PMID- 35340587
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220405
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2022
DP  - 2022
TI  - Ebosin Attenuates the Inflammatory Responses Induced by TNF-alpha through Inhibiting 
      NF-kappaB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes.
PG  - 9166370
LID - 10.1155/2022/9166370 [doi]
LID - 9166370
AB  - Tumor necrosis factor-alpha (TNF-alpha) lies at the apex of signal transduction cascades 
      that results in induced destruction of joints in rheumatoid arthritis. It is 
      therefore of great medicinal interest to modulate the cellular responses to 
      TNF-alpha. Ebosin, a novel exopolysaccharide derived from Streptomyces sp, has been 
      demonstrated to have remarkable therapeutic actions on collagen-induced arthritis 
      in rats, while it also suppressed the production of IL-1beta, TNF-alpha, and IL-6 at 
      both mRNA and protein levels in cultured fibroblast-like synoviocytes. In order 
      to further understand the potential mechanisms involved in the anti-inflammatory 
      effects of ebosin at molecular level, we investigated the impact of it on the 
      activation of MAPK and NF-kappaB pathways following TNF-alpha induced in fibroblast-like 
      synoviocytes (FLS). The results showed that the phosphorylation levels of 
      TNF-alpha-induced p38, JNK1, JNK2, IKKalpha, IKKbeta, and IkappaB, as well as NF-kappaB nuclear 
      translocation, were reduced significantly in FLS cells in response to ebosin. 
      Furthermore, we proved that ebosin decreased the level of NF-kappaB in the nucleus 
      and blocked the DNA-binding ability of NF-kappaB using electrophoresis mobility gel 
      shift assay. Besides, low levels of matrix metalloproteinases (MMP-1 and MMP-3) 
      and chemokines (interleukin-8 and RANTES) were found in TNF-alpha-stimulated 
      fibroblast-like synoviocytes treated with ebosin. These results indicate that 
      ebosin can suppress a range of activities in both MAPK and NF-kappaB pathways induced 
      by TNF-alpha in rat fibroblast-like synoviocytes, which provides a rationale for 
      examining the use of ebosin as a potential therapeutic candidate for rheumatic 
      arthritis.
CI  - Copyright (c) 2022 Yang Zhang et al.
FAU - Zhang, Yang
AU  - Zhang Y
AUID- ORCID: 0000-0001-7462-2935
AD  - NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of 
      Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Tiantan 
      Xili, 100050 Beijing, China.
AD  - Beijing Institute of Hepatology, Beijing Youan Hospital, Capital Medical 
      University, Beijing 100069, China.
FAU - Wang, Lifei
AU  - Wang L
AD  - NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of 
      Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Tiantan 
      Xili, 100050 Beijing, China.
FAU - Bai, Liping
AU  - Bai L
AD  - NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of 
      Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Tiantan 
      Xili, 100050 Beijing, China.
FAU - Jiang, Rong
AU  - Jiang R
AD  - NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of 
      Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Tiantan 
      Xili, 100050 Beijing, China.
FAU - Wu, Jianbo
AU  - Wu J
AD  - NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of 
      Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Tiantan 
      Xili, 100050 Beijing, China.
FAU - Li, Yuan
AU  - Li Y
AUID- ORCID: 0000-0001-7110-4622
AD  - NHC Key Laboratory of Biotechnology of Antibiotics, CAMS Key Laboratory of 
      Synthetic Biology for Drug Innovation, Institute of Medicinal Biotechnology, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Tiantan 
      Xili, 100050 Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20220317
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (NF-kappa B)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (ebosin)
SB  - IM
MH  - Animals
MH  - Fibroblasts
MH  - *NF-kappa B/metabolism
MH  - Polysaccharides, Bacterial
MH  - Rats
MH  - *Synoviocytes/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC8947919
COIS- All authors declare no conflict of interest.
EDAT- 2022/03/29 06:00
MHDA- 2022/04/05 06:00
PMCR- 2022/03/17
CRDT- 2022/03/28 05:11
PHST- 2021/07/06 00:00 [received]
PHST- 2021/12/04 00:00 [revised]
PHST- 2022/01/22 00:00 [accepted]
PHST- 2022/03/28 05:11 [entrez]
PHST- 2022/03/29 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2022/03/17 00:00 [pmc-release]
AID - 10.1155/2022/9166370 [doi]
PST - epublish
SO  - J Immunol Res. 2022 Mar 17;2022:9166370. doi: 10.1155/2022/9166370. eCollection 
      2022.