PMID- 35354714
OWN - NLM
STAT- MEDLINE
DCOM- 20220809
LR  - 20220826
IS  - 1881-7122 (Electronic)
IS  - 1341-1357 (Print)
IS  - 0007-5124 (Linking)
VI  - 71
IP  - 3
DP  - 2022 Aug 5
TI  - A 3-Mbp fragment on rat chromosome 1 affects susceptibility both to stroke and 
      kidney injury under salt loading in the stroke-prone spontaneously hypertensive 
      rat: a genetic approach using multiple congenic strains.
PG  - 368-375
LID - 10.1538/expanim.21-0189 [doi]
AB  - We have previously reported that a major quantitative trait locus (QTL) 
      responsible for susceptibility to salt-induced stroke in the stroke-prone 
      spontaneously hypertensive rat (SHRSP) is located in a 3-Mbp region on chromosome 
      1 covered by SHRSP.SHR-(D1Rat23-D1Rat213)/Izm (termed Pr1.31), a congenic strain 
      with segments from SHRSP/Izm introduced into the stroke-resistant SHR/Izm. Here, 
      we attempted to narrow down the candidate region on chromosome 1 further through 
      analyses of subcongenic strains constructed for the target region. 
      Simultaneously, salt-induced kidney injury was evaluated through the measurement 
      of urinary albumin and the gene expression of renal tubular injury markers (Kim-1 
      and Clu) to explore a possible mechanism leading to the onset of stroke. All 
      subcongenic strains examined in this study showed lower susceptibility to 
      salt-induced stroke than SHRSP. Interestingly, Pr1.31 had the lowest stroke 
      susceptibility when compared with newly constructed subcongenic strains harboring 
      fragments of the congenic sequence in Pr1.31. Although Kim-1 and Clu expression 
      after 1 week of salt loading in Pr1.31 did not differ significantly from those in 
      SHRSP, the urinary albumin level of Pr1.31 was significantly lower than those of 
      the other subcongenic strains and that of SHRSP. The present results indicated 
      that, although the congenic fragment in Pr1.31 harbored the gene(s) related to 
      salt-induced organ damages, further genetic dissection of the candidate region 
      was difficult due to multiple QTLs suggested in this region. Further analysis 
      using Pr1.31 will unveil genetic and pathophysiological mechanisms underlying 
      salt-induced end organ damages in SHRSP.
FAU - Wang, Mei
AU  - Wang M
AD  - Department of Functional Pathology, Faculty of Medicine, Shimane University, 89-1 
      Enya-cho, Izumo, Shimane 693-8501, Japan.
AD  - Graduate School, Ningxia Medical University, 1160 Shengli Street, Xingqing 
      District, Yinchuan, Ningxia 750004, P.R. China.
AD  - Department of Cardiology, Shanghai Gongli Hospital, Second Military Medical 
      University, 207 Juye Road, Pudong, Shanghai 200135, P.R. China.
FAU - Ohara, Hiroki
AU  - Ohara H
AD  - Department of Functional Pathology, Faculty of Medicine, Shimane University, 89-1 
      Enya-cho, Izumo, Shimane 693-8501, Japan.
FAU - Egawa, Masahiro
AU  - Egawa M
AD  - Division of Nephrology, Shimane University Hospital, 89-1 Enya-cho, Izumo, 
      Shimane 693-8501, Japan.
FAU - Fukunaga, Shohei
AU  - Fukunaga S
AD  - Division of Nephrology, Shimane University Hospital, 89-1 Enya-cho, Izumo, 
      Shimane 693-8501, Japan.
FAU - Matsuo, Hiroyuki
AU  - Matsuo H
AD  - Department of Functional Pathology, Faculty of Medicine, Shimane University, 89-1 
      Enya-cho, Izumo, Shimane 693-8501, Japan.
AD  - Department of Experimental Animals, Interdisciplinary Center for Science 
      Research, Head Office for Research and Academic Information, Shimane University, 
      89-1 Enya-cho, Izumo, Shimane 693-8501, Japan.
FAU - Ge, Zhi-Ru
AU  - Ge ZR
AD  - Department of Cardiology, Shanghai Gongli Hospital, Second Military Medical 
      University, 207 Juye Road, Pudong, Shanghai 200135, P.R. China.
FAU - Nabika, Toru
AU  - Nabika T
AD  - Department of Functional Pathology, Faculty of Medicine, Shimane University, 89-1 
      Enya-cho, Izumo, Shimane 693-8501, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220329
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 0 (Albumins)
RN  - 0 (Sodium Chloride, Dietary)
SB  - IM
MH  - Albumins/adverse effects/genetics
MH  - Animals
MH  - Humans
MH  - *Hypertension/genetics
MH  - Kidney
MH  - Rats
MH  - Rats, Inbred SHR
MH  - *Sodium Chloride, Dietary/adverse effects
MH  - *Stroke/genetics
PMC - PMC9388333
OTO - NOTNLM
OT  - congenic strain
OT  - quantitative trait loci (QTL)
OT  - spontaneously hypertensive rat (SHR)
OT  - stroke-prone SHR (SHRSP)
COIS- The authors declare no conflicts of interest.
EDAT- 2022/04/01 06:00
MHDA- 2022/08/10 06:00
PMCR- 2022/01/01
CRDT- 2022/03/31 05:11
PHST- 2022/04/01 06:00 [pubmed]
PHST- 2022/08/10 06:00 [medline]
PHST- 2022/03/31 05:11 [entrez]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 21-0189 [pii]
AID - 10.1538/expanim.21-0189 [doi]
PST - ppublish
SO  - Exp Anim. 2022 Aug 5;71(3):368-375. doi: 10.1538/expanim.21-0189. Epub 2022 Mar 
      29.