PMID- 35339759
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220616
IS  - 1980-5322 (Electronic)
IS  - 1807-5932 (Print)
IS  - 1807-5932 (Linking)
VI  - 77
DP  - 2022
TI  - microRNA-181a-5p impedes the proliferation, migration, and invasion of 
      retinoblastoma cells by targeting the NRAS proto-oncogene.
PG  - 100026
LID - S1807-5932(22)00022-9 [pii]
LID - 10.1016/j.clinsp.2022.100026 [doi]
LID - 100026
AB  - OBJECTIVES: Accumulating research have reported that microRNAs (miRNAs) play 
      important roles in Retinoblastoma (RB). Nonetheless, the function and underlying 
      mechanism of miR-181a-5p in RB remain ambiguous. METHODS: The relative expression 
      levels of miR-181a-5p and NRAS mRNA were detected by quantitative Reverse 
      Transcription-Polymerase Chain Reaction (qRT-PCR). RB cell proliferation was 
      measured using the Cell Counting Kit-8 (CCK-8) and 5'-Bromo-2'-deoxyuridine 
      (BrdU) assays. Transwell assays and flow cytometry were performed to detect the 
      migration, invasion, and apoptosis of RB cells. The interaction between 
      miR-181a-5p and NRAS was explored using luciferase experiments, western blotting, 
      and qRT-PCR. RESULTS: miR-181a-5p expression was found to be decreased in RB 
      tissues and cell lines, and its expression was correlated with unfavorable 
      pathological features of the patients. In vitro experiments revealed that 
      miR-181a-5p reduced RB cell proliferation, migration, and invasion while 
      enhancing apoptosis. Further research confirmed that NRAS is a direct target of 
      miR-181a-5p. miR-181a-5p inhibited NRAS expression at both the mRNA and protein 
      levels. Co-transfection of pcDNA-NRAS or NRAS small interfering RNA (siRNA) 
      reversed the effects of miR-181a-5p mimics or miR-181a-5p inhibitors on RB cells. 
      CONCLUSION: miR-181a-5p was significantly downregulated during the development of 
      RB, and it suppressed the malignant behaviors of RB cells by targeting NRAS.
CI  - Copyright (c) 2022 HCFMUSP. Published by Elsevier Espana, S.L.U. All rights 
      reserved.
FAU - Ouyang, Ming
AU  - Ouyang M
AD  - Shenzhen Eye Hospital, Jinan University, China. Electronic address: 
      taohe792@163.com.
FAU - Liu, Guiqin
AU  - Liu G
AD  - Shenzhen Eye Hospital, Jinan University, China.
FAU - Xiong, Cheng
AU  - Xiong C
AD  - Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, School of 
      Optometry, Shenzhen University, China.
FAU - Rao, Jing
AU  - Rao J
AD  - Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, School of 
      Optometry, Shenzhen University, China.
LA  - eng
PT  - Journal Article
DEP - 20220324
PL  - United States
TA  - Clinics (Sao Paulo)
JT  - Clinics (Sao Paulo, Brazil)
JID - 101244734
RN  - 0 (MIrn181 microRNA, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (NRAS protein, human)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - GTP Phosphohydrolases/genetics
MH  - Humans
MH  - Membrane Proteins/genetics
MH  - *MicroRNAs/genetics
MH  - Neoplasm Invasiveness/genetics
MH  - Proto-Oncogenes
MH  - *Retinal Neoplasms/genetics
MH  - *Retinoblastoma/genetics/pathology
PMC - PMC8961171
OTO - NOTNLM
OT  - NRAS
OT  - Retinoblastoma
OT  - miR-181a-5p
COIS- Conflicts of interest The authors declare no conflicts of interest.
EDAT- 2022/03/28 06:00
MHDA- 2022/04/05 06:00
PMCR- 2022/03/24
CRDT- 2022/03/27 20:22
PHST- 2021/05/19 00:00 [received]
PHST- 2021/08/16 00:00 [accepted]
PHST- 2022/03/27 20:22 [entrez]
PHST- 2022/03/28 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2022/03/24 00:00 [pmc-release]
AID - S1807-5932(22)00022-9 [pii]
AID - 100026 [pii]
AID - 10.1016/j.clinsp.2022.100026 [doi]
PST - epublish
SO  - Clinics (Sao Paulo). 2022 Mar 24;77:100026. doi: 10.1016/j.clinsp.2022.100026. 
      eCollection 2022.