PMID- 35356645
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240512
IS  - 2214-7500 (Electronic)
IS  - 2214-7500 (Linking)
VI  - 9
DP  - 2022
TI  - Enhanced sensitivity of an Ah-receptor system in yeast through condition 
      modification and use of mammalian modifiers.
PG  - 513-520
LID - 10.1016/j.toxrep.2022.03.012 [doi]
AB  - Proteins, such as the Ah receptor (AHR), hold potential as sensors to detect 
      ligands in environmental and biological samples, and may also serve as tools to 
      regulate biosynthetic and industrial processes. The AHR is also a prototype 
      system for the PAS superfamily that can sense and mediate adaptation to signals 
      as diverse as light, voltage, oxygen and an array of small molecules. The yeast, 
      S. cerevisiae, has proven to be an important model to study the signal 
      transduction of sensors like the AHR because of its ease of use, numerous 
      available strategies for genetic manipulation, and capacity for heterologous 
      expression. To better understand the utility of sensor proteins as components of 
      yeast detection systems, we characterized a chimeric AHR-LexA system that drives 
      expression from a Lex operator (LexO) driven, beta-galactosidase (beta-Gal) 
      reporter. In this report, we demonstrate that improvements in assays sensitivity 
      and pharmacology can arise from the careful optimization of yeast growth phase 
      and the duration of ligand exposure. We also report that the coexpression of 
      heterotypic modifiers from mammalian cells (e.g., the ARA9 and ARA3 proteins), 
      can improve yeast assay performance. We propose that complementing these assay 
      improvements with previously reported yeast mutations described by others will 
      expand the utility of the AHR for biotechnology applications.
FAU - Vazquez-Rivera, Emmanuel
AU  - Vazquez-Rivera E
AD  - Molecular and Environmental Toxicology Center, McArdle Laboratory for Cancer 
      Research, University of Wisconsin School of Medicine and Public Health, Madison, 
      WI 53706, USA.
FAU - Rojas, Brenda L
AU  - Rojas BL
AD  - Molecular and Environmental Toxicology Center, McArdle Laboratory for Cancer 
      Research, University of Wisconsin School of Medicine and Public Health, Madison, 
      WI 53706, USA.
FAU - Carney, Patrick R
AU  - Carney PR
AD  - Molecular and Environmental Toxicology Center, McArdle Laboratory for Cancer 
      Research, University of Wisconsin School of Medicine and Public Health, Madison, 
      WI 53706, USA.
FAU - Marrero-Valentin, Jose L
AU  - Marrero-Valentin JL
AD  - Molecular and Environmental Toxicology Center, McArdle Laboratory for Cancer 
      Research, University of Wisconsin School of Medicine and Public Health, Madison, 
      WI 53706, USA.
FAU - Bradfield, Christopher A
AU  - Bradfield CA
AD  - Molecular and Environmental Toxicology Center, McArdle Laboratory for Cancer 
      Research, University of Wisconsin School of Medicine and Public Health, Madison, 
      WI 53706, USA.
LA  - eng
GR  - R35 ES028377/ES/NIEHS NIH HHS/United States
GR  - P30 CA014520/CA/NCI NIH HHS/United States
GR  - T32 GM008692/GM/NIGMS NIH HHS/United States
GR  - R25 ES020720/ES/NIEHS NIH HHS/United States
GR  - T32 GM140935/GM/NIGMS NIH HHS/United States
GR  - T32 CA009135/CA/NCI NIH HHS/United States
GR  - T32 ES007015/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20220317
PL  - Ireland
TA  - Toxicol Rep
JT  - Toxicology reports
JID - 101630272
PMC - PMC8958262
OTO - NOTNLM
OT  - Ah receptor
OT  - Assay
OT  - Ligands
OT  - Polycyclic aromatic hydrocarbons
OT  - Reporter
OT  - Yeast
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/04/01 06:00
MHDA- 2022/04/01 06:01
PMCR- 2022/03/17
CRDT- 2022/03/31 05:35
PHST- 2021/12/15 00:00 [received]
PHST- 2022/03/03 00:00 [revised]
PHST- 2022/03/04 00:00 [accepted]
PHST- 2022/03/31 05:35 [entrez]
PHST- 2022/04/01 06:00 [pubmed]
PHST- 2022/04/01 06:01 [medline]
PHST- 2022/03/17 00:00 [pmc-release]
AID - S2214-7500(22)00039-7 [pii]
AID - 10.1016/j.toxrep.2022.03.012 [doi]
PST - epublish
SO  - Toxicol Rep. 2022 Mar 17;9:513-520. doi: 10.1016/j.toxrep.2022.03.012. 
      eCollection 2022.