PMID- 35354542
OWN - NLM
STAT- MEDLINE
DCOM- 20221102
LR  - 20221228
IS  - 1878-3562 (Electronic)
IS  - 1590-8658 (Linking)
VI  - 54
IP  - 11
DP  - 2022 Nov
TI  - Long noncoding RNA SNHG8 promotes chemoresistance in gastric cancer via binding 
      with hnRNPA1 and stabilizing TROY expression.
PG  - 1573-1582
LID - S1590-8658(22)00202-X [pii]
LID - 10.1016/j.dld.2022.02.011 [doi]
AB  - AIMS: To determine SNHG8's function and potential mechanisms in gastric cancer 
      (GC) chemoresistance. METHODS: We assessed SNHG8 expression in GC cell lines, 
      GC/CDDP cell lines (cell lines treated with cisplatin), and 42 GC tissues and 
      SNHG8 levels in the lncRNA microarray analysis of AGS/CDDP and AGS cell lines. We 
      also examined GC cell viability in vivo and in vitro and its apoptosis level with 
      Flow cytometry assays. SNHG8 was localized in subcells using fluorescence in situ 
      hybridization (FISH) and cell fraction assays, hnRNPA1's link to SNHG8 was 
      determined utilizing RNA immunoprecipitation (RIP) and FISH assays, gene 
      expression profiles were assessed employing RNA transcriptome sequencing, and 
      hnRNPA1's relationship with TROY was ascertained with the RIP assay. RESULTS: 
      SNHG8 increased significantly in GC cell lines and GC tissues. However, a 
      decrease in its expression promoted sensitivity to chemotherapy and inhibited DNA 
      damage repair in vitro and in vivo. SNHG8 appeared to regulate TROY expression 
      via linking with hnRNPA1. Reducing TROY levels considerably stimulated GC cell 
      chemosensitivity, whereas heightening them partially rescued the rate of 
      chemoresistance caused by downregulating SNHG8. CONCLUSION: In summary, the 
      "SNHG8/hnRNPA1-TROY" axis is crucial to GC chemoresistance.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Zhu, Wenzhong
AU  - Zhu W
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430022, China.
FAU - Tan, Lulu
AU  - Tan L
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430022, China.
FAU - Ma, Tiantian
AU  - Ma T
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430022, China.
FAU - Yin, Zhijie
AU  - Yin Z
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430022, China.
FAU - Gao, Jinbo
AU  - Gao J
AD  - Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, 430022, China. Electronic 
      address: jgao@hust.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220327
PL  - Netherlands
TA  - Dig Liver Dis
JT  - Digestive and liver disease : official journal of the Italian Society of 
      Gastroenterology and the Italian Association for the Study of the Liver
JID - 100958385
RN  - Q20Q21Q62J (Cisplatin)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (hnRNPA1 protein, human)
RN  - 0 (Heterogeneous Nuclear Ribonucleoprotein A1)
RN  - 0 (TNFRSF19 protein, human)
RN  - 0 (Receptors, Tumor Necrosis Factor)
SB  - IM
MH  - Humans
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cisplatin/pharmacology/therapeutic use
MH  - Drug Resistance, Neoplasm/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Stomach Neoplasms/drug therapy/genetics/metabolism
MH  - *Heterogeneous Nuclear Ribonucleoprotein A1/metabolism
MH  - *Receptors, Tumor Necrosis Factor/metabolism
OTO - NOTNLM
OT  - Chemoresistance
OT  - Gastric cancer
OT  - SNHG8
OT  - TROY
EDAT- 2022/04/01 06:00
MHDA- 2022/11/02 06:00
CRDT- 2022/03/31 05:10
PHST- 2021/09/14 00:00 [received]
PHST- 2022/02/21 00:00 [revised]
PHST- 2022/02/22 00:00 [accepted]
PHST- 2022/04/01 06:00 [pubmed]
PHST- 2022/11/02 06:00 [medline]
PHST- 2022/03/31 05:10 [entrez]
AID - S1590-8658(22)00202-X [pii]
AID - 10.1016/j.dld.2022.02.011 [doi]
PST - ppublish
SO  - Dig Liver Dis. 2022 Nov;54(11):1573-1582. doi: 10.1016/j.dld.2022.02.011. Epub 
      2022 Mar 27.