated as 
      a vaccine candidate against Leishmania infantum infection. The protein was 
      associated with saponin (rGTP/Sap) or Poloxamer 407-based micelles (rGTP/Mic) as 
      adjuvants, and protective efficacy was investigated in BALB/c mice after parasite 
      challenge. Both rGTP/Sap and rGTP/Mic compositions induced a Th1-type immune 
      response in vaccinated animals, with significantly higher levels of IFN-gamma, IL-12, 
      IL-2, TNF-alpha, GM-CSF, nitrite, specific IgG2a isotype antibody and positive 
      lymphoproliferation, when compared to the control groups. This response was 
      accompanied by significantly lower parasite load in the spleens, livers, bone 
      marrows and draining lymph nodes of the animals. Immunological and 
      parasitological evaluations indicated that rGTP/Mic induced a more polarized 
      Th1-type response and higher reduction in the organ parasitism, and with lower 
      hepatotoxicity, when compared to the use of rGTP/Sap. In conclusion, our 
      preliminary data suggest that rGTP could be considered for further development as 
      a vaccine candidate to protect against VL.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Lage, Daniela P
AU  - Lage DP
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Machado, Amanda S
AU  - Machado AS
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Vale, Danniele L
AU  - Vale DL
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Freitas, Camila S
AU  - Freitas CS
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Linhares, Flavia P
AU  - Linhares FP
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Cardoso, Jamille M O
AU  - Cardoso JMO
AD  - Laboratorio de Imunopatologia, Nucleo de Pesquisas em Ciencias Biologicas/NUPEB, 
      Departamento de Ciencias Biologicas, Insituto de Ciencias Exatas e Biologicas, 
      Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
FAU - Pereira, Isabela A G
AU  - Pereira IAG
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Ramos, Fernanda F
AU  - Ramos FF
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Tavares, Grasiele S V
AU  - Tavares GSV
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Ludolf, Fernanda
AU  - Ludolf F
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Oliveira-da-Silva, Joao A
AU  - Oliveira-da-Silva JA
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Bandeira, Raquel S
AU  - Bandeira RS
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Silva, Alessandra M
AU  - Silva AM
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Simoes, Luciana C
AU  - Simoes LC
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Reis, Thiago A R
AU  - Reis TAR
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Oliveira, Jamil S
AU  - Oliveira JS
AD  - Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, Belo Horizonte, 31270-901 Minas Gerais, 
      Brazil.
FAU - Christodoulides, Myron
AU  - Christodoulides M
AD  - Neisseria Research Group, Molecular Microbiology, School of Clinical and 
      Experimental Sciences, University of Southampton Faculty of Medicine, Southampton 
      General Hospital, Southampton SO16 6YD, England.
FAU - Chavez-Fumagalli, Miguel A
AU  - Chavez-Fumagalli MA
AD  - Universidad Catolica de Santa Maria, Urb. San Jose S/N, Umacollo, Arequipa, Peru.
FAU - Roatt, Bruno M
AU  - Roatt BM
AD  - Laboratorio de Imunopatologia, Nucleo de Pesquisas em Ciencias Biologicas/NUPEB, 
      Departamento de Ciencias Biologicas, Insituto de Ciencias Exatas e Biologicas, 
      Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
FAU - Martins, Vivian T
AU  - Martins VT
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
FAU - Coelho, Eduardo A F
AU  - Coelho EAF
AD  - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, 
      Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Prof. Alfredo 
      Balena, 190, 30130-100, Belo Horizonte, Minas Gerais, Brazil; Departamento de 
      Patologia Clinica, COLTEC, Universidade Federal de Minas Gerais, Av. Antonio 
      Carlos, 6627, 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: 
      eduardoferrazcoelho@yahoo.com.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220323
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Carrier Proteins)
RN  - 0 (Micelles)
RN  - 0 (Polyphosphates)
RN  - 0 (Recombinant Proteins)
RN  - 106392-12-5 (Poloxamer)
RN  - 12133JR80S (Guanosine)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - NU43IAG5BC (triphosphoric acid)
SB  - IM
MH  - Animals
MH  - Antigens, Protozoan
MH  - Carrier Proteins
MH  - Guanosine
MH  - Guanosine Triphosphate
MH  - *Leishmania infantum
MH  - *Leishmaniasis
MH  - *Leishmaniasis, Visceral
MH  - Mammals
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Micelles
MH  - Poloxamer
MH  - Polyphosphates
MH  - Recombinant Proteins
OTO - NOTNLM
OT  - GTP-binding protein
OT  - Immune response
OT  - Micelle
OT  - Saponin
OT  - Vaccine
OT  - Visceral leishmaniasis
EDAT- 2022/03/27 06:00
MHDA- 2022/04/15 06:00
CRDT- 2022/03/26 20:11
PHST- 2021/11/05 00:00 [received]
PHST- 2022/02/12 00:00 [revised]
PHST- 2022/03/09 00:00 [accepted]
PHST- 2022/03/27 06:00 [pubmed]
PHST- 2022/04/15 06:00 [medline]
PHST- 2022/03/26 20:11 [entrez]
AID - S1043-4666(22)00074-6 [pii]
AID - 10.1016/j.cyto.2022.155865 [doi]
PST - ppublish
SO  - Cytokine. 2022 May;153:155865. doi: 10.1016/j.cyto.2022.155865. Epub 2022 Mar 23.