PMID- 35356750
OWN - NLM
STAT- MEDLINE
DCOM- 20220401
LR  - 20220706
IS  - 1469-5073 (Electronic)
IS  - 0016-6723 (Print)
IS  - 0016-6723 (Linking)
VI  - 2022
DP  - 2022
TI  - Expression of LINC00847 in Peripheral Blood Mononuclear Cells of Children with 
      Asthma and Its Prediction between Asthma Exacerbation and Remission.
PG  - 5678257
LID - 10.1155/2022/5678257 [doi]
LID - 5678257
AB  - OBJECTIVE: Asthma is defined as a heterogeneous disease that is usually 
      characterized by chronic airway inflammation. Long noncoding RNAs play important 
      roles in various biological processes including inflammation. To know more about 
      the relationships between lncRNAs and asthma, we sought to the role of LINC00847 
      in peripheral blood mononuclear cells (PBMCs) of children with asthma 
      exacerbation or asthma remission. METHODS: Microarray analysis was performed on 
      GSE143192 and GSE165934 datasets to screen differentially expressed lncRNAs 
      (DElncRNAs) in human PBMCs between asthma patients and normal controls. LINC00847 
      was selected from DElncRNAs in human PBMCs between asthma patients and normal 
      controls for further investigation. The expression levels of LINC00847 were 
      quantified in PBMCs collected from 54 children with asthma exacerbation, 54 
      children with asthma remission, and 54 healthy children by real-time qPCR. The 
      forced expiratory volume in the first second in percent predicted values (FEV1%), 
      ratio of forced expiratory volume in 1 second to forced vital capacity 
      (FEV1/FVC), and peak expiratory flow rate (PEF%) were tested for evaluation of 
      lung function. The concentration of immunoglobulin E (IgE) and eosinophil count 
      was examined. The serum levels of interleukin-4 (IL-4), interferon-gamma (IFN-gamma), and 
      IL-17A were determined by the ELISA method. RESULTS: The expression level of 
      LINC00847 in PBMCs of asthma exacerbation children was remarkably higher than 
      that in PBMCs of asthma remission children and healthy children (p < 0.001); the 
      expression level of LINC00847 in PBMCs of asthma remission children was notably 
      higher than that in PBMCs of healthy children (p < 0.001). Pearson correlation 
      analysis revealed that the expression levels of LINC00847 in PBMCs of asthma 
      children were negatively correlated with FEV1% (r = -0.489), FEV1/FVC 
      (r = -0.436), PEF% (r = -0.626), and IFN-gamma level (r = -0.614) of asthma children, 
      but positively correlated with IgE concentration (r = 0.680), eosinophil count 
      (r = 0.780), IL-4 (r = 0.524), and IL-17A (r = 0.622) levels. When LINC00847 
      expression was used to distinguish asthma exacerbation from asthma remission, a 
      0.871 AUC (95% CI: 0.805-0.936) was yielded with sensitivity of 79.63% and 
      specificity of 77.78%. CONCLUSION: The study demonstrates that increased 
      LINC00847 expression may be associated with the development and progression of 
      asthma, possibly serving as a novel biomarker for predicting asthma exacerbation 
      from asthma remission.
CI  - Copyright (c) 2022 Jiaying Hu et al.
FAU - Hu, Jiaying
AU  - Hu J
AD  - Department of Pediatrics, Ningbo Yinzhou No. 2 Hospital, Ningbo City, Zhejiang 
      Province 315192, China.
FAU - Wang, Zhike
AU  - Wang Z
AD  - Department of Pediatrics, Ningbo Yinzhou No. 2 Hospital, Ningbo City, Zhejiang 
      Province 315192, China.
FAU - Han, Suzhen
AU  - Han S
AD  - Department of Pediatrics, Ningbo Yinzhou No. 2 Hospital, Ningbo City, Zhejiang 
      Province 315192, China.
FAU - Chen, Kai
AU  - Chen K
AUID- ORCID: 0000-0002-6305-5776
AD  - Department of Pediatrics, Ningbo Yinzhou No. 2 Hospital, Ningbo City, Zhejiang 
      Province 315192, China.
LA  - eng
PT  - Journal Article
DEP - 20220320
PL  - England
TA  - Genet Res (Camb)
JT  - Genetics research
JID - 101550220
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - *Asthma/genetics/metabolism
MH  - Child
MH  - Forced Expiratory Volume
MH  - Humans
MH  - *Leukocytes, Mononuclear/metabolism
MH  - *RNA, Long Noncoding/physiology
MH  - Respiratory Function Tests
MH  - Vital Capacity
PMC - PMC8958088
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/04/01 06:00
MHDA- 2022/04/02 06:00
PMCR- 2022/03/20
CRDT- 2022/03/31 05:36
PHST- 2022/01/26 00:00 [received]
PHST- 2022/02/26 00:00 [accepted]
PHST- 2022/03/31 05:36 [entrez]
PHST- 2022/04/01 06:00 [pubmed]
PHST- 2022/04/02 06:00 [medline]
PHST- 2022/03/20 00:00 [pmc-release]
AID - 10.1155/2022/5678257 [doi]
PST - epublish
SO  - Genet Res (Camb). 2022 Mar 20;2022:5678257. doi: 10.1155/2022/5678257. 
      eCollection 2022.