PMID- 35345861
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220330
IS  - 2214-7500 (Electronic)
IS  - 2214-7500 (Linking)
VI  - 9
DP  - 2022
TI  - Local lymph node assay: 5-bromo-d-deoxyuridine-ELISA method for comparative study 
      in assessing chemical potencies and skin sensitization in BALB/c and CBA/J 
      strains.
PG  - 499-504
LID - 10.1016/j.toxrep.2022.03.017 [doi]
AB  - Local lymph node assay (LLNA) is a predictive in vivo method to provide estimates 
      of relative potency and to contribute to risk assessment/risk management 
      regarding skin sensitizing potency of chemicals and formulations as a stand-alone 
      alternative test. In addition, LLNA is relatively rapid and cost-effective 
      compared to the Buehler method (Guinea pig test), and confers important animal 
      welfare benefits. CBA/J and BALB/c strains are widely commercially available and 
      have been evaluated by formal LLNA validation studies. However, the LLNA method 
      using BrdU with ELISA, unlike other LLNA methods (OECD TG 429, 442 A, 442B), has 
      not been previously validated. Therefore, in this study a validation method was 
      performed to evaluate if the LLNA:BrdU-ELISA method could also be used to 
      identify sensitizers among chemicals listed in OECD TG 429 using CBA/J and BALB/c 
      strains. Here, we newly found that the LLNA:BrdU-ELISA validation method 
      correctly identified 12 of 13 sensitizers in the BALB/c, 11 of 13 sensitizers in 
      the CBA/J, and 3 of 5 non-sensitizers were identified in the two strains. 
      Collectively, we found that the results of LLNA:BrdU-ELISA method provide a 
      similar level of performance for accuracy and sensitivity in two mouse strains 
      BALB/c and CBA/J.
CI  - (c) 2022 The Authors.
FAU - Oh, Jeong-Ja
AU  - Oh JJ
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Park, Eun-Sung
AU  - Park ES
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Kim, Seong-Sook
AU  - Kim SS
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Lee, Min-Seung
AU  - Lee MS
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Lee, Woo-Joo
AU  - Lee WJ
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Pak, Bum-Su
AU  - Pak BS
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Kim, Min-Sub
AU  - Kim MS
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Lee, Hyun-Kul
AU  - Lee HK
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Song, Si-Whan
AU  - Song SW
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
FAU - Kim, Sun-Don
AU  - Kim SD
AD  - Nonclinical Research Institute, Chemon Inc., 240, Nampyeongro, Yangi-Myeon, 
      Cheoin-Gu, Yongin-Si, Gyeonggi-Do, 17162, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20220321
PL  - Ireland
TA  - Toxicol Rep
JT  - Toxicology reports
JID - 101630272
PMC - PMC8956910
OTO - NOTNLM
OT  - BALB/c
OT  - CBA/J
OT  - LLNA:BrdU-ELISA method
OT  - Local lymph node assay
OT  - Skin sensitization
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/03/30 06:00
MHDA- 2022/03/30 06:01
PMCR- 2022/03/21
CRDT- 2022/03/29 05:15
PHST- 2021/09/10 00:00 [received]
PHST- 2022/03/10 00:00 [revised]
PHST- 2022/03/17 00:00 [accepted]
PHST- 2022/03/29 05:15 [entrez]
PHST- 2022/03/30 06:00 [pubmed]
PHST- 2022/03/30 06:01 [medline]
PHST- 2022/03/21 00:00 [pmc-release]
AID - S2214-7500(22)00046-4 [pii]
AID - 10.1016/j.toxrep.2022.03.017 [doi]
PST - epublish
SO  - Toxicol Rep. 2022 Mar 21;9:499-504. doi: 10.1016/j.toxrep.2022.03.017. 
      eCollection 2022.