PMID- 35355558
OWN - NLM
STAT- MEDLINE
DCOM- 20220401
LR  - 20220520
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - Mechanism of Endoplasmic Reticulum Stress Pathway in the Osteogenic Phenotypic 
      Transformation of Aortic Valve Interstitial Cells.
PG  - 856331
LID - 10.3389/fendo.2022.856331 [doi]
LID - 856331
AB  - BACKGROUND AND PURPOSE: Calcific Aortic Valve Disease (CAVD) is a crucial 
      component of degenerative valvular disease in old age and with the increasing 
      prevalence of the aging population. we hope that by modeling valvular 
      osteogenesis and intervening with endoplasmic reticulum stress inhibitor TUDCA to 
      observe the effect of endoplasmic reticulum stress on valve osteogenesis. 
      METHODS: In this study, rabbit heart valvular interstitial cells (VICs) were 
      isolated and cultured. They treated with ox-LDL (Oxidized Low Density 
      Lipoprotein) stimulation to establish a model of valvular osteogenic 
      transformation. BMP2 (Bone Morphogenetic Protein 2), PERK (Protein kinase R-like 
      endoplasmic reticulum kinase), CHOP (CCAAT/enhancer-binding protein homologous 
      protein) and transcriptional regulatory factor ATF4 (Activating Transcription 
      Factor 4 )were recorded after intervention with ER stress inhibitor TUDCA. The 
      effects of er stress on valvular osteogenic transformation were analyzed. RESULT: 
      After stimulation of VICs with ox-LDL, the expression levels of BMP2, PERK, CHOP, 
      and ATF4 increased. However, TUDCA treatment can alleviate the increased 
      expression levels of BMP2, PERK ATF4, and CHOP under ox-LDL stimulation to a 
      certain extent. CONCLUSION: The endoplasmic reticulum stress signaling pathway is 
      involved in ox-LDL-induced calcification of rabbit valve interstitial cells. 
      Inhibition of endoplasmic reticulum stress using TUDCA can improve the 
      progression of rabbit aortic valve calcification.
CI  - Copyright (c) 2022 Tao, Geng, Dang, Xu, Zhao, Zou and Li.
FAU - Tao, Yiming
AU  - Tao Y
AD  - Department of Intensive Care Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
AD  - The Emergency Department, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Geng, Yimin
AU  - Geng Y
AD  - Department of Intensive Care Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
AD  - The Emergency Department, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Dang, Wenpei
AU  - Dang W
AD  - Department of Intensive Care Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
AD  - The Emergency Department, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Xu, Xinxin
AU  - Xu X
AD  - Department of Intensive Care Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
AD  - The Emergency Department, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Zhao, Hui
AU  - Zhao H
AD  - Department of Intensive Care Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
AD  - The Emergency Department, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Zou, Lijuan
AU  - Zou L
AD  - Department of Intensive Care Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
AD  - The Emergency Department, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Li, Yongsheng
AU  - Li Y
AD  - Department of Intensive Care Medicine, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, China.
AD  - The Emergency Department, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20220308
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
SB  - IM
MH  - Animals
MH  - Aortic Valve/metabolism
MH  - *Aortic Valve Stenosis/metabolism
MH  - *Calcinosis
MH  - Cells, Cultured
MH  - Endoplasmic Reticulum Stress
MH  - Osteogenesis
MH  - Rabbits
PMC - PMC8959129
OTO - NOTNLM
OT  - BMP2
OT  - TUDCA
OT  - aortic valve calcification
OT  - endoplasmic reticulum stress
OT  - ox-LDL
OT  - valve interstitial cells
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/01 06:00
MHDA- 2022/04/02 06:00
PMCR- 2022/01/01
CRDT- 2022/03/31 05:18
PHST- 2022/01/17 00:00 [received]
PHST- 2022/02/07 00:00 [accepted]
PHST- 2022/03/31 05:18 [entrez]
PHST- 2022/04/01 06:00 [pubmed]
PHST- 2022/04/02 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.856331 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Mar 8;13:856331. doi: 
      10.3389/fendo.2022.856331. eCollection 2022.